Optimization of extraction process and quality analysis of Pseudosciaena crocea liver oil by response surface methodology

Authors

DOU Xin, South China Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences, Key Laboratoryof Aquatic Products Processing, Ministry of Agriculture and Rural Areas, Guangzhou, Guangdong 510300 ,China ；College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306 , China
WU Yan-yan, South China Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences, Key Laboratoryof Aquatic Products Processing, Ministry of Agriculture and Rural Areas, Guangzhou, Guangdong 510300 ,China
YANG Xian-qing, South China Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences, Key Laboratoryof Aquatic Products Processing, Ministry of Agriculture and Rural Areas, Guangzhou, Guangdong 510300 ,China
HU Xiao, South China Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences, Key Laboratoryof Aquatic Products Processing, Ministry of Agriculture and Rural Areas, Guangzhou, Guangdong 510300 ,China
WANG Yue-qi, South China Sea Fisheries Research Institute of Chinese Academy of Fishery Sciences, Key Laboratoryof Aquatic Products Processing, Ministry of Agriculture and Rural Areas, Guangzhou, Guangdong 510300 ,China

Abstract

The large yellow croaker liver was used as the raw material, and the optimal protease was used to extract and extract the large yellow croaker liver oil. Taking the large yellow croaker liver oil extraction rate as an evaluation index, the best enzymatic extraction process was determined through single-factor experiments and response surface experiments. The extraction rate, quality, and fatty acid composition of the large yellow croaker liver oil were compared with those obtained by light alkali method. The results showed that the best enzymatic extraction conditions were as followed: for large yellow croaker liver was using neutral protease, the ratio of material liquid ratio was 1∶2, the amount of enzyme added was 2.5%, the value of pH was 7.3, the hydrolysis time was 4 h, and the hydrolysis temperature was 50.3 ℃. Under the conditions, the extraction rate of large yellow croaker liver oil was 78.39%, and the quality was better than that of light alkali method. The oil was clear, the acid value was (5.83±0.15) mg/g, the iodine value was (142.65±0.22) mg / 100 g. There were 13 kinds of fatty acids, 5 more than that of light alkali method. There are 8 kinds of unsaturated fatty acids, including 19.71 g/100 g saturated fatty acids, 62.63 g/100 g monounsaturated fatty acids and 17.62 g/100 g polyunsaturated fatty acids. The extraction rate, quality and fatty acid composition of liver oil from large yellow croaker by enzymatic method were better than those by alkali method.

Publication Date

7-28-2020

First Page

187

Last Page

193

DOI

10.13652/j.issn.1003-5788.2020.07.038

Recommended Citation

Xin, DOU; Yan-yan, WU; Xian-qing, YANG; Xiao, HU; and Yue-qi, WANG (2020) "Optimization of extraction process and quality analysis of Pseudosciaena crocea liver oil by response surface methodology," Food and Machinery: Vol. 36: Iss. 7, Article 38.
DOI: 10.13652/j.issn.1003-5788.2020.07.038
Available at: https://www.ifoodmm.cn/journal/vol36/iss7/38

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