Heterologous expression of fourbile salt hydrolases in Lactobacillus fermentum AR497

Authors

YIN Bo-xing, Experimental Farm, Yangzhou University, Yangzhou, Jiangsu 225009 , China ；Jiangsu DairyBioengineering Technology Research Center, Yangzhou, Jiangsu 225004 , China ；Yangda Kangyuan Dairy Co., Ltd., Yangzhou, Jiangsu 225004 , China
SHI Jun-kang, School of MedicalInstrument and Food Engineering, University of Shanghai for Science and Technology,Shanghai 200093 , China ；Shanghai Research Center of Food Microbiology, Shanghai 200093 , China
KONG Ling-hui, School of MedicalInstrument and Food Engineering, University of Shanghai for Science and Technology,Shanghai 200093 , China ；Shanghai Research Center of Food Microbiology, Shanghai 200093 , China
YANG Ren-qing, Experimental Farm, Yangzhou University, Yangzhou, Jiangsu 225009 , China ；Jiangsu DairyBioengineering Technology Research Center, Yangzhou, Jiangsu 225004 , China ；Yangda Kangyuan Dairy Co., Ltd., Yangzhou, Jiangsu 225004 , China
AI Lian-zhong, School of MedicalInstrument and Food Engineering, University of Shanghai for Science and Technology,Shanghai 200093 , China ；Shanghai Research Center of Food Microbiology, Shanghai 200093 , China
XIONG Zhi-qiang, School of MedicalInstrument and Food Engineering, University of Shanghai for Science and Technology,Shanghai 200093 , China ；Shanghai Research Center of Food Microbiology, Shanghai 200093 , China

Abstract

In order to improve bile salt tolerance in L. fermentum, four BSH genes (BSH1,BSH2,BSH3, and BSH4) from L. plantarum AR113 were transformed into L. fermentum AR497. The result showed that heterologous expression of these four BSHs can improve the bile salt tolerance of L. fermentum. At the concentration of 2.0 mg/mL sodium glycodeoxycholate, engineered L. fermentumwith BSH2 expression had the lowest lethality, while other strains were completely inhibited. BSH activity showed that BSH2 expressing strain had the highest enzyme activity with 57.74 U/mL, which was 2.88 times higher than that of the control strain harboring empty plasmid.

Publication Date

1-28-2020

First Page

56

Last Page

60

DOI

10.13652/j.issn.1003-5788.2020.01.008

Recommended Citation

Bo-xing, YIN; Jun-kang, SHI; Ling-hui, KONG; Ren-qing, YANG; Lian-zhong, AI; and Zhi-qiang, XIONG (2020) "Heterologous expression of fourbile salt hydrolases in Lactobacillus fermentum AR497," Food and Machinery: Vol. 36: Iss. 1, Article 8.
DOI: 10.13652/j.issn.1003-5788.2020.01.008
Available at: https://www.ifoodmm.cn/journal/vol36/iss1/8

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