Optimization of ultrasonic-assisted extraction for polyphenols from perilla leaves by polyethylene glycol and its antioxidant activity

Authors

Qiao-mei RU, Department of Health and Agriculture, Hangzhou Wanxiang Polytechnic, Hangzhou, Zhejiang 310023 , China
Guo-ping REN, Department of Health and Agriculture, Hangzhou Wanxiang Polytechnic, Hangzhou, Zhejiang 310023 , China
Qiong HU, Department of Health and Agriculture, Hangzhou Wanxiang Polytechnic, Hangzhou, Zhejiang 310023 , China

Abstract

On the basis of single factor experiment, the concentration of polyethylene glycol, liquid/solid ratio, ultrasonic power and extraction time were optimized by Box-Behnken response surface analysis. Result: The results showed that the optimal extraction conditions were concentration of polyethylene glycol 68%, liquid/solid ratio 46∶1 (mL/g), ultrasonic power 157 W and extraction time 67 min. Under these conditions, the yield of polyphenols from perilla leaves reached 18.38 mg GAE/g·DW, while the predicted value was 18.21 mg GAE/g·DW, and the accuracy of the model reached 99.08%. Polyphenols from perilla leaves exhibited good antioxidant activity in a concentration-dependent manner. The IC₅₀ of polyphenols to hydroxyl radical (·OH), superoxide anion radical (O ·), 1,1-diphenyl-2-trinitrophenylhydrazine radical (DPPH·) and reducing power were 60.21, 73.44, 70.81 and 37.52 μg/mL, respectively. However, the antioxidant activity of polyphenols extracted from perilla leaves was weaker than that of ascorbic acid (V_C).

Publication Date

2-18-2023

First Page

136

Last Page

142

DOI

10.13652/j.issn.1003-5788.2020.10.027

Recommended Citation

RU, Qiao-mei; REN, Guo-ping; and HU, Qiong (2023) "Optimization of ultrasonic-assisted extraction for polyphenols from perilla leaves by polyethylene glycol and its antioxidant activity," Food and Machinery: Vol. 36: Iss. 10, Article 27.
DOI: 10.13652/j.issn.1003-5788.2020.10.027
Available at: https://www.ifoodmm.cn/journal/vol36/iss10/27

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