Abstract
An analytical method was developed for the determination of 8 kinds of vitamin E isomer (tocopherol and tocotrienol) in vegetable oil by solvent dilution extraction-ultra performance liquid chromatography. The sample was extracted with acetone. The separation was achieved by using PFP column and an elution with the mobile phases of methanol and water (93∶7) at a flow rate of 0.4 mL/min. The identification and quantitative analysis were carried out with fluorescence detector at an ecxitation wavelength of 295 nm and an emission wavelength of 330 nm. The recoveries ranged from 90.0% to 100%, with the relative standard deviation (RSDs) between 0.28% and 1.22%. The linear range was 0.4～40.0 μg/mL and the correlation coefficient (r) was greater than 0.999. The limits of detection were 4.0 mg/kg for each vitamin E isomer. The method was enough for the analysis of vitamin E in actual samples with wide linear range and high sensitivity.
Publication Date
8-28-2019
First Page
73
Last Page
76,99
DOI
10.13652/j.issn.1003-5788.2019.08.014
Recommended Citation
Xin, DONG
(2019)
"Simultaneous determination of 8 kinds of vitamin E isomer in vegetable oil using reversed-phase ultra performance liquid chromatography,"
Food and Machinery: Vol. 35:
Iss.
8, Article 14.
DOI: 10.13652/j.issn.1003-5788.2019.08.014
Available at:
https://www.ifoodmm.cn/journal/vol35/iss8/14
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