Abstract
The bacteriostatic action and mechanism of both against Bacillus cereus were studied by using ε-polylysine hydrochloride and nisin alone and in combination. The alkaline phosphatase content, UV absorbing content, pyruvic acid content and SDS-PAGE gel electrophoresis were used as indicators to comprehensively evaluate the antibacterial activity of ε-polylysine hydrochloride and Nisin against Bacillus cereus. The results showed that ε-polylysine hydrochloride and Nisin had a synergistic inhibitory effect on Bacillus cereus. Nisin mainly destroyed cell wall membrane structure and increased the permeability of cell wall membrane, while ε-polylysine hydrochloride mainly affected the growth and metabolism of bacteria by affecting ATPase activity, protein synthesis and expression, and energy metabolism. The combination use of the ε-polylysine hydrochloride and nisin can achieve better bacteriostatic effect at lower concentrations.
Publication Date
3-28-2019
First Page
15
Last Page
19,103
DOI
10.13652/j.issn.1003-5788.2019.03.003
Recommended Citation
Wenyan, SHI and Zhen, SUN
(2019)
"Synergistic effect and mechanism of ε-polylysine hydrochloride and Nisin on Bacillus cereus,"
Food and Machinery: Vol. 35:
Iss.
3, Article 3.
DOI: 10.13652/j.issn.1003-5788.2019.03.003
Available at:
https://www.ifoodmm.cn/journal/vol35/iss3/3
References
[1] DUNCAN C L, FOSTER E M. Effect of sodium nitrite sodium chloride, and sodium nitrate on germination and outgrowth of anaerobic spores[J]. Applied Microbiology, 1968, 16(2): 406-411.
[2] DUNCAN C L, FOSTER E M. Role of curing agents in the preservation of shelf-stable canned meat product[J]. Applied Microbiology, 1968, 16(2): 401-405.
[3] JR J B F. Chemistry of Meat Pigments[J]. Agricultural and Food Chem, 1966, 14(3): 207-210.
[4] FREYBLER L A, GRAY J I, ASGHAR A, et al. Nitrite stabilization of lipids in cured pork[J]. Meat Science, 1993, 33(1): 85-96.
[5] RICHRDS M P. Redox Reactions of Myoglobin[J]. Antioxidants & Redox Signaling, 2013, 18(17): 2 342-2 351.
[6] BRUING-FANN C S, KANEENE J B. The effects of nitrate, nitrite and N-nitroso compounds on human health: a review[J]. Veterinary & Human Toxicology, 1993, 35(6): 521， 38.
[7] CHENNI F Z, TACH, NAUD N, et al. Heme-induced Biomarkers associated with red meat promotion of colon Cancer Are Not Modulated by the intake of nitrite[J]. Nutrition and Cancer, 2013, 65(2): 227-233.
[8] BOUVARD V, LOOMIS D, GUYTON K Z, et al. Carcinogenicity of consumption of red and processed meat[J]. The Lancet Oncology, 2015, 16(16): 1 599-1 600.
[9] HYLDGAARD M, MYGIND T, VAD B S, et al. The Antimicrobial Mechanism of Action of Epsilon-Poly-L-Lysine[J]. Applied and Environmental Microbiology, 2014, 80(24): 7 758-7 770.
[10] 刘韵昕. 苯乳酸的抑菌活性及抑菌机理研究[D]. 临汾: 山西师范大学, 2017: 21.
[11] LIN lin, GU Yu-lei, LI Chang-zhu, et al. Antibacterial mechanism of ε-Poly-lysine against Listeria monocytogenes and its application oncheese[J]. Food Control, 2018, 91(9): 76-84.
[12] 王佶. 大肠杆菌积累丙酮酸的研究[D]. 杭州: 浙江大学，2008: 23-25.
[13] 翟培, 韩晋辉, 侯丽霞. 家蝇抗菌肽的抑菌动力学研究及其机理初探[J]. 中国生物工程, 2006, 26(11): 33-39.
[14] 刘蔚, 周涛. ε-聚赖氨酸抑菌机理研究[J]. 食品科学, 2009, 30(9): 15-20.
[15] CHEN C Z, COOPER S L. Interactions between dendrimer biocides and bacterial membranes[J]. Biomaterials, 2002, 23(16): 3 359-3 368.
[16] PAPARELLA A, TACCOGNA L, AGUZZI I, et al. Flow cytometric assessment of the antimicrobial activity of essential oils against Listeria monocytogenes[J]. Food Control, 2008, 19(12): 1 174-1 182.
[17] WICZLING P, KRZYZANSKI W. Flow cytometric assessment of homeostatic aging of reticulocytes in rats[J]. Experimental Hematology, 2008, 36(2): 119-127.