Optimization on ultrasonic-assisted extraction of polyphenols from Cyclobalanopsis glauca shells by response surface methodology and study on its antioxidant activity in vitro

Authors

RU Yue-rong, Forest Food Research Institute, Southwest Forestry University, Kunming, Yunnan 650224 , China ；College of Life Sciences, Southwest Forestry University, Kunming, Yunnan 650224 , China
ZHANG Zhi-yang, Forest Food Research Institute, Southwest Forestry University, Kunming, Yunnan 650224 , China ；College of Life Sciences, Southwest Forestry University, Kunming, Yunnan 650224 , China
YANG Jin-mei, Forest Food Research Institute, Southwest Forestry University, Kunming, Yunnan 650224 , China ；College of Life Sciences, Southwest Forestry University, Kunming, Yunnan 650224 , China
HE Bin, Forest Food Research Institute, Southwest Forestry University, Kunming, Yunnan 650224 , China ；College of Life Sciences, Southwest Forestry University, Kunming, Yunnan 650224 , China
KAN Huan, Forest Food Research Institute, Southwest Forestry University, Kunming, Yunnan 650224 , China ；College of Life Sciences, Southwest Forestry University, Kunming, Yunnan 650224 , China
LIU Yun, Forest Food Research Institute, Southwest Forestry University, Kunming, Yunnan 650224 , China ；College of Life Sciences, Southwest Forestry University, Kunming, Yunnan 650224 , China
WANG Zhen-xing, Forest Food Research Institute, Southwest Forestry University, Kunming, Yunnan 650224 , China ；College of Life Sciences, Southwest Forestry University, Kunming, Yunnan 650224 , China
ZHANG Xue-chun, Forest Food Research Institute, Southwest Forestry University, Kunming, Yunnan 650224 , China ；College of Life Sciences, Southwest Forestry University, Kunming, Yunnan 650224 , China

Abstract

The ultrasound-assisted extraction was applied to the extraction of polyphenols from the Cyclobalanopsis glauca fruit shells. The effects of the concentration of ethanol, solid-liquid ratio, ultrasonic time, and number of extraction cycles on the yield of phenolic yield were studied, and the extraction process was optimized by response surface methodology (RSM). Then the in vitro antioxidant activities of Cyclobalanopsis glauca shell fruit extract (CGSE), including ferric ion reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC), were evaluated. Results: The optimal extraction process were as followed: volume fraction of ethyl alcohol 50%, extracting time 25 min, solid-liquid ratio 1:40, extraction times 5. Under condition, the maximum extraction yield was 3.13%. The Cyclobalanopsis glauca Shells Extracts (CGSE) showed higher antioxidant activity with FRAP values of (984.44±123.15) mg FeSO₄/g·DW, and ORAC values of (200.15±23.24) mg Trolox/g·DW, which was higher than the commercial antioxidant 2,6-di-tert-butyl-4-methylphenol (BHT). The above results suggest that the Cyclobalanopsis glauca fruit shell might be a natural antioxidant resource.

Publication Date

3-28-2020

First Page

179

Last Page

184

DOI

10.13652/j.issn.1003-5788.2020.03.034

Recommended Citation

Yue-rong, RU; Zhi-yang, ZHANG; Jin-mei, YANG; Bin, HE; Huan, KAN; Yun, LIU; Zhen-xing, WANG; and Xue-chun, ZHANG (2020) "Optimization on ultrasonic-assisted extraction of polyphenols from Cyclobalanopsis glauca shells by response surface methodology and study on its antioxidant activity in vitro," Food and Machinery: Vol. 36: Iss. 3, Article 34.
DOI: 10.13652/j.issn.1003-5788.2020.03.034
Available at: https://www.ifoodmm.cn/journal/vol36/iss3/34

