Abstract
A method for identification of glucosinolate in three kinds of cruciferous vegetable leaves was developed by using HPLC-Q-TOF-MS. Firstly, the mass spectrometry fragmentation pathways of two standard glucosinolates was studied. The bond of glucose, sulfated thiohydroximate and R-group was easily fractured and the rearrangement reaction was observed for the forming the fragment ions. Then, the structure of 8 glucosinolates including 3-(methylsulfinyl) propyl (1), 4-(methylsulfinyl) butyl (2), 3-butenyl (3), 4-hydroxybenzyl (4), 1-hydroxyindol-3-ylmethyl (5), Indol-3-ylmethyl (6), 1-methoxyindol-3-ylmethyl (7), 6-(methylsulfonyl) hexyl glucosinolate (8) were identified based on the fragmentation pathways of two standard glucosinolates, There were 4 kinds of glucosinolate (1, 2, 4, 5) in Baota Broccoli, 6 kinds of glucosinolate (2, 3, 5, 6, 7, 8) in Qinghuacai and 7 kinds of glucosinolate(1, 2, 4, 5, 6, 7) in Zise Broccoli.
Publication Date
4-28-2019
First Page
42
Last Page
47,119
DOI
10.13652/j.issn.1003-5788.2019.04.008
Recommended Citation
Pei, PENG; Tianyan; Fangming, DENG; and Zhixing, QING
(2019)
"Identification of glucosinolate in different varieties of broccoli leaves baesd on HPLC-Q-TOF-MS technology,"
Food and Machinery: Vol. 35:
Iss.
4, Article 8.
DOI: 10.13652/j.issn.1003-5788.2019.04.008
Available at:
https://www.ifoodmm.cn/journal/vol35/iss4/8
References
[1] KATSAROU D, OMIROU M, LIADAKI K, et al. Glucosinolate biosynthesis in Eruca sativa[J]. Plant Physiology & Biochemistry, 2016, 109: 452-466.
[2] 陈新娟, 朱祝军, 钱琼秋, 等. 硫代葡萄糖苷的提取、分离和鉴定[J]. 中国食品学报, 2007, 7(3): 43-48.
[3] GROSSER K, VAN DAM N M. A Straightforward method for glucosinolate extraction and analysis with high-pressure liquid chromatography (HPLC)[J]. Journal of Visualized Experiments, 2017, 121: 2-9.
[4] CLARKE D B. Glucosinolates structures and analysis in food[J]. Analytical Methods, 2010, 2(4): 310-325.
[5] 马越, 丁云花, 刘光敏, 等. 青花菜花球及叶片中硫代葡萄糖苷组分及含量分析[J]. 江苏农业科学, 2016, 44(7): 300-303.
[6] 赵振东, 李嘉诚, 冯玉红, 等. 超高效液相色谱—串联质谱法鉴别十字花科植物中硫代葡萄糖苷[J]. 化学分析计量, 2013, 22(2): 12-15.
[7] 杨艳婧. 西兰苔籽中异硫氰酸盐的提取与抗肿瘤活性研究[D]. 广州: 华南理工大学, 2010: 1-81.
[8] BENNETT R N, MELLON F A, BOTTING N P, et al. Identification of the major glucosinolate (4-mercaptobutyl glucosinolate) in leaves of Eruca sativa L. (salad rocket)[J]. Phytochemistry, 2002, 61(1): 25-30.
[9] FABRE N, POINSOT V, DEBRAUWER L, et al. Characterisation of glucosinolates using electrospray ion trap and electrospray quadrupole time-of-flight spectrometry[J]. Phytochemistry Analysis, 2010, 18(4): 306-319.
[10] KIM H W, KO H C, BAEK H J, et al. Identification and quantification of glucosinolates in Korean leaf mustard germplasm (Brassica juncea var. integrifolia) by liquid chromatography-electrospray ionization/tandem mass spectrometry[J]. European Food Research & Technology, 2016, 242(9): 1 479-1 484.
[11] 郑姝宁, 张延国, 郭宁, 等. 羽衣甘蓝和白菜型油菜硫代葡萄糖苷超高效液相色谱—飞行时间质谱分析方法[J]. 园艺学报, 2016, 43(12): 2 481-2 490.