Abstract
In this study, the biliary tolerance, acid resistance and cholesterol-lowering ability of 11 strains of Lactobacillus were studied, and the mechanism of removing cholesterol from Lactobacillus strains was explored. The cholesterol assimilation results determined by the difference in cholesterol content in the medium indicated that all Lactobacillus strains could assimilate cholesterol at a level of 12.03 to 32.25 μg/mL, and the cholesterol removal ability was related to the growth of the culture. The binding of cholesterol to lactobacilli cells was determined by growth, heat inactivation and dormant cells in phosphate buffer. The dry weight of cholesterol eliminated by heat and dormant cells was 0.79 to 3.82 mg/g; the dry weight of cholesterol cleared by growing cells was 4.53 to 16.03 mg/g. These results indicated that L. casei ASCC 292 and L. acidophilus 4962 strain exhibited good acid and biliary resistance, with the removal of most cholesterol from the medium. Lactobacillus strains could remove cholesterol by various mechanisms, and could be used as a dietary supplement to lower serum cholesterol in the body.
Publication Date
4-28-2020
First Page
77
Last Page
82
DOI
10.13652/j.issn.1003-5788.2020.04.014
Recommended Citation
Huan, WANG
(2020)
"Study on bile tolerance, acid resistance and cholesterol lowering ability of 11 strains of Lactobacillus,"
Food and Machinery: Vol. 36:
Iss.
4, Article 14.
DOI: 10.13652/j.issn.1003-5788.2020.04.014
Available at:
https://www.ifoodmm.cn/journal/vol36/iss4/14
References
[1] 黄燕燕,郭均,黎恒希,等.降胆固醇乳酸菌的体外筛选及其降胆固醇机理探讨[J].食品科学,2018(6):88-94.
[2] 李俊,卢阳,刘永翔,等.乳酸菌发酵对苦荞芽苗饮料品质和营养成分的影响[J].食品与机械,2018,34(12):201-205.
[3] 吕丹丹,何佳,宋文华,等.8种乳酸菌发酵对凤丹花瓣中酚类物质及其抗氧化活性的影响[J].食品与机械,2018,34(11):146-151.
[4] 韩墨,王燕,杨志鹏,等.内蒙古传统酸奶乳酸菌的筛选及体外益生效果评价[J].食品研究与开发,2018(1):152-156.
[5] 刘绒梅,田圆圆,耿琦,等.四川稻城传统干酪中乳酸菌的分离鉴定及功能研究[J].中国测试,2017(12):58-62.
[6] 苏爱美,魏绍春.胆道疾病患者胆汁菌群分布及耐药分析[J].中华实验和临床感染病杂志:电子版,2017,11(6):92-97.
[7] 范颖,陈思涵,党芳芳,等.一株降胆固醇乳酸菌的筛选及其在模拟消化环境活性的研究[J].中国乳品工业,2018,46(9):6-9.
[8] 杨晓艳.降胆固醇乳酸菌的筛选及其在低脂沙拉酱中的应用[D].邯郸:河北工程大学,2016:45-61.
[9] 陈珂可,朱建国,姜甜,等.具有降解胆固醇能力的益生菌菌株筛选[J].食品科技,2018,43(11):12-16.
[10] 李天艺,李华,焦文君,等.二甲双胍对老年2型糖尿病炎症和肠道菌群失衡的临床疗效研究[J].中华老年医学杂志,2018,37(8):879-882.
[11] 赵佳锐,范晓兵,杭晓敏,等.人体肠道益生菌体外降胆固醇活性研究[J].微生物学报,2005,45(6):920-924.
[12] 刘颖,张洁,高祀龙,等.酒精性肝病患者肠道中6种重要菌群的定量分析[J].营养学报,2018(1):36-41.
[13] 章文明,汪海峰,刘建新.乳酸杆菌益生作用机制的研究进展[J].动物营养学报,2012,24(3):389-396.
[14] 王越男,孙天松.代谢组学在乳酸菌发酵食品和功能食品中的应用[J].中国乳品工业,2017(5):27-31.
[15] 李瑛.浅析乳酸菌在食品工程中的应用[J].中国新技术新产品,2017(11):40-41.
[16] 王瑶,韦云路,李平兰.乳酸菌食品级质粒及应用的研究进展[J].食品科学,2017(13):275-282.
[17] 凌空,翟磊,姚粟,等.新疆传统发酵食品中乳酸菌的分离鉴定及其生长代谢特性[J].食品与发酵工业,2017(3):127-133.
[18] 刘芸,刘波,邓元源,等.食品稳定剂在植物蛋白乳酸菌饮料生产中的应用研究进展[J].福建农业学报,2017(9):127-133.
[19] 马国涵,马欢欢,吕欣然,等.大菱鲆肠道中广谱拮抗活性乳酸菌的筛选及其细菌素鉴定[J].食品科学,2019(6):159-165.
[20] 孙思睿,万峰,贺菁,等.乳酸菌细菌素的抑菌活性测定及效价表示方法[J].食品工业科技,2018,39(16):346-351.
[21] 徐颖,李洁,贺丹丹,等.两株乳酸菌对铅的吸附作用[J].食品与机械,2018,34(3):54-58.