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Abstract

This review covered the advances in the screening of microbial pectinase, breeding technology, some factors affecting pectinase production, enzymatic properties, immobilization, and the development prospects of microbial pectinase were outlined.

Publication Date

2-18-2023

First Page

201

Last Page

209

DOI

10.13652/j.issn.1003-5788.2020.08.035

References

[1] SHARMA N,RATHORE M,SHARMA M.Microbial pectinase:Sources,characterization and applications[J].Reviews in Environmental Science and Bio/Technology,2013,12(1):45-60.
[2] AMIN F,BHATTI H N,BILAL M.Recent advances in the production strategies of microbial pectinases:A review[J].International Journal of Biological Macromolecules,2019,122:1 017-1 026.
[3] GUAN Yi,YIN Di,DU Xi,et al.Functional metabolomics approach reveals the reduced biosynthesis of fatty acids and TCA cycle is required for pectinase activity in Bacillus licheniformis[J].Journal of Industrial Microbiology and Biotechnology,2018,45(11):951-960.
[4] AKINYEMI B,BURAIMOH O,OGUNRINDE O,et al.Pectinase production by Bacillus and Paenibacillus sp.isolated from decomposing wood residues in the lagos lagoon[J].Journal of Tropical Life Science,2017,7(3):204-207.
[5] ZHOU Man,WU Jing-li,WANG Tao,et al.The purification and characterization of a novel alkali-stable pectate lyase produced by Bacillus subtilis PB1[J].World Journal of Microbiology and Biotechnology,2017,33(10):1-13.
[6] 谭秀华.高温耐碱性果胶酶菌株的筛选及其产酶的分离纯化研究[J].生物学杂志,2016,33(4):25-28.
[7] BENNAMOUN L,HILIGSMANN S,DAKHMOUCHE S,et al.Production and properties of a thermostable,pH-stable exo-polygalacturonase using Aureobasidium pullulans isolated from Saharan soil of Algeria grown on tomato pomace[J].Foods,2016,5(4):1-20.
[8] HANNAN A,BAJWA R,LATIF Z.Screening of Aspergillus niger strains for pectinases production potential[J].Pakistan Journal of Phytopathology,2009,21(1):77-82.
[9] GOPHANEA S R,KHOBRAGADEA C N,JAYEBHAYEA S G.Extracellular pectinase activity from Bacillus cereus GC subgroup A:Isolation,production,optimization and partial characterisation[J].Journal of Microbiology,Biotechnology and Food Sciences,2016,6(2):767-772.
[10] 贾兰兰,程超,王金鑫,等.高产果胶酶酵母菌株筛选及产酶条件研究[J].甘肃农业大学学报,2016,51(5):154-160.
[11] 宁振兴,陈皓睿,刘鸿,等.一株能高效降解梗丝果胶和纤维素的曲霉的筛选和鉴定[J].基因组学与应用生物学,2016,35(7):2 077-2 082.
[12] 徐鹏,王大红,陈亚欣,等.一株大麻脱胶菌株的分离鉴定及其产果胶酶发酵培养基的优化[J].中国麻业科学,2019,41(3):122-129.
[13] GUAN Yi,WANG Dong-fang,LV Chao,et al.Archives of microbiology:Screening of pectinase-producing bacteria from citrus peel and characterization of a recombinant pectate lyase with applied potential[J/OL].Archives of Microbiology.[2020-01-13].http://link.springer.com/10.1007/s00203-020-01807-0.
[14] HAILE M,KANG W H.Isolation,identification,and characterization of pectinolytic yeasts for starter culture in coffee fermentation[J].Microorganisms,2019,7(10):1-16.
[15] 杨斯超.产低温果胶酶酵母菌的筛选、产酶条件优化及酶学性质表征[D].吉林:吉林大学,2019:15.
[16] 卢晓华,杨苗,王常高,等.产果胶酶菌株的筛选鉴定及产酶条件优化[J].食品工业科技,2016,37(2):189-194.
[17] 周熠,黄河,冯波,等.一株碱性果胶酶产生菌的筛选、鉴定及发酵条件优化[J].湖北农业科学,2016,55(4):992-996.
[18] POVEDA G,GIL-DURAN C,VACA I,et al.Cold-active pectinolytic activity produced by filamentous fungi associated with Antarctic marine sponges[J].Biological Research,2018,51(1):28.
[19] DASKAYA-DIKMEN C,KARBANCIOGLU-GULER F,OZCELIK B.Cold active pectinase,amylase and protease production by yeast isolates obtained from environmental samples[J].Extremophiles,2018,22(4):599-606.
[20] 尹乐斌,雷志明,孔彦卓,等.一株高产果胶酶的真菌分离鉴定及酶学特性[J].中国酿造,2017,36(2):93-97.
