HPLC analysis of flavonoids in Apocynumvenetum tea and its antioxidation in vitro

Authors

ZHANG Jing, Environment and Quality Inspection College, Chongqing Chemical Industry Vocational College, Chongqing 401228, China
REN Xiaoli, Environment and Quality Inspection College, Chongqing Chemical Industry Vocational College, Chongqing 401228, China
LI Xixi, Environment and Quality Inspection College, Chongqing Chemical Industry Vocational College, Chongqing 401228, China
YI Sha, Environment and Quality Inspection College, Chongqing Chemical Industry Vocational College, Chongqing 401228, China
LI Chong, Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China
ZHAO Xin, Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China

Abstract

In this study, the contents of rutin and isoquercetin in flavonoids of Apocynumvenetum tea was determined by high performance liquid chromatography (HPLC), and the antioxidant effect in vitro was evaluated. A good separation of rutin and isoquercetin from flavonoids of Apocynumvenetum tea was achieved by vacuum heating reflux combined with macroporous adsorption. Rutin and isoquercetin were separated effectively in the extract of Apocynum venetum teaflavonoids. The results showed good linear relationship, the range of rutin was 0.453 1~9.062 5 μg (r=0.999 55) and that of isoquercetin ranged from 0.258 3 to 5.166 5 μg (r=0.999 65). The recoveries of rutin and isoquercetin were 99.91% and 100.05% (n=9). The accuracy RSD of this method were respectively 0.11% and 0.10% (n=5). The contents of rutin and isoquercetin in Apocynum venetum tea flavonoids were 0.059 4, 0.673 4 mg/g respectively. Moreover, the antioxidant experimental results showed that Apocynum venetum tea flavonoids had higher scavenging abilities of 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl radicals than V_C positive control. It can be found that rutin and isoquercitrinwere the main components of flavonoids in Apocynumvenetum tea. The flavonoids in Apocynumvenetum tea had good antioxidant activitiesin vitro.

Publication Date

9-28-2019

First Page

89

Last Page

93

DOI

10.13652/j.issn.1003-5788.2019.09.017

Recommended Citation

Jing, ZHANG; Xiaoli, REN; Xixi, LI; Sha, YI; Chong, LI; and Xin, ZHAO (2019) "HPLC analysis of flavonoids in Apocynumvenetum tea and its antioxidation in vitro," Food and Machinery: Vol. 35: Iss. 9, Article 17.
DOI: 10.13652/j.issn.1003-5788.2019.09.017
Available at: https://www.ifoodmm.cn/journal/vol35/iss9/17

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