Abstract
A simple and rapid method for the simultaneous determination of 7 kinds of hormones in grass carp was developed by the QuEChERS method coupled with ultra performance liquid chromatogragphy quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q/Orbitrap MS). The results indicated that the linearity of all the 7 hormones ranged from 1.0 ng/mL to 100.0 ng/mL with the correlation coefficients greater than 0.998. The LOD was in the range of 0.04~0.60 μg/kg and the LOQ was in the range of 0.2~1.8 μg/kg. The recoveries were in the range of 71.4% to 106.9%, with the RSD (n=6) in the range of 2.4%~7.8%. 7 hormones showed matrix inhibition effects, and the matrix effects were compensated by blank matrix matching standard curve to obtain accurate results. The method is simple, rapid, accurate, and suitable for the rapid detection of glucocorticoids in grass carp.
Publication Date
2-18-2023
First Page
72
Last Page
76, 116
DOI
10.13652/j.issn.1003-5788.2020.08.013
Recommended Citation
Yan-hua, ZHOU; Tao, LI; and Peng-fei, ZHANG
(2023)
"Rapid determination of 7 hormones in grass carp using QuEChERS coupled with UPLC-Q/ Orbitrap MS,"
Food and Machinery: Vol. 36:
Iss.
8, Article 13.
DOI: 10.13652/j.issn.1003-5788.2020.08.013
Available at:
https://www.ifoodmm.cn/journal/vol36/iss8/13
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