Determination of vancomycin antibiotics residues in animal derived food by solid phase extraction and high performance liquid chromatography-tandem mass spectrometry

Authors

Jin LIN, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075 , China ；Hubei ProvincialEngineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075 , China
Xiao-long FAN, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075 , China ；Hubei ProvincialEngineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075 , China
Feng JIANG, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075 , China ；Hubei ProvincialEngineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075 , China
Qing-zhi PENG, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075 , China ；Hubei ProvincialEngineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075 , China
Tao-hong ZHOU, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430075 , China ；Hubei ProvincialEngineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430075 , China

Abstract

A accurate and quantitative method was developed for the determination of vancomycin antibiotics residues in animal food by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The samples were extracted by formic acid acetonitrile solution, degreased by acetonitrile-saturated n-hexane, purified by cation solid-phase extraction column, detected in the multi reaction monitoring (MRM) positive ion mode, and quantified by internal standard method using didechloro vancomycin as internal standard. The results showed that the target of vancomycin and norvancomycin had a good linear relationship in the range of 20~200 μg/L, and the correlation coefficient (R²) could reach more than 0.997. The average recoveries varied from 88.7% to 115.1% at the spiked levels of 20, 50 and 200 μg/kg, and the relative standard deviations were in the range of 3.1%~9.4%. The limit of detection (LOD) for the method was as low as 1.0 μg/kg, and limit of quantitation (LOQ) could reach 3.0 μg/kg. This method is rapid, sensitive, accurate, reproducible and suitable for the determination of vancomycin antibiotics residues in animal food.

Publication Date

2-18-2023

First Page

67

Last Page

71

DOI

10.13652/j.issn.1003-5788.2020.09.011

Recommended Citation

LIN, Jin; FAN, Xiao-long; JIANG, Feng; PENG, Qing-zhi; and ZHOU, Tao-hong (2023) "Determination of vancomycin antibiotics residues in animal derived food by solid phase extraction and high performance liquid chromatography-tandem mass spectrometry," Food and Machinery: Vol. 36: Iss. 9, Article 11.
DOI: 10.13652/j.issn.1003-5788.2020.09.011
Available at: https://www.ifoodmm.cn/journal/vol36/iss9/11

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