Simultaneous determination of nine female-steroid hormones in brown sugar by UPLC-MS/MS

Authors

HAN Zhi, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
WANG Huixia, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
GONG Lei, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
ZHU Xiaoling, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
CAO Qi, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
WANG Bin, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
ZHANG Miao, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China

Abstract

A ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for the determination of female-steroid hormones in brown sugar. Brown sugar samples were dissolved in water, and extracted by acetonitrile, then cleaned-up using an HLB column. The analytes were separated on a ACQUITY UPLC HSS T3 column with acetonitrile-water (containing 2 mmol/L ammonium acetate) as mobile phase by gradient elution. The mass spectrometer was detected in multiple reaction monitoring mode(MRM) using positive-ion and negative-ion electrospray ionizations(ESI), respectively. The quantitative analysis was carried out by external standard method. The results showed that the calibration curves had good linearity for 9 female-steroid hormones in the tested concentration ranges, with correlation coefficients of more than 0.99. The average recoveries at three spiked levels were 92.2%~113.2%, and the precisions expressed as relative standard deviations (RSDs) were 1.6%~6.8%. The limits of detection (LOD) were 0.2~1.0 μg/kg. The method proved to be accurate, rapid, sensitive with repeatability and suitable for rapid detection of female hormone in brown sugar.

Publication Date

5-28-2019

First Page

96

Last Page

100

DOI

10.13652/j.issn.1003-5788.2019.05.017

Recommended Citation

Zhi, HAN; Huixia, WANG; Lei, GONG; Xiaoling, ZHU; Qi, CAO; Bin, WANG; and Miao, ZHANG (2019) "Simultaneous determination of nine female-steroid hormones in brown sugar by UPLC-MS/MS," Food and Machinery: Vol. 35: Iss. 5, Article 17.
DOI: 10.13652/j.issn.1003-5788.2019.05.017
Available at: https://www.ifoodmm.cn/journal/vol35/iss5/17

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