Abstract
A method for rapid determination of aflatoxin B1 in grain based on QD-Infinity immunofluorescence was established with QD-Infinity as fluorescent probe. In order to confirm the accuracy of this method, multiple methods were used to evaluate the uniformity of the test results of AFB1 in grain which was determined by the QD-Infinity immunofluorescence method, high performance liquid chromatography and enzyme association immunoadsorption, respectively. Moreover the repeatability of the method and the difference between stations were investigated. The detection results of the superconducting point-immunofluorescence method for grain samples such as rice and corn were consistent with those of high performance liquid chromatography and enzyme association immunoadsorption, indicated that the method was accurate and reliable. There was no significant difference in the detection results when using two different instruments, which inclined a good repeatability of the method was good too.
Publication Date
1-28-2020
First Page
84
Last Page
87,109
DOI
10.13652/j.issn.1003-5788.2020.01.013
Recommended Citation
Yuan-bin, HU; Yan-xia, XU; Da-neng, WANG; Zhi, MA; Xiao-ying, NI; Yan-ping, TANG; and Yang, YU
(2020)
"Validation of the method for determination of aflatoxin B1 by QD-Infinity immunofluorescence method in grain,"
Food and Machinery: Vol. 36:
Iss.
1, Article 13.
DOI: 10.13652/j.issn.1003-5788.2020.01.013
Available at:
https://www.ifoodmm.cn/journal/vol36/iss1/13
References
[1] 袁航.粮食中主要真菌毒素危害及联合毒性研究进展[J].食品与机械,2019,35(11):223-227.
[2] 陈宁周,颜玉婷,王海波,等.高效液相色谱—串联质谱法测定腐乳中16种真菌毒素[J].食品安全质量检测学报,2019(15):5 169-5 179.
[3] 李梁,唐书泽,杨盼盼,等.流动注射化学发光法在线监测饮用水中赭曲霉毒素A突发污染[J].食品与机械,2019,35(1):86-91,104.
[4] 王光辉,殷勇.基于高光谱融合神经网络的玉米黄曲霉毒素B1和赤霉烯酮含量预测[J].食品与机械,2018,34(11):64-69.
[5] 肖利龙,花锦.高效液相色谱法测定花生酱中黄曲霉毒素B1结果不确定度的评定[J].食品与机械,2018,34(3):79-81,150.
[6] 刘静,王树颖,刘道峰,等.高灵敏黄曲霉毒素B1免疫层析定量检测法的建立[J].食品与机械,2017,33(2):51-55.
[7] 张元杰.ELISA法检测煎饼类食品中黄曲霉毒素含量[J].中国农村卫生,2018(10):12-13.
[8] 潘明飞,李诗洁,郭丹丹,等.间接竞争酶联免疫分析方法检测花生中黄曲霉毒素B1[J].中国食品学报,2019(9):255-261.
[9] 黄轩.食品中黄曲霉毒素B1柱后光化学衍生—高效液相色谱检测法研究[J].食品安全导刊,2018(33):103.
[10] 苏小洁,潘显茂.用免疫亲和柱净化—柱后衍生高效液相色谱荧光法检测山楂干中多种黄曲霉毒素含量的效果观察[J].当代医药论丛,2018(16):189-190.
[11] 卢安根,莫建光,杜寒春,等.酶联免疫法快速检测牛奶及其制品中的黄曲霉毒素B1[J].广西科学院学报,2010(3):306-308.
[12] 杜兵耀,马晨,余雄.胶体金免疫层析法检测谷物中的黄曲霉毒素B1[J].饲料工业,2016(9):58-60.
[13] 李园园,李培武,张奇,等.量子点标记荧光免疫法检测花生中黄曲霉毒素B1[J].中国油料作物学报,2012(4):438-442.
[14] 徐焕焕,余锦露,孙雅静,等.基于CdTe:Zn/ZnS量子点快速检测变形杆菌荧光免疫分析法的建立[J].分析试验室,2019(9):1 065-1 069.