Effects of active peptide components of Eupolyphaga steleophaga on blood rheology, four indicators of blood lipids and blood factor levels in rats with acute blood stasis

Authors

JIANG Shan, School of Pharmacy, Binzhou Medical University, Yantai, Shandong 264003 , China ；School ofPharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250300 , China
WANG Shao-ping, School of Pharmacy, Binzhou Medical University, Yantai, Shandong 264003 , China
DAI Long, School of Pharmacy, Binzhou Medical University, Yantai, Shandong 264003 , China
ZHANG Jia-yu, School of Pharmacy, Binzhou Medical University, Yantai, Shandong 264003 , China
GAO Peng, School ofPharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250300 , China
LIU Zi-han, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488 , China
WANG Yu-qi, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488 , China

Abstract

Pepsin and trypsin complex enzymes were used for biomimetic enzymatic hydrolysis of Eupolyphaga steleophaga. In order to obtain the most active fibrinolytic activity of the soil aphid active peptide component F2, the product was subjected to desalting, ultrafiltration, nanofiltration, DA201-C macroporous adsorption resin, dextran gel G-25 chromatography, reverse high performance liquid chromatography and semi-preparative liquid chromatography. Moreover, its effects on hemorheology index, blood lipids and serum nitric oxide (NO), superoxide dismutase (SOD), tissue plasminogen activator (t-PA) and fibrin (FIB) content of rats with acute blood stasis were also studied. E. ste-leophaga active peptide component F2 could significantly reduce blood viscosity, hematocrit and platelet count (P＜0.05). Compared with the model group, the Eupolyphaga ste-leophaga active peptide component F2 can significantly reduce the content of triglyceride (TG), cholesterol (T-CHO), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) in rat blood (P＜0.05). The levels of NO, SOD and t-PA in the blood of rats were significantly higher than those in the model group, and significant differences in the levels of FIB between the experimental group and the model group (P＜0.05) were also found. The E. ste-leophaga active peptide component F2 prepared by biomimetic enzymatic hydrolysis and purification had the functions of improving blood rheology index, reducing blood lipid levels and changing blood factor content in acute blood stasis model rats.

Publication Date

1-28-2020

First Page

175

Last Page

180

DOI

10.13652/j.issn.1003-5788.2020.01.031

Recommended Citation

Shan, JIANG; Shao-ping, WANG; Long, DAI; Jia-yu, ZHANG; Peng, GAO; Zi-han, LIU; and Yu-qi, WANG (2020) "Effects of active peptide components of Eupolyphaga steleophaga on blood rheology, four indicators of blood lipids and blood factor levels in rats with acute blood stasis," Food and Machinery: Vol. 36: Iss. 1, Article 31.
DOI: 10.13652/j.issn.1003-5788.2020.01.031
Available at: https://www.ifoodmm.cn/journal/vol36/iss1/31

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