Abstract
The aim of the present study is to investigate the potential anti-atherogenic effect of 4-hydroxyphenylacetic acid (4-HPAA), a major microbiota-derived metabolite of polyphenols, on macrophagepolarization and the formation of macrophage derived-foam cells as well as the underlying molecular mechanisms. 4-HPAA treatment could-significantly decrease the secretion and mRNA levels of TNF-α, IL-6 and IL-1β of M1 macrophages induced by lipopolysaccharide (LPS) and interferon-γ (IFN-γ),indicating its ability to inhibit the polarization of macrophage cell into pro-inflammatory M1-typemacrophage. The results of oil red O staining revealedthat 4-HPAA significantly inhibited theaccumulation of lipid droplets in a dose-dependent manner compared with the model cells. In addition, 4-HPAA significantly decreased the content of intracellular cholesterol esters.RT-PCR results showed that compared with the model group, 4-HPAA significantly up-regulated ABCG1 and down-regulated CD36 gene expression level (P ＜ 0.05), which was associated with macrophage cholesterol efflux. In conclusion, the results showed that the potential role of 4-HPAA in preventing M1 macrophage polarization and foam cell formation, which may play an important role in the anti-atherogenic effect of polyphenols.
Publication Date
2-18-2023
First Page
2
Last Page
6,89
DOI
10.13652/j.issn.1003-5788.2020.12.001
Recommended Citation
YANG, YU-zhe; LI, Tong-yun; LI, Wu; and YANG, Rui-li
(2023)
"Effect of 4-Hydroxyphenylacetic acid on M1 macrophage polarization and the formation of macrophage-derived foam cells,"
Food and Machinery: Vol. 36:
Iss.
12, Article 1.
DOI: 10.13652/j.issn.1003-5788.2020.12.001
Available at:
https://www.ifoodmm.cn/journal/vol36/iss12/1
References
[1] YUAN Yuan,LI Peng,YE Jing.Lipid homeostasis and the formation of macrophage-derived foam cells in atherosclerosis[J].Protein & Cell,2012,3(3):173-181.
[2] ZALARR D M,POP C,BUZDUGAN E,et al.The atherosclerosis-inflammation relationship-A pathophysiological approach[J].Framacia,2019,67(6):941-947.
[3] YANG Sai,YUAN Hou-qin,HAO Ya-meng,et al.Macrophage polarization in atherosclerosis[J].Clinica Chimica Acta,2020,501:142-146.
[4] 白瑞娜,郗瑞席,冯志博,等.巨噬细胞与动脉粥样硬化-亚型及功能[J].中国循环杂志,2014,29(5):393-395.
[5] 刘俊田.动脉粥样硬化发病的炎症机制的研究进展[J].西安交通大学学报(医学版),2015,36(2):141-152.
[6] MAGUIRE E M,PEARCE S W,XIAO Qing-zhong.Foam cell formation:A new target for fighting atherosclerosis and cardiovascular disease[J].Vascular Pharmacology,2019,112(S1):54-71.
[7] KOTLA S,SINGH N K,RAO GN.ROS via BTK-p300-STAT1-PPAR gamma signaling activation mediates cholesterol crystals-induced CD36 expression and foam cell formation[J].Redox Biology,2017,11:350-364.
[8] DERGUNOY A D,SAVUSHKIN E V,DERGUNOVA L V,et al.Significance of cholesterol-binding motifs in ABCA1,ABCG1,and SR-B1 structure[J].Journal of Membrane Biology,2018,252(1):1-20.
[9] RUBIC T,LORENZ R L.Downregulated CD36 and ox-LDL uptake and stimulated ABCA1/G1 and cholesterol efflux as anti-atherosclerotic mechanisms of interleukin-10[J].Cardiovascular Research,2006,69(2):527-535.
[10] BAHRAMSOLTAN R,EBRAHIMI F,FARZAEIM H.Dietary polyphenols for atherosclerosis:A comprehensive review and future perspectives[J].Critical Reviews in Food Science and Nutrition,2019,59(1):114-132.
[11] CHOI Dong-young,LEE Young-jung,HONG Jin-tae,et al.Antioxidant properties of natural polyphenols and their therapeutic potentials for Alzheimer's disease[J].Brain Research Bulletin,2012,87(2/3):144-153.
[12] SANTHAKUMAR A B,BATTINO M,ALVAREZ-SUAREZ J M.Dietary polyphenols:Structures,bioavailability and protective effects against atherosclerosis[J].Food and Chemical Toxicology,2018,113:49-65.
[13] TENG Hui,CHEN Lei.Polyphenols and bioavailability:An update[J].Critical Reviews in Food Science and Nutrition,2019,59(13):2 040-2 051.
[14] PIWOWARSKII J P,KISS A K,GRANICA S.Urolithins,gut microbiota-derived metabolites of ellagitannins,inhibit LPS-induced inflammation in RAW 264.7 murine macrophages[J].Molecular Nutrition & Food Research,2015,59(11):2 168-2 177.
[15] GUADAMURO L,DOHRMANN A B,TEBBE C C.Bacterial communities and metabolic activity of faecal cultures from equol producer and non-producer menopausal women under treatment with soy isoflavones[J].BMC Microbiology,2017,93:17.
[16] DEPREZ S,BREZILLON C,RABOT S.Polymeric proanthocyanidins are catabolized by human colonic microflora into low-molecular-weight phenolic acids[J].Journal of Nutrition,2000,130(11):2 733-2 738.
[17] MORADI-AFRAPOLI F,OUFIR M,WALTER F R,et al.Validation of UHPLC-MS/MS methods for the determination of kaempferol and its metabolite 4-hydroxyphenyl acetic acid,and application to in vitro blood-brain barrier and intestinal drug permeability studies[J].Journal of Pharmaceutical and Biomedical Analysis,2016,128:264-274.
[18] ZABELA V,SAMPATH C,OUFIR M,et al.Pharmacokinetics of dietary kaempferol and its metabolite 4-hydroxyphenylacetic acid in rats[J].Fitoterapia,2016,115:189-197.
[19] 邢贝贝,张亭亭,赵强,等.高压微射流处理对米谷蛋白热聚集体性质的影响[J].食品科学,2019,40(3):109-115.
[20] HU Chun,XIONG Zhou-yi,XIONG Han-guo,et al.Effects of dynamic high-pressure microfluidization treatment on the functional and structural properties of potato protein isolate and its complex with chitosan[J].Food Research International,2020,DOI:10.1016/j.foodres.2020.109868.
[21] LI Su-yun,YANG Xue,ZHANG Yan-yan,et al.Effects of ultrasound and ultrasound assisted alkaline pretreatments on the enzymolysis and structural characteristics of rice protein[J].Ultrason Sonochem,2016,31:20-28.
[22] WANG Tao,WANG Li,WANG Ren,et al.Effects of freeze-milling on the physicochemical properties of rice protein isolates[J].LWT-Food Science and Technology,2016,65:832-839.
[23] 胥伟,代钰,王宏勋,等.冷冻处理对蛋清液起泡性与凝胶性的影响[J].食品工业,2019,40(5):96-98.
[24] 石嘉怿,张太,梁富强,等.大米谷蛋白储藏过程中结构与功能性质变化的研究[J/OL].食品工业科技.[2020-12-12].https://doi.org/10.13386/j.issn1002-0306.2020100061.