Abstract
In order to study the biological function of GmWRKY86 gene in soybean on development and resistance against abiotic stress, PCR products of its opening reading frame, ORF) were used to clone GmWRKY86 gene, and the gene expressions in different tissues were detected by using real-time fluorescence quantitative PCR (qRT-PCR). Sequence analysis showed that the GmWRKY86 gene can encode 523 amino acids with an isoelectric point of 8.15, a molecular weight predicted to be 56.82 kDa, and an open reading frame of 1 572 bp. GmWRKY86 protein has two highly conserved domains, and the GmWRKY86 gene contains three introns and four exons. Phylogenetic tree analysis indicated that the GmWRKY86 protein belongs to the same branch as the FvWRKY42 (Fragaria vesca) and VvWRKY2 (Vitis vinifera). qRT-PCR analysis showed that GmWRKY86 gene was expressed in all tissues examined, with the highest expression in flowers and the lowest expression in seeds, indicating that GmWRKY86 gene involves in the development of soybean flower organs.
Publication Date
2-28-2020
First Page
69
Last Page
72
DOI
10.13652/j.issn.1003-5788.2020.02.012
Recommended Citation
Jing, WANG; Jing, FU; and Xiao-wei, LI
(2020)
"Cloning and expression analysis of GmWRKY86 in soybean,"
Food and Machinery: Vol. 36:
Iss.
2, Article 12.
DOI: 10.13652/j.issn.1003-5788.2020.02.012
Available at:
https://www.ifoodmm.cn/journal/vol36/iss2/12
References
[1] ISHIGURO S,NAKAMURA K.Characterization of a cDNA encoding a novel DNA-binding protein,SPF1,that recognizes SP8 sequences in the 5′upstream regions of genes coding for sporamin and β-amylase from sweet potato[J].Molecular Genetics and Genomics,1994,244(6):563-571.
[2] EULGEM T,RUSHTON P J,ROBATZEK S,et al.The WRKY superfamily of plant transcription factors[J].Trends in Plant Science,2000,5(5):199-206.
[3] RUSHTON P J,SOMSSICH I E,RINGLER P,et al.WRKY transcription factors[J].Trends in Plant Science,2010,15(5):247-258.
[4] EULGEM T,SOMSSICH I E.Networks of WRKY transcription factors in defense signaling[J].Current Opinion Plant Biology,2007,10(4):366-371.
[5] ISHIHAMA N,YOSHIOKA H.Post-translational regulation of WRKY transcription factors in plant immunity[J].Current Opinion Plant Biology,2012,15(4):431-437.
[6] LAGACÉ M,MATTON D P.Characterization of a WRKY transcription factor expressed in late torpedo-stage embryos of Solanum chacoense[J].Planta,2004,219(1):185-189.
[7] HINDERHOFER K,ZENTGRAF U.Identification of a transcription factor specifically expressed at the onset of leaf senescence[J].Planta,2001,213(3):469-473.
[8] PNUELI L,HALLAK-HERR E,ROZENBERG M,et al.Molecular and biochemical mechanisms associated with dormancy and drought tolerance in the desert legume Retama raetam[J].Plant Journal,2012,31(3):319-330.
[9] JOHNSON C S,KOLEVSKI B,SMYTH D R.TRANSPARENT TESTA GLABRA2,a trichome and seed coat development gene of Arabidopsis,encodes a WRKY transcription factor[J].Plant Cell,2002,14(6):1 359-1 375.
[10] LUO Ming,DENNIS E S,BERGER F,et al.MINISEED3(MINI3),a WRKY family gene,and HAIKU2(IKU2),a leucine-rich repeat(LRR)KINASE gene,are regulators of seed size in Arabidopsis[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(48):17 531-17 536.
[11] ZHANG Zhong-lin,XIE Zhen,ZOU Xiao-lu,et al.A rice WRKY gene encodes a transcriptional repressor of the gibberellin signaling pathway in aleurone cells[J].Plant Physiology,2004,134(4):1 500-1 513.
[12] RUSHTON D L,TRIPATHI P,RABARA R C,et al.WRKY transcription factors:Key components in abscisic acid signaling[J].Plant Biotechnology Journal,2012,10(1):2-11.
[13] DU Li-qun,CHEN Zhi-xiang.Identification of genes encoding receptor-like protein kinases as possible targets of pathogen-and salicylic acid-induced WRKY DNA-binding proteins in Arabidopsis[J].Plant Journal,2000,24(6):837-847.
[14] CHEN Li-gang,SONG Yu,LI Shu-jia,et al.The role of WRKY transcription factors in plant abiotic stresses[J].Biochimica Biophysica Acta,2012,1 819(2):120-128.
[15] BABITHA K C,RAMU S V,PRUTHVI V,et al.Co-expression of AtbHLH17 and AtWRKY28 confers resistance to abiotic stress in Arabidopsis[J].Transgenic Research,2013,22(2):327-341.
[16] BO Chen,CHEN Hao-wei,LUO Guo-wei,et al.Maize WRKY114 gene negatively regulates salt-stress tolerance in transgenic rice[J].Plant Cell Report,2019,DOI:10.1007/s00299-019-02481-3.
[17] PILLAI S E,KUMAR C,PATEL H K,et al.Overexpression of a cell wall damage induced transcription factor,OsWRKY42,leads to enhanced callose deposition and tolerance to salt stress but does not enhance tolerance to bacterial infection[J].BMC Plant Biology,2018,18(1):177.
[18] 沈晓蕾,李向红,俞健,等.大豆分离蛋白、木薯淀粉与转谷氨酰胺酶组合对鲢鱼鱼糜凝胶品质的影响[J].食品与机械,2019,35(9):26-31.
[19] SONG Hui,WANG Peng-fei,HOU Lei,et al.Global analysis of WRKY genes and their response to dehydration and salt stress in soybean[J].Frontiers in Plant Science,2016,7:9.
[20] YU Yan-chong,WANG Nan,HU Rui-bo,et al.Genome-wide identification of soybean WRKY transcription factors in response to salt stress[J].Springerplus,2016,5(1):920.
[21] WEI Wei,CUI Meng-yuan,HU Yang,et al.Ectopic expression of FvWRKY42,a WRKY transcription factor from the diploid woodland strawberry(Fragaria vesca),enhances resistance to powdery mildew,improves osmotic stress resistance,and increases abscisic acid sensitivity in Arabidopsis[J].Plant Science,2018,275:60-74.
[22] MZID Rim,ZORRIG Walid,BEN AYED Rayda,et al.The grapevine VvWRKY2 gene enhances salt and osmotic stress tolerance in transgenic Nicotiana tabacum[J].3 Biotech,2018,8(6):277.