Rapid screening and quantitative analysis of 73 kinds of chemicals illegally added in the healthy foods by Orbitrap mass spectrometry

Authors

HAN Zhi, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
GONG Lei, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
WANG Huixia, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
ZHU Xiaoling, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
WU Wanqin, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
LIU Guojiao, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
WANG Bin, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China

Abstract

A high-throughput qualitative screening and quantita-tive method of ultra-high performance liquid chromatography combined with orbitrap mass spectrometry（UPLC-Orbitrap-MS） was developed to determine 73 kinds of chemicals illegally added in the healthy foods. The samples were extracted with methanol using ultrasonic extraction. The separation was eluted by gradient on a Waters Acquity UPLC HSS T3 column (100 mm×2.1 mm, 1.8 μm), with a mobile phase consisting of water (0.1% formic acid) and acetonitrile in both positive and negative mode. The data were acquired via full-scan data-dependent MS/MS (full scan-ddMS²). The qualitative analyses of the compounds were performed through the database established by Trace Finder software, and the quantitative detection were undertaken through the area of precursor ions. The results showed that each target had good linearity in its own concentration range, and the correlation coefficients were higher than 0.99. The limits of determination (LODs) were between 2～20 μg/kg, and the limits of quantification (LOQs) were between 6～60 μg/kg. The recoveries of the proposed method were in range of 72.2%～117.5% at spiked levels of 100, 200, and 1 000 μg/kg with deviations (RSDs) between 2.3%～6.8%. It could be concluded that the high-throughput, qualitative and quantitative method reported in this study was useful for the determination of illicit drugs in functional foods.

Publication Date

7-28-2019

First Page

74

Last Page

79,116

DOI

10.13652/j.issn.1003-5788.2019.07.013

Recommended Citation

Zhi, HAN; Lei, GONG; Huixia, WANG; Xiaoling, ZHU; Wanqin, WU; Guojiao, LIU; and Bin, WANG (2019) "Rapid screening and quantitative analysis of 73 kinds of chemicals illegally added in the healthy foods by Orbitrap mass spectrometry," Food and Machinery: Vol. 35: Iss. 7, Article 13.
DOI: 10.13652/j.issn.1003-5788.2019.07.013
Available at: https://www.ifoodmm.cn/journal/vol35/iss7/13

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