Detection of hydroxyapatite in milk powder

Authors

ZHU Lixian, Key Laboratory of Product Packaging and Logistics, Institute of Packaging Engineering, Jinan University, Zhuhai, Guangdong 519070, China
LIN Qinbao, Key Laboratory of Product Packaging and Logistics, Institute of Packaging Engineering, Jinan University, Zhuhai, Guangdong 519070, China
ZHONG Huaining, Guangdong Provincial Key Laboratory of Import and Export Technical Measures of Animal, Plant and Food, Guangdong Inspection and Quarantine Technology Center, Guangzhou， Guangdong 51632, China
WANG Liang, Guangdong Provincial Key Laboratory of Import and Export Technical Measures of Animal, Plant and Food, Guangdong Inspection and Quarantine Technology Center, Guangzhou， Guangdong 51632, China
PAN Jingjing, Guangdong Provincial Key Laboratory of Import and Export Technical Measures of Animal, Plant and Food, Guangdong Inspection and Quarantine Technology Center, Guangzhou， Guangdong 51632, China; Department of Food Science and Engineering, Jinan University,

Abstract

The milk powder was dissolved in ultrapure water, and the inorganics-rich components in the milk powder were extracted after ultrasonication, centrifugation and vacuum freeze-drying, and the inorganics-rich components in the milk powder were qualitatively analyzed by X-ray powder diffraction (XRD) and Fourier transform infrared spectroscopy. In addition, different concentrations of needle-like nano-hydroxyapatite reference standard were added to the blank milk powder sample, and the same prelimina-ry treatment and analysis as the milk powder samples was carried out. The morphology and nano-size of the inorganics-rich components in sample 1 and spiked sample were characterized by scanning electron microscopy (SEM) and transmission electron microscopy connected with energy spectrometer (TEM-EDS). The results showed that the spiked samples were determined to contain hydroxyapatite component in the XRD test. The needle-like nano-hydroxyapatite can be observed under electron microscope, thus verifying the feasibility of this method for detecting nano-hydroxyapatite. Among five samples, one sample contained hydroxyapatite in the XRD test and a spherical nano-component with unknown structure (the element composition was C, O, P, Cl); while three samples contained crystalline calcite and one sample did not contained crystalline hydroxyapatite.

Publication Date

3-28-2019

First Page

67

Last Page

71

DOI

10.13652/j.issn.1003-5788.2019.03.012

Recommended Citation

Lixian, ZHU; Qinbao, LIN; Huaining, ZHONG; Liang, WANG; and Jingjing, PAN (2019) "Detection of hydroxyapatite in milk powder," Food and Machinery: Vol. 35: Iss. 3, Article 12.
DOI: 10.13652/j.issn.1003-5788.2019.03.012
Available at: https://www.ifoodmm.cn/journal/vol35/iss3/12

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