Abstract
Based on the traditional colloidal strip, the pre-incubation reaction model was adopted, which was performed in the homogeneous aqueous solution between the colloid gold labeled antibody and analyte. This pre-incubation reaction was used to improve the efficiency of the immunoreaction. The pre-incubation solution was added to the colloidal gold strip for detection. Taking advantage of the pre-incubation model, the pre-incubated products were added to the colloidal gold strip for detection and the sensitivity for tetracycline was improved by 5 folds, as low as 1 μg/L. Changing the reaction model of the traditional colloidal gold strip from the direction adding sample to pre-incubation and adding sample could obviously improve the sensitivity of the traditional colloidal gold strip method.
Publication Date
2-18-2023
First Page
57
Last Page
61
DOI
10.13652/j.issn.1003-5788.2020.08.010
Recommended Citation
Ju-yi, CHEN; Yu-han, WU; Li, YAO; Zhou, XU; Qian, WU; and Wei, CHEN
(2023)
"Study of tetracycline detection in milk by pre-incubation mediated signal enhanced colloidal gold method,"
Food and Machinery: Vol. 36:
Iss.
8, Article 10.
DOI: 10.13652/j.issn.1003-5788.2020.08.010
Available at:
https://www.ifoodmm.cn/journal/vol36/iss8/10
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