An UHPLC-MS/MS method for rapid analysis of tea for the potential presence of illegally added flavorings

Authors

Zhi-sen LIANG, Guangzhou Inspection and Testing Certification Group Company Limited, Guangzhou, Guangdong 511447 , China ；National Quality Testing Center for Processed Food ﹝Guangdong﹞, Guangzhou, Guangdong 511447 , China
Yu-zhen CHEN, Guangzhou Inspection and Testing Certification Group Company Limited, Guangzhou, Guangdong 511447 , China ；National Quality Testing Center for Processed Food ﹝Guangdong﹞, Guangzhou, Guangdong 511447 , China
Lang-jun ZHOU, Guangzhou Inspection and Testing Certification Group Company Limited, Guangzhou, Guangdong 511447 , China ；National Quality Testing Center for Processed Food ﹝Guangdong﹞, Guangzhou, Guangdong 511447 , China

Abstract

A qualitative and quantitative method for simultaneous detection of 7 potential presence of illegally-added flavorings, including trans-anethole, coumarin, estragole, lawsone, methyleugenol, pulegone and trans-cinnamaldehyde in tea samples. Tea samples were extracted with acetonitrile-water served as a mixed solvent, subjected to whirlpool oscillation and then diluted properly. After that, anhydrous sodium sulfate, primary secondary amine (PSA) and octadecylsiyl silica gel (C₁₈) were added to the solvent for purification. The centrifugation, the supernatant was filtered over a membrane and separated on a Kinetex C₁₈ column. These 7 target compounds were determined by UHPLC-MS/MS in MRM mode, and quantified utilizing a matrix-matched external standard method. The results showed a good linearity from 0.8~1 600.0 μg/L and the value of the corresponding correlation coefficients (R2) were all larger than 0.999. The values of limit of detection were 0.02~0.03 mg/kg, and those of limit of quantification were 0.05~0.10 mg/kg, the average recoveries were 82.5%~102.5%, and the intra-day and inter-day RSD values were below 16% and 18%, respectively. A survey of 100 commercial tea samples was conducted, showing that trans-anethole, coumarin and estragole were present in green and black tea with maximum concentrations of 65.0, 2.0 and 72.2 mg/kg, respectively.

Publication Date

2-18-2023

First Page

51

Last Page

57

DOI

10.13652/j.issn.1003-5788.2020.11.009

Recommended Citation

LIANG, Zhi-sen; CHEN, Yu-zhen; and ZHOU, Lang-jun (2023) "An UHPLC-MS/MS method for rapid analysis of tea for the potential presence of illegally added flavorings," Food and Machinery: Vol. 36: Iss. 11, Article 9.
DOI: 10.13652/j.issn.1003-5788.2020.11.009
Available at: https://www.ifoodmm.cn/journal/vol36/iss11/9

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