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Abstract

The 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH·), hydroxyl free radical (OH·), 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) free radical (ABTS+·) and superoxide radical (O-2·) scavenging ability were selected as the four indicators to evaluate the in vitro antioxidant properties of different selenium-containing substances, and compared with selenium-enriched yeast (SY) and non-selenium-enriched peptides and inorganic selenium mixture (SPIS).The results showed that SPI had the strongest ability to scavenge four kinds of free radicals, and its DPPH·, OH·, ABTS+· and O-2· scavenging rates were 87.5%, 50.3%, 99.7% and 45.7%. The weakest sample was IS, and its radicals scavenging rates was no more than 14%. By comparing the scavenging rates and semi-inhibitory concentration (IC50) of four radicals of SPR, SPI, CE, SY, IS and SPIS, it was found that the ability to scavenge free radicals of organic selenium was stronger than inorganic selenium.

Publication Date

4-28-2019

First Page

174

Last Page

178

DOI

10.13652/j.issn.1003-5788.2019.04.032

References

[1] NATHALIE P, ALAIN L, MATHIEU R, et al. Selenoprotein N: an endoplasmic reticulum glycoprotein with an early developmental expression pattern[J]. Human Molecular Genetics, 2003, 12(9): 1 045-1 053.
[2] WHANGER P. Selenoprotein W: a review[J]. Cellular and Molecular Life Sciences, 2001, 57(13): 1 846-1 852.
[3] COMBS G, GRAY W. Chemopreventive Agents: Selenium[J]. Pharmacology and Therapeutics, 1998, 79(3): 179-192.
[4] CHU F F, DOROSHOW J H, ESWORTHY R S. Expression, characterization, and tissue distribution of a new cellular selenium-dependent glutathione peroxidase, GSHPx-GI[J]. Journal of Biological Chemistry, 1993, 268(4): 2 571-2 576.
[5] 潘洁敏, 贾伟平. 硒蛋白P的研究进展[J]. 中华医学杂志, 2013, 93(10): 794-796.
[6] 雷红灵, 方响亮, 周大寨. 堇叶碎米荠硒蛋白的体内抗氧化作用研究[J]. 西北农林科技大学学报: 自然科学版, 2015, 43(1): 201-205.
[7] 丁文靖, 刘力, 祝晓蕾. 硒酵母的研制及分析[J]. 食品与发酵工业, 1992(5): 73-74, 72.
[8] 匡恩俊, 迟凤琴, 张久明, 等. 叶面喷硒对不同作物籽粒硒含量及产量的影响[J]. 中国土壤与肥料, 2018(4): 139-142.
[9] 付永霞, 黄歆妤, 胡锦蓉, 等. 加工方式对富硒大豆制品中硒含量的影响[J]. 食品科技, 2018, 43(8): 170-175.
[10] 石爱华, 黄思勇, 陈体强, 等. 人工栽培堇叶碎米荠生物学特征及其营养成分比较[J]. 中国野生植物资源, 2018, 37(1): 31-36.
[11] CUI Li-wei, ZHAO Jia-ting, CHEN Jiu-yu, et al. Translocation and transformation of selenium in hyperaccumulator plant Cardamine enshiensis from Enshi, Hubei, China[J]. Plant and Soil, 2018, 425(1): 577-588.
[12] 曹斌. 恩施碎米荠含硒蛋白分离提取、初步纯化、体外抗氧化活性研究[D]. 贵阳: 贵州大学, 2016: 35-51.
[13] YUAN Lin-xi, ZHU Yuan-yuan, LIN Zhi-qing, et al. A novel selenocystine-accumulating plant in selenium-mine drainage area in enshi, china[J]. Plos One, 2013, 8(6): e65615.
[14] 吕昊. 含硒蛋白运动补剂缓解运动性疲劳作用研究[J]. 食品研究与开发, 2016, 37(21): 172-175.
[15] 刘坤媛, 田秀丽, 秦治国. 壶瓶碎米荠中含硒蛋白结构特性及其缓解运动性疲劳的作用[J]. 食品科学, 2015, 36(9): 160-165.
[16] 包怡红, 王芳, 王文琼. 大豆多肽硒螯合物的制备及抗氧化活性分析[J]. 食品科学, 2013, 34(16): 27-32.
[17] 圣志存, 吴双, 王安平, 等. 珊瑚菌子实体和菌丝体营养成分与抗氧化活性的比较[J]. 现代食品科技, 2018, 34(5): 62-67, 40.
[18] 董竹平, 李超, 扶雄. 不同品种辣木叶多糖的理化性质和抗氧化活性研究[J]. 现代食品科技, 2018, 34(1): 38-44.
[19] 孙雪萍, 徐艳, 刘布鸣, 等. 沙虫蛋白酶解产物抗氧化与免疫调节活性的研究[J]. 现代食品科技, 2017, 33(4): 67-72.
[20] 孙亚男, 李文香, 胡欣蕾, 等. 杏鲍菇多肽提取工艺优化及其抗氧化活性研究[J]. 食品与机械, 2017, 33(4): 144-149.
[21] 王梅, 张红香, 邹志辉, 等. 原子荧光光谱法测定富硒螺旋藻片中不同形态、价态的硒[J]. 食品科学, 2011, 32(6): 179-182.
[22] 姚昭, 李红艳, 张云龙, 等. 有机硒、无机硒、VE单独使用及有机硒与VE联用对大鼠体内抗氧化能力的影响[J]. 食品科学, 2013, 34(15): 272-276.
[23] 郑时莲, 潘瑶, 张云龙, 等. 不同形态硒、维生素E、紫萝卜提取物体外抗氧化协同作用研究[J]. 食品工业科技, 2016, 37(19): 86-90.
[24] 李改平, 刘子川, 王梦亮. 有机硒和无机硒对小鼠抗氧作用比较研究[J]. 山西中医学院学报, 2001, 2(3): 19-21.
[25] ZHANG Zhong-hao, WU Qiu-yan, CHEN Chen, et al. Comparison of the effects of selenomethionine and selenium-enriched yeast in the triple-transgenic mouse model of Alzheimer's disease[J]. Food and Function, 2018, 9(7): 3 965-3 973.
[26] 黄延盛, 吴小勇, 宁初光, 等. 富硒大豆蛋白的酶解及抗氧化性能研究[J]. 中国食品工业, 2013(8): 63-66.

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