Inhibitory effect of exo-polysaccharide from Rhodotorula mucilaginosa CICC 33013 on hepatocellular carcinoma cell activity

Authors

MA Wen-jin, Gansu Institute of Light Industry Limited Liability Company, Lanzhou, Gansu 730000 , China
LI Mei-lin, Gansu Institute of Light Industry Limited Liability Company, Lanzhou, Gansu 730000 , China
WANG Bo, Gansu Institute of Light Industry Limited Liability Company, Lanzhou, Gansu 730000 , China
ZHOU Yan-bing, Gansu Institute of Light Industry Limited Liability Company, Lanzhou, Gansu 730000 , China
PENG Tao, Gansu Institute of Light Industry Limited Liability Company, Lanzhou, Gansu 730000 , China
ZHANG Yong-xian, Gansu Institute of Light Industry Limited Liability Company, Lanzhou, Gansu 730000 , China
YU Chang-qing, Gansu Institute of Light Industry Limited Liability Company, Lanzhou, Gansu 730000 , China
BAN Sheng-hua, Gansu Institute of Light Industry Limited Liability Company, Lanzhou, Gansu 730000 , China

Abstract

The anti-tumor activity of the exo-polysaccharide component REPS2-A metabolized by Rhodotorula mucilaginosa CICC 33013 was studied. The exo-polysaccharide component REPS2-A was inhibited by tetramethylazole blue (MTT) method in 10 common cancer cells including blood cancer cell K562, gastric cancer cell BGC823, gastric cancer cell SGC7901, gastric cancer cell MKN28, liver cancer cell HepG2, BEL7402, Hep3B, pancreatic cancer cell HS66T, breast cancer cell SKBR3, and cervical cancer cell HeLa. The results showed that the exo-polysaccharide component REPS2-A had a good inhibitory effect on HepG2 cells. By studying the optimal concentration and time effect of exo-polysaccharide component REPS2-A on HepG2 cells, the growth inhibition and its mechanism of exo-polysaccharide component REPS2-A on HepG2 cells were investigated. The results showed that when the concentration of exo-polysaccharide component REPS2-A was 1 mg/mL, the inhibition rate of HepG2 in liver cancer cells was higher than that in IC50, and the inhibition effect was significantly better than that in other concentrations. The spontaneous apoptosis rate of HepG2 was 0.40%. When the concentration of exo-polysaccharide component REPS2-A was 1 mg/mL, the apoptosis rate of HepG2 was 77.70%, 88.18% and 97.08% after 24, 48 and 72 h treatment, respectively. Exo-polysaccharide component REPS2-A could effectively inhibit the proliferation of liver cancer cells, cause HepG2 block in the G1/S phase, and induce HepG2 apoptosis in liver cancer cells with dose effect.

Publication Date

2-28-2020

First Page

159

Last Page

164

DOI

10.13652/j.issn.1003-5788.2020.02.030

Recommended Citation

Wen-jin, MA; Mei-lin, LI; Bo, WANG; Yan-bing, ZHOU; Tao, PENG; Yong-xian, ZHANG; Chang-qing, YU; and Sheng-hua, BAN (2020) "Inhibitory effect of exo-polysaccharide from Rhodotorula mucilaginosa CICC 33013 on hepatocellular carcinoma cell activity," Food and Machinery: Vol. 36: Iss. 2, Article 30.
DOI: 10.13652/j.issn.1003-5788.2020.02.030
Available at: https://www.ifoodmm.cn/journal/vol36/iss2/30

References

[1] 杨铿,杨莺莺,李卓佳,等.胶红酵母(Rhodotorula muci-laginosa)营养成分分析[J].广东农业科学,2014,41(5):146-149.
[2] RAVELLA S R,QUIÑONES T S,RETTER A,et al.Exo-polysaccharide(EPS)production by a novel strain of yeast-like fungusAureobasidium pullulans[J].Carbohydrate Polymers,2010,82(3):728-732.
[3] 孙佳静,李貌,孙志洪,等.胶红酵母产类胡萝卜素固态发酵工艺[J].中国农业科学,2018,51(10):1 982-1 994.
[4] 俞国伟,魏春,张艳丽,等.胶红酵母利用番茄渣和豆粕为原料发酵生产类胡萝卜素的研究[J].生物学杂志,2014(4):55-59.
[5] 梁金钟,梅剑秋,王翼雪.胶红酵母菌降解毒死蜱发酵工艺研究[J].农产品加工,2017(1):45-50.
[6] 管志荣,朱研,童志霞,等.骆驼刺青贮中胶红酵母菌分离鉴定和菌株特性研究[J].畜牧与饲料科学,2014(8):25-27.
[7] PAROLIS L A,PAROLIS H,KENNE L,et al.The extracellular polysaccharide of Pichia(Hansenula)holstii NRRL Y-2448:The phosphorylated side chains[J].Carbohydrate Research,1998,309(1):65-77.
[8] HASHIMOTO K,OKAWA Y,SUZUKI K,et al.Antitumor activity of acidic mannan fraction from bakers’ yeast[J].Journal of Pharmacobio-dynamics,1983,6(9):668-675.
[9] FREITAS F,ALVES V D,REISM A M.Advances in bacterial exopolysaccharides:From production to biotechnological applications[J].Trends in Biotechnology,2011,29(8):388-398.
[10] 包怡红,梁雪,李锐达,等.产胞外多糖酵母菌株的筛选鉴定及发酵产糖[J].微生物学报,2010,50(2):278-283.
[11] MA Wen-Jin,CHEN Xue-feng,WANG Bo,et al.Characterization,antioxidativity,and anti-carcinoma activity of exopolysaccharide extract from Rhodotorula mucilaginosa CICC 33013[J].Carbohydrate Polymers,2018,181(1):768-777.
[12] KHALIKOVA T A,ZHANAEVA S Y,KOROLENKO T A,et al.Regulation of activity of cathepsins B,L,and D in murine lymphosarcoma model at a combined treatment with cyclophosphamide and yeast polysaccharide[J].Cancer Letters,2005,223(1):77-83.
[13] KRIZKOVÁ L,DURACKOV Z,SANDULA J,et al.Antioxidative and antimutagenic activity of yeast cell wall mannansin vitro[J].Mutation Research/Genetic Toxicology & Environmental Mutagenesis,2001,497(1/2):213-222.
[14] 孙建新,朱虎虎,肖辉,等.4种新疆植物提取物对人肝癌细胞生长抑制作用的研究[J].新疆医科大学学报,2008,31(7):828-830.
[15] LIN Yu-ching,LU Li-ting,CHEN Hsin-yi,et al.SCP phosphatases suppress renal cell carcinoma by stabilizing PML and inhibiting mTOR/HIF signaling[J].Cancer Research,2014,74(23):6 935-6 946.
[16] WANG Yue,WANG Wen-liang,XIE Wen-li,et al.Puerarin stimulates proliferation and differentiation and protects against cell death in human osteoblastic MG-63 cells via ER-dependent MEK/ERK and PI3K/Akt activation[J].Phytomedicine,2013,20(10):787-796.
[17] 刘婷,傅颖媛.丰城鸡血藤H-103树脂提纯物体外抗肿瘤作用研究[J].时珍国医国药,2010,21(6):1 537-1 539.
[18] 王惠国,李雨桐,蔡珂,等.青蒿素抑制人肝癌细胞Huh7和SMMC-7721增殖及其机制[J].华西药学杂志,2019(6):1-4.
[19] 张华,王振宇.黑木耳多糖的羧甲基化及其对肝癌细胞HePG2的抑制作用[J].食品与机械,2011,27(3):42-44.