Rapid detection of cadmium based on fluorescence molecular switch and aptamer

Authors

TU Dong-kun, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002 , China
LU Yue, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002 , China
ZHENG Xiao-liang, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002 , China
HUANG Min-li, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002 , China
WANG Yi-jing, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002 , China
ZENG Shao-xiao, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002 , China ；Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou, Fujian 350002 , China ；Fujian P
XU Hui, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002 , China ；Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou, Fujian 350002 , China ；Fujian P

Abstract

In order to study the fluorescent dye thiazole orange as a fluorescent molecular switch and a cadmium ion nucleic acid aptamer for the detection of cadmium ions. The effects were examined, from different pH values, thiazole orange-aptamer concentration ratio, binding time of thiazole orange to aptamer, and binding time of aptamer to cadmium ion on the fluorescence intensity of the system. The results showed that when the pH value was 7, the thiazole orange-aptamer concentration ratio was 7∶3, the binding time of thiazole orange and aptamer was 15 min, and the binding time of aptamer and cadmium ion was 20 min. Under these conditions, the fluorescence intensity and cadmium ion concentration showed a good linear relationship, with a linear range from 2.00 ng/mL to 80.00 ng/mL, and the detection limit was 0.23 ng/mL. This method does not require fluorescent labeling of the aptamer, and the operation is simple.

Publication Date

3-28-2020

First Page

63

Last Page

68

DOI

10.13652/j.issn.1003-5788.2020.03.012

Recommended Citation

Dong-kun, TU; Yue, LU; Xiao-liang, ZHENG; Min-li, HUANG; Yi-jing, WANG; Shao-xiao, ZENG; and Hui, XU (2020) "Rapid detection of cadmium based on fluorescence molecular switch and aptamer," Food and Machinery: Vol. 36: Iss. 3, Article 12.
DOI: 10.13652/j.issn.1003-5788.2020.03.012
Available at: https://www.ifoodmm.cn/journal/vol36/iss3/12

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