Abstract
In this paper, the lung cell A549 was used as a model. The protective effect of camphor (C. camphora) leaf essential oil on the A549 exposed to PM2.5 was investigated. Less than 1 mg/mL of essential oil had no effect on A549 cell proliferation, and low concentration had a certain promotion effect. 0.05～1.00 mg/mL essential oil had a significant antagonistic effect on cytotoxicity induced by PM2.5 (P＜0.05). The essential oilsignificantly reduced the content of MDA in the infected cells and increased the activity of SOD and CAT and the content of T-AOC (P＜0.05), indicating that the camphor leaf essential oil could antagonize the cytotoxicity of PM2.5 through antioxidant stress. The camphora leaf essential oilsignificantly reduced the content of TNF-α and IL-6 in the infected cells (P＜0.05), indicating that the camphor leaf essential oil could antagonize the cytotoxicity of PM2.5 by inhibiting the inflammatory reaction.
Publication Date
9-28-2019
First Page
169
Last Page
172
DOI
10.13652/j.issn.1003-5788.2019.09.033
Recommended Citation
Guohong, XIONG; Hai, LIN; Jianguo, HE; Yinghong, LI; and Xiangjin, FU
(2019)
"Antagonistic effect of camphor leaf essential oil on damage induced by PM2.5 in lung epithelial cells A549,"
Food and Machinery: Vol. 35:
Iss.
9, Article 33.
DOI: 10.13652/j.issn.1003-5788.2019.09.033
Available at:
https://www.ifoodmm.cn/journal/vol35/iss9/33
References
[1] 翟文慧, 黄志刚, 冯聪, 等. 大气细颗粒污染物PM2.5浓度及对肺上皮细胞炎性因子的影响[J]. 现代生物医学进展, 2015, 15(6): 1 028-1 031.
[2] YANG La-wei, WANG Ya-hong, LIN Zi-ying, et al. Mitochondrial OGG1 protects against PM2.5-induced oxidative DNA damage in BEAS-2B cells[J]. Experimental and Molecular Pathology, 2015,99(2): 365-373.
[3] CHRISTIANSEN J G, JENSEN H E, JENSEN L K, et al. Systemic inflammatory response and local cytokine expression in porcine models of endocarditis[J]. APMIS, 2014, 122(4): 292-300.
[4] 刘涛, 阮研硕, 毕婷婷, 等. 植物活性物质对PM2.5致细胞损伤的抗性作用[J]. 环境科学研究, 2015(11): 1 677-1 686.
[5] BUCZYNSKA A J, KRATA A, VAN GRIEKEN R, et al. Composition of PM2.5 and PM1 on high and low pollution event days and its relation to indoor air quality in a home for the elderly[J]. Sci Total Environ,2014, 490C: 134-143.
[6] 姜薇, 赵晓红, 米生权, 等. 番茄红素对大气可吸入颗粒物致人肺成纤维细胞DNA 损伤的保护作用[J]. 环境与职业医学, 2008(6): 568-571.
[7] 晓开提·依不拉音, 克丽别娜·吐尔逊, 范妙莉, 等. 某地大气细颗粒物对大鼠肺损害与维生素E的拮抗作用[J]. 毒理学杂志, 2013, 27(4): 287-290.
[8] 王鹤潼. PM2.5引起急性肺炎的机制探讨及复方精油对其抗炎效果研究[D]. 大连: 大连医科大学, 2017: 16-25.
[9] 王臻, 王辰, 庞宝森. 大气可吸入颗粒物致大鼠肺损伤及N-乙酰半胱氨酸的保护作用研究[J]. 中华医学杂志, 2004, 84(6): 66-69.
[10] 祖桂芳, 姜薇, 赵晓红, 等. 天然提取物抗PM2.5诱导A549细胞凋亡的作用[J]. 生态毒理学报, 2011, 6(4): 429-434.
[12] 秦海燕, 叶平, 索志荣. 香樟精油自然挥发对肺炎小白鼠的保护作用[J]. 黑龙江畜牧兽医, 2015(11): 212-214.
[11] 丛赢, 张琳, 祖元刚, 等. 油樟(Cinnamomum longepaniculatum)精油的抗炎及抗氧化活性初步研究[J]. 植物研究, 2016, 36(6): 949-954, 960.
[13] 朱震, 王小娟, 邓晗依, 等. 丝素肽对过氧化氢致A549细胞损伤的修复作用[J]. 环境与职业医学, 2016, 33(3): 233-236.
[14] MURAPA P, DAI J, CHUNG M, et al. Anthocyanin-rich fractions of blackberry extracts reduce UV induced free radicals and oxidative damage in keratinocytes[J]. Phytother Res, 2012, 26(1): 106-112.
[15] 刘婷, 魏海英, 杨文妍, 等. 太原市冬季灰霾天气大气PM2.5对肺泡巨噬细胞的氧化损伤作用[J]. 环境科学学报, 2015, 35(3): 890-896.
[16] 翟文慧, 黄志刚, 冯聪, 等. 大气细颗粒污染物PM2.5浓度及对肺上皮细胞炎性因子的影响[J]. 现代生物医学进展, 2015, 15(6): 1 028-1 031.
[17] STIDHAM R W, LEE T C, HIGGINS P D, et al. Systematic review with network meta-analysis: The efficacy of anti-TNF agents for the treatment of Crohn's disease[J]. Aliment Pharmacol Ther, 2014, 39(12): 1 349-1 362.
[18] POZZI R, DE BERARDIS B, PAOLETTI L, et al. Inflammatory mediators induced by coarse (PM2.5-10) and fine (PM2.5) urban air particles in RAW 264.7 cells[J]. Toxicology, 2003, 183(1/2/3): 243-254.
[19] 姜瑞雪, 万运方, 刘雁云, 等. PM2.5对Wistar大鼠肺组织中HMGB1、TNF-α、IL-6表达的影响[J]. 环境卫生学杂志, 2014(6): 514-517.
[20] 焦周光, 付绪磊, 温占波, 等. 北京大气PM2.5对A549细胞炎性因子及DNA损伤的毒性[J]. 中国环境科学, 2016,36(5): 1 579-1 588.