Effect of β-sitosterol acetate ester on the structural stability of lipid membrane

Authors

HOU Lifen, College of Chemistry, Chemical and Environmental Engineering, Henan University of Technology, Zhengzhou, Henan 450001, China; Department of Cooking and Food, Zhengzhou Tourism College, Zhengzhou, Henan 450009, China
ZONG Shanying, China Certification & Inspection Group Central Plains Agricultural Products & Food Testing 〔Henan〕 Co., Ltd, Zhengzhou, Henan 450003, China
ZHANG Haichen, College of Grain, Oil and Food, Jilin Vocational College of Engineering, Siping, Jilin 136001, China
PAN Li, College of Food Science and Technology, Henan University of Technology, Zhengzhou, Henan 450001, China
WANG Hongyan, College of Chemistry, Chemical and Environmental Engineering, Henan University of Technology, Zhengzhou, Henan 450001, China
GU Keren, College of Chemistry, Chemical and Environmental Engineering, Henan University of Technology, Zhengzhou, Henan 450001, China; College of Grain, Oil and Food, Jilin Vocational College of Engineering, Siping, Jilin 136001, China

Abstract

β-sitosterol acetate liposomes were prepared by thin-film ultrasonic method, and the effects of different incorporation concentrations on the particle size, PDI and storage stability of the liposomes were investigated, besides, the interaction between β-sitosterol acetate ester and phospholipids in the membrane was explored. The results showed that the size and PDI of the liposomes were consistent with that of the β-sitosterol liposomes under the same conditions when the addition amount of β-sitosterol acetate ester was 2 mg and 4 mg. It has good storage stability for low dosage of the β-sitosterol acetate ester liposomes at 4 ℃ for 30 days. The results showed that there was interactions between β-sitosterol acetate ester and P═O and (CH₃)₃N of phospholipids by FTIR. There was a stabilizing effect on liposome membrane structure at low dosage of β-sitosterol acetate ester by TGA.

Publication Date

9-28-2019

First Page

20

Last Page

25

DOI

10.13652/j.issn.1003-5788.2019.09.005

Recommended Citation

Lifen, HOU; Shanying, ZONG; Haichen, ZHANG; Li, PAN; Hongyan, WANG; and Keren, GU (2019) "Effect of β-sitosterol acetate ester on the structural stability of lipid membrane," Food and Machinery: Vol. 35: Iss. 9, Article 5.
DOI: 10.13652/j.issn.1003-5788.2019.09.005
Available at: https://www.ifoodmm.cn/journal/vol35/iss9/5

