Abstract
In this study, the water-extracted ethanol precipitation method was used to extract Polysaccharides in Pyropolyporus Formentarius (PFPS), and the capacities of scavenging DPPH and ABTS were analyzed. Different concentrations of PFPS were used to detect the resistant abilities against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis, and Micrococcus tetragenus, and the relative minimum inhibitory concentration (MIC) were also determined. The results showed that PFPS could eliminate DPPH and ABTS. When the concentration of PFPS was 1.0 mg/mL, the ability of scavenging DPPH and ABTS exceeded that of the BHT control, while was weaker than the VC reference substance. The antioxidant activity of PFPS with different concentrations was significantly different. PFPS had inhibitory effects on the four tested bacteria and there were significant differences, and the strongest inhibitory activity was resistant against E. coli. The minimum inhibitory concentrations of E. coli, S. aureus, B. subtilis, and M. tetragenus were 1.25, 1.25, 2.50, 5.00 mg/mL, respectively. In summary, PFPS had good antioxidant and antibacterial activities, with gradually increasing associated with the polysaccharide concentration.
Publication Date
7-28-2020
First Page
171
Last Page
174
DOI
10.13652/j.issn.1003-5788.2020.07.035
Recommended Citation
Hai-yan, XU; Xiu-juan, PENG; Shan, WANG; Yan-hong, LIU; and Feng, LIU
(2020)
"Study on antioxidant and antibacterial activities ofpolysaccharides in Phropolyporus fomentarius,"
Food and Machinery: Vol. 36:
Iss.
7, Article 35.
DOI: 10.13652/j.issn.1003-5788.2020.07.035
Available at:
https://www.ifoodmm.cn/journal/vol36/iss7/35
References
[1] 张艳华.桦菌芝抗肿瘤研究及其机制探讨[D].西安:陕西师范大学,2015:30.
[2] 黑育荣,彭修娟,杨新杰.桦菌芝化学成分及药理活性研究进展[J].农产品加工,2019,17(16):71-72.
[3] 王世龙.桦菌芝多糖对H22小鼠瘤组织中Bax、Bcl-2的影响[D].哈尔滨:黑龙江中医药大学,2013:22-23.
[4] 齐彦,王瑶,郭丽新,等.桦菌芝多糖的抗突变作用研究[J].中医药信息,2011,28(1):21-23.
[5] 张文晋,王升,黄璐琦,等.中药多糖质量评控方法探析[J/OL].中国中药杂志.(2020-03-03)[2020-03-06].https://doi.org/10.19540/j.cnki.cjcmm.20200229.201.
[6] 周欣,付志飞,谢燕,等.中药多糖对肠道菌群作用的研究进展[J].中成药,2019,41(3):623-627.
[7] 张彦洁,章丽雅,吴菁,等.糖生物学与免疫调控的病生理意义及进展[J].生命科学,2013,25(1):20-27.
[8] 孟祥云,汪永锋,杨丽霞,等.中药多糖抗氧化作用及其机制研究进展[J].中华中医药杂志,2018,33(8):3 504-3 509.
[9] 周云冬.植物提取物抑菌活性及抑菌机理[D].无锡:江南大学,2019:11-12.
[10] 陈胜发,彭修娟,顾国强,等.响应面法优化桦菌芝多糖的超声辅助提取工艺[J].化学与生物工程,2018,35(10):31-35.
[11] 刘婷.胡桃醌的抗氧化、抑菌活性及抑菌机理研究[D].临汾:山西师范大学,2018:8-9.
[12] 刘胜帅,薛楠楠,段银祥,等.绿花白千层叶油的GC-MS分析及其体外抑菌、抗炎活性研究[J].中国药学杂志,2019,54(16):1 292-1 298.
[13] 尹显楼,詹济华,谭洋,等.铁苋菜不同极性萃取物的抗氧化及抑菌活性研究[J].食品与机械,2019,35(6):172-176.
[14] 许海燕,李亚玲,彭修娟,等.秦岭龙胆挥发油成分分析及抗氧化活性研究[J].中药新药与临床药理,2019,30(1):106-109.
[15] 吴永祥,程满怀,江海涛,等.白及萃取物的抑菌活性及其二氯甲烷萃取物化学成分分析[J].食品与机械,2017,33(12):76-79.
[16] 延永,李玉萌,张亦琳,等.红薯叶总黄酮的提取工艺优化及其抑菌、抗氧化活性研究[J].广西林业科学,2018,47(3):311-315.
[17] YANG Hui,HUA Jun-li,WANG Chuang.Anti-oxidation and anti-aging activity of polysaccharide from Malus micromalus Makino fruit wine[J].Int J Biol Macromol,2019,121:1 203-1 212.