Abstract
Two strains of fungi producing β-glucosidase were screened and identified from Martianus dermestoides. The conversion of rare ginsenosides and fermentation quality were improved by combined fermentation of the two strains. The dynamic fermentation process was studied, and the mechanism of ginsenoside transformation was clarified. Screening was performed by MRS (aesculin, ferric citrate) medium. Two fungi producing β-glucosidase were identified by 18S rRNA gene amplification. The strains were fermented with total ginsenoside, the fermentation products were analyzed by LC-MS/MS. Two fungi producing β-glucosidase were identified as genus Chaetomium and Aspergillus respectively. The fermentation products of WY1 reacted with total ginsenosides were Rd and Rh1. The fermentation products of WY2 reacted with total ginsenosides were Rd, Rh1, Rg3 and compound K. The conversion rates of Rh1, Rg3 and compound K were improved by WY1/ WY2 combined fermentation. The transformation path was Re→Rg1→Rh1; Rf→Rh1; Rb1/Rb2/Rc→Rd→Rg3/compound K. Protopanaxadiol-type ginsenosides were more easily converted than protopanaxatriol-type ginsenosides and most of the products are Rg3.
Publication Date
2-18-2023
First Page
16
Last Page
22,90
DOI
10.13652/j.issn.1003-5788.2020.11.003
Recommended Citation
FANG, Liu; WANG, Yan-cheng; JI, Wen-xiu; and DONG, Wei-wei
(2023)
"Screening and identification of two strains of β-glucosidase producing symbiotic fungi in Martianus dermestoides and the mechanism of combined transformation of ginsenoside,"
Food and Machinery: Vol. 36:
Iss.
11, Article 3.
DOI: 10.13652/j.issn.1003-5788.2020.11.003
Available at:
https://www.ifoodmm.cn/journal/vol36/iss11/3
References
[1] 逄世峰,李亚丽,许世泉,等.人参不同部位人参皂苷类成分研究[J].人参研究,2015,27(1):5-8.
[2] 吴晓民,赵丹,朱艳萍,等.人参多糖的药理作用与临床研究进展[J].人参研究,2016,28(5):40-46.
[3] JIN Xin,CHE Dao-biao,ZHANG Zhen-hai,et al.Ginseng consumption and risk of cancer:A meta-analysis[J].Journal of Ginseng Research,2016,40(3):269-277.
[4] KIM U,PARK M H,KIM D H,et al.Metabolite profiling of ginsenoside Re in rat urine and faeces after oral administration[J].Food Chem,2013,136(3/4):1 364-1 369.
[5] SUN J,WEI W,GUO Y,et al.Pharmacokinetic study of ginsenoside Rc and simultaneous determination of its metabolites in rats using RRLC-Q-TOF-MS[J].J Pharm Biomed Anal,2014,88:16-21.
[6] 史得君,严欢,崔清美,等.人参茎叶提取物对Ⅰ型糖尿病小鼠模型的影响[J].食品与机械,2017,33(7):165-169.
[7] 王莹.长白山虫草真菌转化稀有人参皂苷菌株的筛选、鉴定及转化条件的优化[D].长春:吉林化工学院,2019:5.
[8] 孙广仁,赵闻琦.多菌种发酵人参酒及皂苷转化分析[J].食品科学,2011,32(23):234-239.
[9] 蔡爽.人参术苓酵素的制备及改善肠胃功能研究[D].长春:吉林大学,2006:8.
[10] 韩铭鑫,李方彤,张琰,等.稀有原人参二醇型皂苷的人肠道菌群生物转化[J].高等学校化学学报,2019,40(7):1 390-1 396.
[11] 卲淇,陈贺,尹成日.乳酸菌对人参皂苷Rb1的生物转化研究[J].延边大学学报(自然科学版),2016,42(2):177-180.
[12] 许春春,于渤浩,王红蕾,等.重组嗜热β-葡萄糖苷酶转化稀有人参皂苷Rd和CK[J].高等学校化学学报,2016,37(2):281-289.
[13] 杜娇,姜淑喆,未建华.白蚁及其共生菌来源的4种木质纤维素酶的共表达[J].生物工程学报,2019,35(2):79-88.
[14] WANG Wei-nan,YAN Bing-xiong,XU Wen-di,et al.Highly selective bioconversion of ginsenoside Rb1 to compound K by the mycelium of cordyceps sinensis under optimized conditions[J].Molecules,2015,20(10):19 291-19 309.
[15] 汤蕾,余晓斌,郝学财,等.响应面法优化虫草液态发酵培养基[J].食品与机械,2005,21(6):41-43.
[16] 王长文,房柳,梁运江,等.基于高通量测序的不同虫态洋虫内共生微生物群落分析[J].微生物学报,2019,59(9):1 705-1 713.
[17] DONG Wei-wei,XUAN Fang-ling,ZHONG Fei-liang,et al.Comparative analysis of the rats' gut microbiota composition in animals with different ginsenosides metabolizing activity[J].Journal of Agricultural and Food Chemistry,2017,65(2):327-337.
[18] 刘文静,程晗,陈崇艺,等.产β-葡萄糖苷酶菌株的筛选及产酶条件优化[J].食品与发酵工业,2019,45(23):43-49.
[19] 田蕾.三七双向发酵技术的研究[D].大连:大连工业大学,2015:5.
[20] 马宗敏,段绪红,秦梦,等.微生物发酵技术在中药苷类生物转化中的应用进展[J].世界科学技术:中医药现代化,2017,19(5):858-864.
[21] 左明星,周彦伶,许言超,等.烟曲霉GZWMJZ-152茶粕发酵代谢产物[J].菌物学报,2019,38(2):264-271.
[22] 张欣宇.复合益生菌微生态添加剂的培养条件优化及功效评价[D].长春:吉林大学,2015:5.