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Abstract

Strains with biodegradability of biogenic amines were screened from lactic acid bacteria strains from different sources preserved in the laboratory, and their growth ability at different salt concentrations and pH values were determined. The results showed that three strains with the capability of degrading biogenic amines were screened out. Based on 16S rDNA sequence analysis, strain HM22 was identified as Lactobacillus fermentum, strain HM24 as Lactobacillus plantarum, and strain YF10042 as Enterococcus faecalis. Among them, Lactobacillus plantarum HM24 has the best degradation ability. The degradation rates of tryptamine, phenethylamine, putrescine, cadaverine, histamine, tyramine, spermidine and spermine are (25.90±2.30)%, (35.75±2.71)%, (27.61±3.94)%, (25.91±3.76)%, (22.54±2.64)%, (34.55±1.90)%, (39.25±1.86)%, (55.66±7.08)%, respectively. When the salt concentration is 4%~10%, the tolerance of 3 strains decreased with the increase of salt concentration; When pH is 3.5~9.5, all three strains can grow.

Publication Date

5-28-2020

First Page

64

Last Page

69

DOI

10.13652/j.issn.1003-5788.2020.05.012

References

[1] OHNUMA S,HIGA M,HAMANAKA S,et al.An outbreak of allergy-like food poisoning[J].Internal medicine,2001,40(8):833-835.
[2] 王光强,俞剑燊,胡健,等.食品中生物胺的研究进展[J].食品科学,2016,37(1):269-278.
[3] ZOGUL Y,ZOGUL F.Biogenic amines formation,toxicity,regulations in food[M].England:Royal Society of Chemistry publishing,2019:1-17.
[4] 龚景龙.发酵食品中的生物胺问题及控制对策[J].食品界,2019(8):57.
[5] 肖洪,丁晓雯,梁菡峪,等.发酵食品中的生物胺及其控制研究进展[J].食品工业科技,2012,33(20):346-350.
[6] 景智波,田建军,杨明阳,等.食品中与生物胺形成相关的微生物菌群及其控制技术研究进展[J].食品科学,2018,39(15):262-268.
[7] 邓红梅,卢士玲,李开雄.传统中式香肠中产生物胺氧化酶菌的分离鉴定[J].食品与发酵工业,2012,38(2):57-60.
[8] 吴燕燕,钱茜茜,陈玉峰,等.咸鱼中生物胺降解菌的筛选与降解特性研究[J].食品工业科技,2016,37(18):173-179.
[9] 郝宇,孙波,张宇,等.高效液相色谱法测定东北农家酱中的5种生物胺[J].食品科学,2019,40(16):343-349.
[10] 曾雪晴,李洪军,袁琳娜,等.郫县豆瓣酱中生物胺含量和种类分析[J].食品与发酵工业,2019,45(19):258-265.
[11] 王丽芳,王修俊,郑君花,等.发酵辣椒中一株乳酸菌的分离鉴定及其生长特性的研究[J].中国调味品,2014(6):67-72.
[12] 徐鑫,王茜茜,王晓蕊,等.传统农家大酱中耐盐性乳酸菌的分离与鉴定[J].食品与发酵工业,2014,40(11):33-40.
[13] 方祥,胡文锋,张辉华,等.乳酸菌的分离、鉴定及其生长特性[J].中国微生态学杂志,2000,12(5):262-264.
[14] 孙霞.四川香肠中生物胺降解菌的筛选鉴定及其初步应用[D].成都:四川农业大学,2016:23.
[15] 张楠,杨勇,李彬彬,等.四川自然发酵香肠中组胺降解菌的筛选鉴定及初步应用[J].食品与发酵工业,2018,44(1):72-79.
[16] 刘玉晗,卢士玲,陆静,等.乳酸菌中生物胺氧化酶菌株的筛选[J].现代食品科技,2016,32(4):106-113.
[17] 张菁.黄豆酱发酵过程中生物胺形成的影响因素研究[D].天津:天津科技大学,2014:31.
[18] 毛顺,黄笠原,张惠超,等.不同条件对两株乳酸菌降解生物胺的影响[J].食品工业科技,2018,39(16):132-137.
[19] 杨利昆,付湘晋,胡叶碧,等.鱼露中生物胺降解菌的筛选及其特性[J].食品科学,2012,33(11):158-162.
[20] FADDA S,VIGNOLO G,OLIVER G.Tyramine degradation and tyramine/histamine production by lactic acid bacteria and Kocuria strains[J].Biotechnology Letters,2001,23(24):2 015-2 019.
[21] 张沁芳.植物乳杆菌组胺分解酶的分离纯化及性质研究[D].烟台:烟台大学,2018:4.
[22] 谢翀.应用植物乳杆菌降低发酵香肠中生物胺含量的研究[D].南京:南京农业大学,2015:45.
[23] 徐建芬,毛杰琪,魏晓璐,等.黄酒中不产生物胺乳酸菌的筛选及应用[J].食品与机械,2017,33(9):20-25.
[24] ALVAREZ M A,MORENO-ARRIBAS M V.The problem of biogenic amines in fermented foods and the use of potential biogenic amine-degrading microorganisms as a solution[J].Food Science & Technology,2014,39(2):146-155.

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