Abstract
In order to explore the effects of pomegranate peel polyphenols (PPPs) on SR-B1 protein expression and cholesterol efflux, in this study, Raw264.7 macrophages derived from foam cells were used as the model to illuminate the effects of PPPs on cholesterol efflux mediated by HDL. Western blot method was used to analyze the expression of scavenger receptor B1 (SR-B1), acyl-coenzyme A1: cholesterol acyltransferase (ACAT1) and cholesteryl ester hydrolase (nCEH). Further, the activity changes of ACAT1 and nCEH were revealed. The results showed that PPPs promoted the HDL-mediated cholesterol efflux and up-regulated the expression of SR-B1 significantly (P<0.05). In addition, the PPPs had no significant effects on nCEH and ACAT1 protein expression (P>0.05), but could significantly reduce the enzyme activity of ACAT1 and increase that of nCEH (P<0.05), which facilitated the transformation and outflow of intracellular cholesterol esters to free cholesterol.
Publication Date
2-28-2019
First Page
11
Last Page
14,91
DOI
10.13652/j.issn.1003-5788.2019.02.003
Recommended Citation
Shengjuan, ZHAO; Jinle, XIANG; Shuxing, CHEN; Peng, FEI; Yunfeng, XU; and Jianke, LI
(2019)
"Effects of pomegranate peel polyphenols on cholesterol efflux and related protein expression,"
Food and Machinery: Vol. 35:
Iss.
2, Article 3.
DOI: 10.13652/j.issn.1003-5788.2019.02.003
Available at:
https://www.ifoodmm.cn/journal/vol35/iss2/3
References
[1] YU Xiao-hua, FU Yu-chang, ZHANG Da-wei, et al. Foam cells in atherosclerosis[J]. Clinica Chimica Acta, 2013, 424: 245-252.
[2] MOORE K J, SHEEDY F J, FISHER E A. Macrophages in atherosclerosis: a dynamic balance[J]. Nature Reviews Immunology, 2013, 13: 709-721.
[3] GLASS C K, WITZTUM J L. Atherosclerosis: the road ahead[J]. Cell, 2001, 104(4): 503-516.
[4] 徐留建, 杨小东, 张剑梅, 等. 细胞胆固醇流出通路研究进展[J]. 中国心血管病研究, 2012, 10(4): 309-312.
[5] 梁俊. 石榴皮多酚对脂质过氧化及肝细胞HMG-CoA还原酶活性及表达的影响[D]. 西安: 陕西师范大学, 2012: 1-3.
[6] ROM O, AVIRAM M. Paraoxsonase2 (PON2) and oxidative stress involvement in pomegranate juice protection against cigarette smoke-induced macrophage cholesterol accumulation[J]. Chemico-Biological Interactions, 2016, 259(Pt B): 394-400.
[7] 马倩倩. 石榴皮多酚对肝细胞胆固醇合成影响及其分子机制研究[D]. 西安: 陕西师范大学, 2013: 21-26.
[8] QIN Li, YANG Yun-bo, YANG Yi-xin, et al. Inhibition of macrophage-derived foam cell formation by ezetimibe via the caveolin-1/MAPK pathway[J]. Clinical and Experimental Pharmacology and Physiology, 2016, 43(2): 182-192.
[9] JAMES R W, BRULHART-MEYNET M C, SINGH A K, et al. The scavenger receptor class B, type I is a primary determinant of paraoxonase-1 association with high-density lipoproteins[J]. Arteriosclerosis Thrombosis & Vascular Biology, 2010, 30(11): 2 121-2 127.
[10] 弓伊宁, 李芙蓉, 姚伟娟. 巨噬细胞的胆固醇代谢及其影响因素[J]. 生理科学进展, 2016, 47(4): 291-294.
[11] SEKIYA M, OSUGA J, IGARASHI M, et al, Ishibashi S The role of neutral cholesterol ester hydrolysis in macrophage foam cells[J]. Journal of Atherosclerosis and Thrombosis, 2011, 18(5): 359-364.
[12] DURHAM K K, CHATHELY K M, MAK K C, et al. HDL protects against doxorubicin-induced cardiotoxicity in a scavenger receptor class B type 1-, PI3K-, and Akt-dependent manner[J]. American Journal of Physiology-Heart and Circulatory Physiology, 2018, 314(1): H31-H44.
[13] ZHAO Sheng-juan, LI Jian-ke, WANG Li-fang, et al. Pomegranate peel polyphenols inhibit lipid accumulation and enhance cholesterol efflux in raw264.7 macrophages[J]. Food & Function, 2016, 7(7): 3 201-3 210.
[14] 周云, 沃兴德, 卢德赵. RAW264.7巨噬细胞源性泡沫细胞模型的建立及鉴定[J]. 中国动脉硬化杂志, 2010, 18(9): 687-690.
[15] 刘庆艳, 白静, 赵宇, 等. 白细胞介素34对巨噬细胞泡沫化过程中细胞内胆固醇水平的影响[J]. 中华老年心脑血管病杂志, 2017, 19(1): 16-19.
[16] 何雪峰, 李晓辉, 李淑慧, 等. 三七总皂苷对小鼠巨噬细胞源性泡沫细胞形成的影响[J]. 中国药房, 2007, 18(30): 2 323-2 325.