Rapid detection of Alternaria monomethyl ether in wheat by high performance liquid chromatography

Authors

XU Qun-ying, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, Hubei 430023 , China
ZHAI Yu-heng, National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi,Jiangsu 214122 , China

Abstract

In this study, a high-performance liquid chromatography (HPLC) method for the efficient detection of alternariolmethyl ether (AME) in wheat was developed. The sample was extracted with acetonitrile/ methanol/ water (volume ratio 45/10/45), shaken at 30 ℃ for 30 minutes, and purified by Bond Elut Plexa solid-phase extraction cartridge. Then the sample was detected with a Variable wavelength detector. In this method, the limit of detection (LOD) of AME was 2.52 μg/kg, and the limit of quantification (LOQ) was 8.40 μg/kg, and the linear range was 0.25~2.00 μg/mL. The concentration of AME recovery was 80.1%~88.3%, the intra-day precision and inter-day precision were 2.13%~6.59% and 3.57%~8.43%, respectively.

Publication Date

3-28-2020

First Page

60

Last Page

62,118

DOI

10.13652/j.issn.1003-5788.2020.03.011

Recommended Citation

Qun-ying, XU and Yu-heng, ZHAI (2020) "Rapid detection of Alternaria monomethyl ether in wheat by high performance liquid chromatography," Food and Machinery: Vol. 36: Iss. 3, Article 11.
DOI: 10.13652/j.issn.1003-5788.2020.03.011
Available at: https://www.ifoodmm.cn/journal/vol36/iss3/11

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