Simultaneous UPLC-MS/MS analysis of lovastatin and its analogue for functional foods

Authors

HAN Zhi, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
GONG Lei, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
WANG Huixia, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
ZHU Xiaoling, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
WANG Bin, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
CAO Qi, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
WU Wanqin, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China

Abstract

A ultra-high performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) assay method has been developed and fully validated for the simultaneous quantification of lovastatin, simvastatin, mevastatin, lovastatin sodium hydroxide, dehydro lovastatin. The samples were extracted with methanol and separated on a Waters Acquity HSS T3 column. The mobile phase consisting of 0.1% aqueous formic acid solution and acetonitrile using gradient elution at a flow rate of 0.3 mL/min. The positive and negative ions were scanned simultaneously. The multiple reaction monitoring (MRM) mode was used for detection. The results showed that the five compounds were separated well within five minutes. The limits of detection and the limits of quantification were 15 μg/kg and 50 μg/kg, respectively. The recoveries were between 94.6%~105.7%, and the relative standard deviation of the methods were lower than 10%. 17 batches of hypolipidemic health food were tested by this method, lovastatin, lovastatin sodium hydroxide salt and dehydro lovastatin were detected in one batch of samples. The method was simple, rapid, sensitive and accurate, and it was suitable for the rapid determination of lovastatin and its analogues in functional foods.

Publication Date

3-28-2019

First Page

58

Last Page

62

DOI

10.13652/j.issn.1003-5788.2019.03.010

Recommended Citation

Zhi, HAN; Lei, GONG; Huixia, WANG; Xiaoling, ZHU; Bin, WANG; Qi, CAO; and Wanqin, WU (2019) "Simultaneous UPLC-MS/MS analysis of lovastatin and its analogue for functional foods," Food and Machinery: Vol. 35: Iss. 3, Article 10.
DOI: 10.13652/j.issn.1003-5788.2019.03.010
Available at: https://www.ifoodmm.cn/journal/vol35/iss3/10

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