Abstract
The purity characterization method for genistein certified reference material was established, and the its uncertainty generated during characterization was evaluated. In this study, we qualitatively analyzed genistein samples by Fourier transform infrared spectroscopy, mass spectrometry, and nuclear magnetic hydrogen spectrometry. Thereafter, two independent methods were used in the purity measurement of certified reference material (CRM) of main components. Moreover, the uniformity, stability and the uncertainty introduced in the whole setting were evaluated. Finally, the purity of the genistein CRM was confirmed to be 99.6%, and the expanded uncertainty was 0.4% (k=2).
Publication Date
4-28-2020
First Page
95
Last Page
101
DOI
10.13652/j.issn.1003-5788.2020.04.018
Recommended Citation
Quan-fa, LI; Han-song, YU; Min, WANG; and Jian, ZHOU
(2020)
"Determination of purity certified reference material of genistein and the evaluation of its uncertainty,"
Food and Machinery: Vol. 36:
Iss.
4, Article 18.
DOI: 10.13652/j.issn.1003-5788.2020.04.018
Available at:
https://www.ifoodmm.cn/journal/vol36/iss4/18
References
[1] 董李平.大豆异黄酮代谢机理的研究[D].重庆:西南农业大学,2003:6-9.
[2] 唐思颉,涂传海,胡文秀,等.红茶菌发酵黄浆水的体外抗氧化活性[J].食品科学,2019,40(17):1-6.
[3] 巫波,郭辉,王宗岭,等.大豆异黄酮的抗肿瘤作用研究进展[J].四川解剖学杂志,2019,27(3):184-187.
[4] 田笑,金梅花,刘莉园,等.大豆异黄酮诱导小鼠肝癌移植瘤细胞凋亡的机制[J].食品科学,2018,39(17):122-126.
[5] HUA P,TSAI W J,KUO S M.Estrogen response element-independent regulation of gene expression by genistein in intestinal cells[J].Biochimica et Biophysica Acta(BBA)-Gene Structure and Expression,2003,1 627(2):63-70.
[6] 李恋曲,王晓钰,贾志荣,等.异黄酮类植物雌激素防治过敏性疾病研究进展[J].中国药理学通报,2018,34(4):453-456.
[7] 张翠芬.大豆异黄酮对心血管疾病的研究综述[J].中国食品添加剂,2018(9):210-213.
[8] 王莹,田蜜,贺红,等.大豆异黄酮对围绝经期和绝经后妇女安全性的Meta分析[J].毒理学杂志,2018,32(4):277-286.
[9] VAHID D M,ESLAMI B,ALSADAT S M,et al.Effect of soy isoflavone on hot flushes,endometrial thickness,and breast clinical as well as sonographic features[J].Iran J Public Health,2018,47(3):382-389.
[10] WADA K,TSUJI M,TAMURA T,et al.Soy isoflavone intake and bladder cancer risk in Japan:From the takayama study[J].Cancer Epidemiol Biomarkers Prev,2018,27(11):1 371-1 375.
[11] 王路,卢卫红,程翠琳,等.同时检测人尿中六种大豆异黄酮代谢产物的HPLC法[J].营养学报,2009,31(2):181-184.
[12] PORTER P M,BANWART W L,HASSETT J J.HPLC isolation and GC-MS identification of genistein,daidzein,and coumestrol from unhydrolyzed soybean root extrac-ts[J].Environmental and Experimental Botany,1985,25(3):229-232.
[13] ZHAO Xin,MA Fei,LI Pei-wu,et al.Simultaneous determination of isoflavones and resveratrols for adulteration detection of soybean and peanut oils by mixed-mode SPE LC-MS/MS[J].Food Chem,2015,176:465-471.
[14] XU Hai-yan,ZHENG Hua-chuan,ZHANG Hui-wen,et al.Comparison of antioxidant constituents of agriophyllum squarrosum seed with conventional crop seeds[J].J Food Sci,2018,83(7):1 823-1 831.
[15] GOMEZ J D,VITAL C E,OLIVEIRA M G A,et al.Broad range flavonoid profiling by LC/MS of soybean genotypes contrasting for resistance to Anticarsia gemmatalis(Lepidoptera:Noctuidae)[J].PLoS One,2018,13(10):e0205010.
[16] 孙艳梅.β-葡萄糖苷酶水解大豆异黄酮糖苷的研究[D].哈尔滨:东北农业大学,2003:29-32.
[17] FERRER I,BARBER L B,THURMAN E M.Gas chromatographic-mass spectrometric fragmentation study of phytoestrogens as their trimethylsilyl derivatives:Identification in soy milk and wastewater samples[J].J Chromatogr A,2009,1 216(32):6 024-6 032.
[18] ROSTAGNO M A,VILLARES A,GUILLAMON E,et al.Sample preparation for the analysis of isoflavones from soybeans and soy foods[J].J Chromatogr A,2009,1 216(1):2-29.
[19] GONG Ning-bo,WANG Xiao-ying,ZHANG Bao-xi,et al.Characterization and uncertainty evaluation of a new certified reference material of baicalein[J].Journal of Pharmaceutical and Biomedical Analysis,2014,89:106-110.
[20] YANG De-zhi,YANG Shi-ying,ZHANG Bao-xi,et al.Certification of reference materials for analysis of isoflavones genistin and genistein in soy products[J].Analytical Methods,2016,8(1):89-96.
[21] 周剑,齐鑫,杨梦瑞,等.甲醇中克伦特罗、莱克多巴胺和沙丁胺醇3种溶液标准物质的定值及不确定度评估[J].化学试剂,2019,41(5):462-468.
[22] RACKHAM D M.Recent applications of quantitative nuclear magnetic resonance spectroscopy in pharmaceutical research[J].Talanta,1976,23(4):269-274.
[23] 李珍柱.三种类黄酮纯度标准物质的研制[D].重庆:西南大学,2018:20-24.
[24] 文瑞芝,李水芳,曾栋.染料木素和大豆素的结构分析[J].大豆科学,2008(1):128-133.
[25] 韩永志,余逵,刘清贤.JJF 1006—1994 一级标准物质技术规范[S].北京:国家标准物质研究中心,1994:14.
[26] 阚盈,李红梅,孟凡敏,等.JJF 1343—2012标准物质定值的通用原则及统计学原理[M].北京:中国计量科学研究院,2012.
[27] TANGPAISARNKUL N,TUCHINDA P,WILAIRAT P,et al.Development of pure certified reference material of stevioside[J].Food Chem,2018,255:75-80.