[21] 李治玲,李勇,李世忠,等.柑橘皮渣降解菌的筛选及产酶条件优化[J].西南大学学报(自然科学版),2016,38(1):159-164.
[22] ZHANG Ge,LI Shu-gui,XU Ying-bo,et al.Production of alkaline pectinase:A case study investigating the use of tobacco stalk with the newly isolated strain Bacillus tequilensis CAS-MEI-2-33[J].BMC Biotechnology,2019,19(1):45.
[23] HUANG Dan-mei,SONG Yu-yang,LIU Yan-lin,et al.A new strain of Aspergillus tubingensis for high-activity pectinase production[J].Brazilian Journal of Microbiology,2019,50(1):53-65.
[24] KABIR M S,TASMIM T.Isolation of pectinase producing bacteria from the rhizosphere of Andrographis paniculata Nees and 16s rRNA gene sequence comparison of some potential strains[J].Advances in Microbiology,2019,9(1):1-13.
[25] KAUR S,KAUR H P,PRASAD B,et al.Production and optimization of pectinase by Bacillus sp.isolated from vegetable waste soil[J].Indo American Journal of Pharmaceutical Research,2016,6(1):485-490.
[26] ABD EL-RAHIM W M,MOAWAD H,HASHEM M M,et al.Highly efficient fungal pectinase and laccase producers among isolates from flax retting liquor[J].Biocatalysis and Agricultural Biotechnology,2020,25:101570.
[27] ROY K,DEY S,UDDIN M K,et al.Extracellular pectinase from a novel bacterium Chryseobacterium indologenes strain sd and its application in fruit juice clarification[J].Enzyme Research,2018,2 018:3859752.
[28] MOHANDAS A,RAVEENDRAN S,PARAMESWARAN B,et al.Production of pectinase from Bacillus sonorensis MPTD1[J].Food Technology and Biotechnology,2018,56(1):110-116.
[29] BIBI N,ALI S,TABASSUM R.Isolation and identification of novel indigenous bacterial strain as a low cost pectinase source[J].Brazilian Archives of Biology and Technology,2018,61:1-7.
[30] BHARADWAJ P S,UDUPA P M.Isolation,purification and characterization of pectinase enzyme from Streptomyces thermocarboxydus[J].Journal of Clinical Microbiology and Biochemical Technology,2019,5(1):1-6.
[31] RAHMAN M S,CHOI Y S,KIM Y K,et al.Production of novel polygalacturonase from Bacillus paralicheniformis CBS32 and application to depolymerization of ramie fiber[J].Polymers:Basel,2019,11(9):1-11.
[32] 张玉杰,李越博,侯丽娟,等.高产红枣果胶酶菌株的筛选及鉴定[J].食品科技,2015,40(10):17-21.
[33] MUZZAMAL H,LATIF Z.Improvement of Bacillus strains by mutation for overproduction of exopolygalacturonases[J].Indian Journal of Experimental Biology,2016,54(8):509-517.
[34] 杜国军,王殿友,刘晓兰,等.高产果胶酶黑曲霉的复合诱变选育[J].食品工业,2014,35(12):1-4.
[35] 黄丹梅,宋育阳,刘延琳,等.产果胶酶黑曲霉的UV诱变及基于葡萄皮渣的固态发酵[J].食品工业科技,2017,38(17):125-130.
[36] 何海燕,覃拥灵,陆世则,等.产果胶酶棘孢曲霉的筛选鉴定及微波诱变育种[J].中国饲料,2015(2):20-22.
[37] OTTENHEIM C,NAWRATH M,WU Jin-chuan.Microbial mutagenesis by atmospheric and room-temperature plasma(ARTP):The latest development[J].Bioresources and Bioprocessing,2018,5:1-12.
[38] ZHANG Xue,ZHANG Xiao-fei,LI He-ping,et al.Atmospheric and room temperature plasma(ARTP)as a new powerful mutagenesis tool[J].Applied Microbiology and Biotechnology,2014,98(12):5 387-5 396.
[39] YUAN Ye,ZHANG Xin-yu,ZHAO Yan,et al.A novel PL9 pectate lyase from Paenibacillus polymyxa KF-1:Cloning,expression,and its application in pectin degradation[J].International Journal of Molecular Sciences,2019,20(12):1-17.
[40] TANG Yu-meng,WU Pan,JIANG Si-jing,et al.A new cold-active and alkaline pectate lyase from Antarctic bacterium with high catalytic efficiency[J].Applied Microbiology and Biotechnology,2019,103(13):5 231-5 241.
[41] LIU Ming-qi,HUO Wen-kang,DAI Xian-jun,et al.Preparation of low-molecular-weight citrus pectin by recombinant Bacillus subtilis pectate lyase and promotion of growth of Bifidobacterium longum[J].Catalysis Communications,2018,107:39-42.