References

[1] 周伟,夏念和.我国壳斗科植物资源:尚待开发的宝库[J].林业资源管理,2011(2):93-96.
[2] 刘仁林,王娟,廖为明.10种壳斗科植物果实主要营养成分比较分析[J].江西农业大学学报,2009,31(5):901-905.
[3] 黄建琴,徐弈鼎,王烨军,等.安徽省橡实资源及其开发利用[J].安徽林业科技,2008(增刊1):27-28.
[4] 王亚凤,黄永林,刘金磊,等.壳斗科植物种子的多酚类含量及抗氧化能力[J].广西科学,2016,23(2):180-183.
[5] 周磊,许敏,杨崇仁,等.壳斗科植物的化学成分及生物活性研究进展[J].天然产物研究与开发,2012,24(2):260-273.
[6] 甘耀坤,陈旭健,韦敏,等.青冈栎果实抗癌活性的实验研究[J].食品科技,2010,35(3):227-229.
[7] 王敏,田珍燕,王蔚新.板栗壳多酚的提取及其抗氧化性能的研究[J].食品研究与开发,2018,39(22):66-72.
[8] 庞闹,王亚凤,何瑞杰,等.甜槠化学成分研究(Ⅰ)[J].中药材,2018,41(9):2 116-2 119.
[9] OGAWA K,HIROSE S,NAGAOKA S,et al.Interaction between tea polyphenols and bile acid inhibits micellar cholesterol solubility[J].Journal of Agricultural and Food Chemistry,2016,64(1):204-209.
[10] 王婧,郭阳,包怡红,等.仙人掌多酚超声辅助醇提工艺优化及抗氧化、降脂能力分析[J].食品与机械,2019,35(8):177-182.
[11] 阮冲,肖小华,李攻科.天然产物有效成分提取分离制备方法研究进展[J].化学试剂,2014,36(3):193-200.
[12] 刘远方,李萌萌,刘远晓,等.功率超声波及其在食品工业中的降解应用研究进展[J].食品与发酵工业,2018,44(10):283-289.
[13] PISOSCHI A M,POP A,CIMPEANU C,et al.Antioxidant capacity determination in plants and plant-derived products:A review[J].Oxidative Medicine and Cellular Longevity,2016,2 016:9130976.
[14] MANNINO G,PERRONE A,CAMPOBENEDETTO C,et al.Phytochemical profile and antioxidative properties of Plinia trunciflora fruits:A new source of nutraceuticals[J].Food Chemistry,2020,307:125515.
[15] BHOLAH K,RAMFUL-BABOOLALL D,NEERGHEEN-BHUJUN V S.Antioxidant activity of polyphenolic rich Moringa oleifera Lam.extracts in food systems[J].Journal of Food Biochemistry,2015,39(6):733-741.
[16] RODRIGUES C A,NICCIO A E,BOEING J S,et al.Rapid extraction method followed by a d-SPE clean-up step for determination of phenolic composition and antioxidant and antiproliferative activities from berry fruits[J].Food Chemistry,2020,309:125694.
[17] 秦永剑,张加研,杨兆勋.响应面优化油菜籽皮中原花青素的提取工艺[J].现代农业科技,2012(20):316-317.
[18] 马金魁,黄晓辰,张佳仪,等.响应面法优化辣木叶总黄酮提取工艺及其抑菌性研究[J].食品研究与开发,2019,40(15):57-65.
[19] HOFMANN T,NEBEHAJ E,ALBERT L.The high-performance liquid chromatography/multistage electrospray mass spectrometric investigation and extraction optimization of beech(Fagus sylvatica L.)bark polyphenols[J].Journal of Chromatography A,2015,1 393:96-105.
[20] 苏适,王双侠.响应曲面优化超声波辅助提取无花果叶总黄酮的工艺研究[J].食品研究与开发,2019,40(9):101-106.
[21] 张俞,刘婷婷,刘洋,等.蒙古栎种壳多酚的超声辅助提取及抗氧化能力[J].植物研究,2010,30(5):623-628.