References

[1] PATRICIA DA S M, DAROIT D J, BRANDELLI A. Food applications of liposome-encapsulated antimicrobial peptides[J]. Trends in Food Science & Technology, 2010, 21(6): 284-292.
[2] WU Jie-yu, GUAN Rong-fa, HUANG Hai-zhi, et al. Effect of catechin liposomes on the nitrosamines and quality of traditional Chinese bacon[J]. Food & Function, 2019, 10(2): 625-634.
[3] TIAN Meng-meng, HAN Jian-zhong, YE Ai-qian, et al. Structural characterization and biological fate of lactoferrin-loaded liposomes during simulated infant digestion[J]. Journal of the Science of Food and Agriculture, 2019, 99(4): 2 677-2 684.
[4] KIRBY C J, WHITTLE C J, RIGBY N, et al. Stabilization of ascorbic acid by microencapsulation in liposomes[J]. International Journal of Food Science & Technology, 2010, 26(5): 437-449.
[5] DANG Qi-feng, LIU Cheng-sheng, YAN Jing-qua, et al. The preparation and characterization of CMCS-coated VE liposo-me[J]. Journal of Functional Materials, 2011, 235(3): 741-745.
[6] MARSANASCO M, CALABR V, PIOTRKOWSKI B, et al. Fortification of chocolate milk with omega-3, omega-6 and vitamins E and C by using liposomes[J]. European Journal of Lipid Science & Technology, 2016, 118(9): 1 271-1 281.
[7] BARENHOLZ Y. Liposome application: Problems and prospects[J]. Current Opinion in Colloid & Interface Science, 2001, 6(1): 66-77.
[8] CHIAHUNG W, BLALECHAFANALl M, NEWMAN D K. Methylation at the C-2 position of hopanoids increases rigidity in native bacterial membranes[J]. Elife, 2015, 4(4): 1-18.
[9] NAJAFINOBAR N, MELLANDER L J, KURCAY M E, et al. Cholesterol alters the dynamics of release in protein independent cell models for exocytosis[J]. Scientific reports, 2016, 6: 33 702.
[10] MIAO Zhi-lin, DENG Ying-jie, DU Hong-yan, et al. Preparation of a liposomal delivery system and its in vitro release of rapamycin[J]. Experimental and Therapeutic Medicine, 2015, 9(3): 941-946.
[11] TAKECHI-HARAYA Y, SAKAI-KATO K, ABE Y. Atomic force microscopic analysis of the effect of lipid composition on liposome membrane rigidity[J]. Langmuir, 2016, 32(24): 6 074-6 082.
[12] DONG Xu-yan, KONG Fan-pi, YUAN Gang-you, et al. Optimisation of preparation conditions and properties of phytosterol liposome-encapsulating nattokinase[J]. Natural Product Research, 2012, 26(6): 548-556.
[13] VARSHOSAZ J, JAFARIAN A, SALEHI G, et al. Comparing different sterol containing solid lipid nanoparticles for targeted delivery of quercetin in hepatocellular carcinoma[J]. Journal of Liposome Research, 2014, 24(3): 191-203.
[14] ZHAO Li-sha, TEMELLI F, CURTIS J M, et al. Preparation of liposomes using supercritical carbon dioxide technology: Effects of phospholipids and sterols[J]. Food Research International, 2015, 77: 63-72.
[15] BERRY C J, BIERENBAU M L. Anticholesterolemic edible oil: US, 6277431[P]. 2001-08-21.
[16] 许青青, 金文彬, 苏宝根, 等. 植物甾醇酯的化学合成及其分离研究进展[J]. 中国粮油学报, 2014(3): 120-128.
[17] ALEXANDER M, ACERO LOPEZ A, FANG Ying, et al. Incorporation of phytosterols in soy phospholipids nanoliposomes: Encapsulation efficiency and stability[J]. LWT- Food Science and Technology, 2012, 47(2): 427-436.
[18] WANG Fan-cendy, ACEVEDO N, MARANGONI A G. Encapsulation of phytosterols and phytosterol esters in liposomes made with soy phospholipids by high pressure homogenization[J]. Food & Function, 2017, 8(11): 3 964-3 969.
[19] MENDEZ N, HERRERA V, ZHANG Ling-zhi, et al. Encapsulation of adenovirus serotype 5 in anionic lecithin liposomes using a bead-based immunoprecipitation technique enhances transfection efficiency[J]. Biomaterials, 2014, 35(35): 9 554-9 561.
[20] ZAJICEK J, PEARLMAN J D, MERICKEL M B, et al. High-resolution proton NMR spectra of human arterial plaque[J]. Biochemical & Biophysical Research Communications, 1987, 149(2): 437-442.
[21] SALMON A, HAMILTON J A. Magic-angle spinning and solution 13C nuclear magnetic resonance studies of medium- and long-chain cholesteryl esters in model bilayers[J]. Biochemistry, 1995, 34(49): 16 065-16 073.
[22] 杨贝贝. 甾醇对卵磷脂脂质体性质的影响[D]. 无锡: 江南大学, 2013: 12-13.
[23] KADDAH S, KHREICH N, KADDAH F, et al. Cholesterol modulates the liposome membrane fluidity and permeability for a hydrophilic molecule[J]. Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research Association, 2018, 113: 40-48.
[24] MOSCA M, CEGLIE A, AMBROSONE L. Effect of membrane composition on lipid oxidation in liposomes[J]. Chem Phys Lipids, 2011, 164(2): 158-165.
[25] 杨斌, 刘新铭, 宫泽阳夫, 等. 胆固醇衍生物脂质体的物理稳定性和细胞相容性研究[J]. 现代生物医学进展, 2013, 13(30): 5 801-5 805.
[26] MAR N D, ALEM N A, MONTERO P, et al. Encapsulation of food waste compounds in soy phosphatidylcholine liposomes: Effect of freeze-drying, storage stability and functional aptitude [J]. Journal of Food Engineering, 2018, 223: 132-143.
[27] AZAMBUJA C R L D, SANTOS L G D, RODRIGUES M R, et al. Physico-chemical characterization of asolectin-genistein liposomal system: An approach to analyze its in vitro antioxidant potential and effect in glioma cells viability[J]. Chemistry & Physics of Lipids, 2015, 193: 24-35.
[28] 丁武孝, 齐宪荣, 陈玉武, 等. 琥珀酸胆固醇酯作为脂质体膜稳定剂的研究及其在制备柴胡皂苷-D脂质体中的应用[J]. 药学学报, 2005, 40(7): 623-627.
[29] 张继芬, 唐勤, 莫婷, 等. 姜黄素磷脂载体的表征及肠吸收研究[J]. 中国药学杂志, 2012, 47(21): 1 736-1 740.
[30] PINILLA C M B, THYS R C S, BRANDELLI A. Antifungal properties of phosphatidylcholine-oleic acid liposomes encapsulating garlic against environmental fungal in wheat bread[J]. International Journal of Food Microbiology, 2019, 293: 72-78.
[31] LEE H, CHANG J H. Spherical silica hybrid liposome particles with controlled release of citrus unshiu peel extracts[J]. Materials Chemistry and Physics, 2018, 208: 183-188.