[42] ZHOU Cheng,XUE Yan-fen,MA Yan-he.Cloning,evaluation,and high-level expression of a thermo-alkaline pectate lyase from alkaliphilic Bacillus clausii with potential in ramie degumming[J].Applied Microbiology and Biotechnology,2017,101(9):3 663-3 676.
[43] KAMIJO J,SAKAI K,SUZUKI H,et al.Identification and characterization of a thermostable pectate lyase from Aspergillus luchuensis var.saitoi[J].Food Chemistry,2019,276:503-510.
[44] 莫健新,王豪强,黄广燕,等.微生物源果胶酶在猪PK15细胞中异源表达及其酶学性质分析[J].遗传,2019,41(8):736-745.
[45] WANG Xiao-wen,LU Zheng-hui,XU Ting,et al.Improving the specific activity and thermo-stability of alkaline pectate lyase from Bacillus subtilis 168 for bioscouring[J].Biochemical Engineering Journal,2018,129:74-83.
[46] ZHOU Cheng,YE Jin-tong,XUE Yan-fen,et al.Directed evolution and structural analysis of alkaline pectate lyase from the alkaliphilic Bacterium bacillus sp.strain N16-5 to improve its thermostability for efficient ramie degumming[J].Applied and Environmental Microbiology,2015,81(17):5 714-5 723.
[47] YU Ping,WANG Xin-xin,REN Qian,et al.Genome shuffling for improving the activity of alkaline pectinase in Bacillus subtilis FS105 and its molecular mechanism[J].World Journal of Microbiology and Biotechnology,2019,35(11):1-10.
[48] LI Pei-jun,XIA Jin-lan,SHAN Yang,et al.Optimizing production of pectinase from orange peel by Penicillium oxalicum PJ02 using response surface methodology[J].Waste and Biomass Valorization,2014,6(1):13-22.
[49] GUO Fen-fen,LI Xue-zhi,ZHAO Jian,et al.Optimizing culture conditions by statistical approach to enhance production of pectinase from Bacillus sp.Y1[J].Biomed Research International,2019,2 019:1-10.
[50] YU Ping,ZHANG Yi-shu,GU Dong-lu.Production optimization of a heat-tolerant alkaline pectinase from Bacillus subtilis ZGL14 and its purification and characterization[J].Bioengineered,2017,8(5):613-623.
[51] JAHAN N,SHAHID F,AMAN A,et al.Utilization of agro waste pectin for the production of industrially important polygalacturonase[J].Heliyon,2017,3(6):1-13.
[52] ABDULLAH R,JAFER A,NISAR K,et al.Process optimization for pectinase production by locally isolated fungal strain using submerged fermentation[J].Bioscience Journal,2018,3(4):1 025-1 032.
[53] AMIN F,BHATTI H N,BILAL M,et al.Multiple parameter optimizations for enhanced biosynthesis of exo-polygalacturonase enzyme and its application in fruit juice clarification[J].International Journal of Food Engineering,2017,13(2):1-14.
[54] CHEN Yu-quan,LIU Yu-ting,QIU Jie,et al.Co-expression of protease and pectinase in Bacillus subtilis using the herbal saponin extract as substrate[J].International Microbiology,2018,21(4):223-229.
[55] AHMED A,KHAN M N,AHMAD A,et al.Optimization of pectinase production from Geotrichum candidum AA15 using response surface methodology[J].Pakistan Journal of Botany,2019,51(2):743-750.
[56] WONG Li-yin,SAAD W Z,MOHAMAD R,et al.Optimization of cultural conditions for polygalacturonase production by a newly isolated Aspergillus fumigatus R6 capable of retting kenaf[J].Industrial Crops and Products,2017,97:175-183.
[57] MAHESH M,ARIVIZHIVENDHAN K V,MAHARAJA P,et al.Production,purification and immobilization of pectinase from Aspergillus ibericus onto functionalized nanoporous activated carbon(FNAC)and its application on treatment of pectin containing wastewater[J].Journal of Molecular Catalysis B:Enzymatic,2016,133:43-54.
