Separation and purification of polysaccharides from liquid fermentation product by Cordyceps tenuipes

Authors

Xiao-ai WANG, College of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guizhou,Guiyang 550025 , China ；Center for Mycomedicine Research, Guizhou University ofTraditional Chinese Medicine, Guizhou, Guiyang 550025 , China
Jian-dong LIANG, College of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guizhou,Guiyang 550025 , China ；Center for Mycomedicine Research, Guizhou University ofTraditional Chinese Medicine, Guizhou, Guiyang 550025 , China
Xiao-jiao GAO, College of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guizhou,Guiyang 550025 , China
Yuan-jun CAI, College of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guizhou,Guiyang 550025 , China
Yu-meng QIN, College of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guizhou,Guiyang 550025 , China
Wei-yi TIAN, College of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guizhou,Guiyang 550025 , China ；Center for Mycomedicine Research, Guizhou University ofTraditional Chinese Medicine, Guizhou, Guiyang 550025 , China

Abstract

Using Box-Behnken central combination test design and single factor test were used to optimize the fermentation process of cordyceps polysaccharide, water extraction and alcohol precipitation process and deproteinization process. The results showed that the optimum fermentation conditions of Cordyceps tenuipes were glucose 33.3 g/L, peptone 29.2 g/L, inoculation amount 3.9%. The optimum extraction conditions of Exopolysaccharides were concentration, 3 times ethanol, pH 6.0 and precipitation for 24 h. The optimum extraction conditions were 1∶40 (g/mL), extraction time 90 min, extraction times 2 times, and extraction temperature 80 ℃. In the purification of polysaccharides, the optimum deproteinization techniques of Sevage were as follows: the volume ratio of chloroform to n-butanol was 4∶1, the volume ratio of Sevage reagent to fermentation broth was 1∶2, the shaking time was 15 min, and the deproteinization times was 3 times. After optimization, the yield of extracellular polysaccharide increased by 37.89%, that of intracellular polysaccharide increased by 24.59%, and the protein removal rate was more than 75% after purification.

Publication Date

2-18-2023

First Page

158

Last Page

164,216

DOI

10.13652/j.issn.1003-5788.2020.11.031

Recommended Citation

WANG, Xiao-ai; LIANG, Jian-dong; GAO, Xiao-jiao; CAI, Yuan-jun; QIN, Yu-meng; and TIAN, Wei-yi (2023) "Separation and purification of polysaccharides from liquid fermentation product by Cordyceps tenuipes," Food and Machinery: Vol. 36: Iss. 11, Article 31.
DOI: 10.13652/j.issn.1003-5788.2020.11.031
Available at: https://www.ifoodmm.cn/journal/vol36/iss11/31

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