Optimization of two luteolin derivatives preparation from artichoke by HPLC

Authors

SHI Mingyue, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
CAO Qingming, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
LI Qun, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
ZHONG Wenhui, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
WANG Yuanqing, Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
LIU Zhiwen, Huimei Agricultural Science and Technology Co., Ltd, Changde, Hunan 415137, China
ZHANG Xiping, Changde Agriculture and Forestry Research Institute, Changde, Hunan 415000, China

Abstract

This study describes a method for isolating two luteolin glycosides from artichokes（Cynara scolymus L.）by preparative liquid chromatography. The partion A27~29 (4 g) of 50% ethanol eluent was achieved by two steps, 70% ethanol extract of artichoke adsorbed by AB-8 macroporous resin and eluted with 20% ethanol, 50% ethanol and 80% ethanol successively. 0.32 g of target part C16~19 with target components H-01 (10.4%) and H-02 (5.1%) was achieved by two steps, A27~29 adsorbed by RP-C18 stationary phase column (270 mm×80 mm, 40~60 μm) and eluted with 30%, 48% and 100% methanol successively. Taking the resolution and shape of target peaks as optimization conditions, the effects of five different mobile phase combinations and two kinds of flow rates on the separation of target peaks were studied. Based on the optimal conditions, H-01 and H-02 with purity of 97.8% and 96.5%, respectively, were obtained. H-01 and H-02 were identified as luteolin-7-rutinoside and luteolin-7-O-β-D-glucoside, respectively, by mass spectrometry, ¹H-NMR and ¹³C-NMR analysis. This method can effectively reduce the impurity level under the condition of high recovery, and provide a new method for the development of the separation and purification process of luteolin compounds.

Publication Date

3-28-2019

First Page

176

Last Page

181

DOI

10.13652/j.issn.1003-5788.2019.03.032

Recommended Citation

Mingyue, SHI; Qingming, CAO; Qun, LI; Wenhui, ZHONG; Yuanqing, WANG; Zhiwen, LIU; and Xiping, ZHANG (2019) "Optimization of two luteolin derivatives preparation from artichoke by HPLC," Food and Machinery: Vol. 35: Iss. 3, Article 32.
DOI: 10.13652/j.issn.1003-5788.2019.03.032
Available at: https://www.ifoodmm.cn/journal/vol35/iss3/32

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