[58] DEMIR H,TARI C.Effect of physicochemical parameters on the polygalacturonase of an Aspergillus sojae mutant using wheat bran,an agro-industrial waste,via solid-state fermentation[J].Journal of the Science of Food and Agriculture,2016,96(10):3 575-3 582.
[59] BIBI N,ALI S,TABASSUM R.Statistical optimization of pectinase biosynthesis from orange peel by Bacillus licheniformis using submerged fermentation[J].Waste and Biomass Valorization,2016,7(3):467-481.
[60] ORTIZ G E,PONCE-MORA M C,NOSEDA D G,et al.Pectinase production by Aspergillus giganteus in solid-state fermentation:Optimization,scale-up,biochemical characterization and its application in olive-oil extraction[J].Journal of Industrial Microbiology and Biotechnology,2017,44(2):197-211.
[61] KAUR S J,GUPTA V K.Production of pectinolytic enzymes pectinase and pectin lyase by Bacillus subtilis SAV-21 in solid state fermentation[J].Annals of Microbiology,2017,67(4):333-342.
[62] ZHANG Jian,ZHAO Li,GAO Bei,et al.Protopectinase production by Paenibacillus polymyxa Z6 and its application in pectin extraction from apple pomace[J].Journal of Food Processing and Preservation,2018,42(1):1-8.
[63] UZUNER S,CEKMECELIOGLU D.Enhanced pectinase production by optimizing fermentation conditions of Bacillus subtilis growing on hazelnut shell hydrolyzate[J].Journal of Molecular Catalysis B:Enzymatic,2015,113:62-67.
[64] YU Ping,XU Chao-chao.Production optimization,purification and characterization of a heat-tolerant acidic pectinase from Bacillus sp.ZJ1407[J].International Journal of Biological Macromolecules,2018,108:972-980.
[65] HANDA S,SHARMA N,PATHANIA S.Multiple parameter optimization for maximization of pectinase production by Rhizopus sp.C4 under solid state fermentation[J].Fermentation,2016,2(4):1-9.
[66] AHMED A,EJAZ U,SOHAIL M.Pectinase production from immobilized and free cells of Geotrichum candidum AA15 in galacturonic acid and sugars containing medium[J].Journal of King Saud University-Science,2020,32(1):952-954.
[67] EJAZ U,AHMED A,SOHAIL M.Statistical optimization of immobilization of yeast cells on corncob for pectinase production[J].Biocatalysis and Agricultural Biotechnology,2018,14:450-456.
[68] KUMAR P G,SUNEETHA V.Comparative initial study of polygalacturonase production by Bacillus VIT-Sun2 and Actinomycetes VIT-SP5[J].Research Journal of Biotechnology,2017,12(9):49-55.
[69] BARMAN S,SIT N,BADWAIK L S,et al.Pectinase production by Aspergillus niger using banana(Musa balbisiana)peel as substrate and its effect on clarification of banana juice[J].Journal of Food Science and Technology,2015,52(6):3 579-3 589.
[70] ANAND G,YADAV S,YADAV D.Production,purification and biochemical characterization of an exo-polygalacturonase from Aspergillus niger MTCC 478 suitable for clarification of orange juice[J].3 Biotech,2017,7(2):122.
[71] DE LIMA DAMSIO A R,DA SILVA T M,MALLER A,et al.Purification and partial characterization of an exo-polygalacturonase from Paecilomyces variotii liquid cultures[J].Applied Biochemistry and Biotechnology,2010,160(5):1 496-1 507.
[72] MARTINS EDA S,LEITE R S,DA SILVA R,et al.Purification and properties of polygalacturonase produced by thermophilic fungus Thermoascus aurantiacus CBMAI-756 on solid-state fermentation[J].Enzyme Research,2013,2 013:1-8.
[73] TOUNSI H,HADJ SASSI A,BEN ROMDHANE Z,et al.Catalytic properties of a highly thermoactive polygalacturonase from the mesophilic fungus Penicillium occitanis and use in juice clarification[J].Journal of Molecular Catalysis B:Enzymatic,2016,127:56-66.
[74] MA Yu-ping,HAO Hui,CHEN Zhi-fei,et al.Production,purification and physicochemical properties of an exo-polygalacturonase from Aspergillus niger SW06[J].Journal of the Brazilian Chemical Society,2016,28(4):622-628.
[75] JADHAV S R,PATHAK A P.Production and characterization of a thermo-pH stable pectinase from Bacillus licheniformis UNP-1:A novel strain isolated from Unapdev hot spring[J].Indian Journal of Geo Marine Sciences,2019,48(5):670-677.
[76] ANAND G,YADAV S,YADAV D.Purification and biochemical characterization of an exo-polygalacturonase from Aspergillus flavus MTCC 7589[J].Biocatalysis and Agricultural Biotechnology,2017,10:264-269.
[77] BEKLI S,AKTAS B,GENCER D,et al.Biochemical and molecular characterizations of a novel pH-and temperature-stable pectate lyase from Bacillus amyloliquefaciens S6 for industrial application[J].Molecular Biotechnology,2019,61(9):681-693.
[78] HOSSEINI S S,KHODAIYAN F,MOUSAVI S M E,et al.Immobilization of pectinase on the glass bead using polyaldehyde kefiran as a new safe cross-linker and its effect on the activity and kinetic parameters[J].Food Chemistry,2020,309:125777.
[79] DENG Zi-yu,WANG Fang,ZHOU Bin,et al.Immobilization of pectinases into calcium alginate microspheres for fruit juice application[J].Food Hydrocolloids,2019,89:691-699.
[80] SOJITRA U V,NADAR S S,RATHOD V K.Immobilization of pectinase onto chitosan magnetic nanoparticles by macromolecular cross-linker[J].Carbohydrate Polymers,2017,157:677-685.
[81] NADAR S S,RATHOD V K.A co-immobilization of pectinase and cellulase onto magnetic nanoparticles for antioxidant extraction from waste fruit peels[J].Biocatalysis and Agricultural Biotechnology,2019,17:470-479.
[82] FANG Gang,CHEN Hong-gao,ZHANG Yun-peng,et al.Immobilization of pectinase onto Fe3O4@SiO2-NH2 and its activity and stability[J].International Journal of Biological Macromolecules,2016,88:189-195.
[83] DAL MAGRO L,DE MOURA K S,BACKES B E,et al.Immobilization of pectinase on chitosan-magnetic particles:Influence of particle preparation protocol on enzyme properties for fruit juice clarification[J].Biotechnology Reports,2019,24:1-11.
[84] DE OLIVEIRA R L,DIAS J L,DA SILVA O S,et al.Immobilization of pectinase from Aspergillus aculeatus in alginate beads and clarification of apple and umbu juices in a packed bed reactor[J].Food and Bioproducts Processing,2018,109:9-18.
[85] GUO Yin-zhou,LIU Jiang-tao,ZHANG Ke-hu,et al.Synthesis of stimuli-responsive support material for pectinase immobilization and investigation of its controllable tailoring of enzymatic activity[J].Biochemical Engineering Journal,2017,121:188-195.
[86] ABDEL WAHAB W A,KARAM E A,HASSAN M E,et al.Optimization of pectinase immobilization on grafted alginate-agar gel beads by 2(4)full factorial CCD and thermodynamic profiling for evaluating of operational covalent immobilization[J].International Journal of Biological Macromolecules,2018,113:159-170.
[87] ALAGÖZ D,TUKEL S S,YILDIRIM D.Immobilization of pectinase on silica-based supports:Impacts of particle size and spacer arm on the activity[J].International Journal of Biological Macromolecules,2016,87:426-432.
[88] REHMAN H U,AMAN A,ZOHRA R R,et al.Immobilization of pectin degrading enzyme from Bacillus licheniformis KIBGE IB-21 using agar-agar as a support[J].Carbohydrate Polymers,2014,102:622-626.
[89] MAHMOUD K F,ABO-ELMAGD H I,HOUSSEINY M M.Micro-and nano-capsulated fungal pectinase with outstanding capabilities of eliminating turbidity in freshly produced juice[J].Food Science and Technology International,2018,24(4):330-340.

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