Whole genome analysis and molecular traceability study of Salmonella in fresh livestock and poultry meat

Authors

Sheng SONG, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha, Hunan 410117 ,China ；Hunan Institute of Food Quality Supervision Inspection and Research, Changsha, Hunan 410117 , China
Han GAO, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha, Hunan 410117 ,China ；Hunan Institute of Food Quality Supervision Inspection and Research, Changsha, Hunan 410117 , China
Li YAN, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha, Hunan 410117 ,China ；Hunan Institute of Food Quality Supervision Inspection and Research, Changsha, Hunan 410117 , China
Kun-peng GUO, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha, Hunan 410117 ,China ；Hunan Institute of Food Quality Supervision Inspection and Research, Changsha, Hunan 410117 , China
Hai-yun ZHANG, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha, Hunan 410117 ,China ；Hunan Institute of Food Quality Supervision Inspection and Research, Changsha, Hunan 410117 , China
Fang-bin WANG, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning, Changsha, Hunan 410117 ,China ；Hunan Institute of Food Quality Supervision Inspection and Research, Changsha, Hunan 410117 , China

Abstract

In order to investigate the contamination status of Sal-monella in fresh pork and fresh chicken, and research its whole genome characteristics and molecular traceability, the laboratory randomly collected 24 fresh pork and 27 fresh chicken from the fresh market in Changsha, were inspected according to “National Food Safety Standard Food microbiological examination: Salmonella” GB 4789.4—2016. The results showed that in 24 randomly selected fresh pork, 20 samples were detected, with the detection rate of 83.3%; in 27 randomly selected fresh chicken, 20 samples were detected, and the detected rate is 74.1%; the total detection rate was 78.4%. The results showed that the fresh pork and fresh chicken sold on the fresh market in Changsha were seriously contaminated by Salmonella, which had great risk for food safety. 40 of them were analyzed by whole genome sequencing and molecular traceability analysis. The results showed that 20 kinds of pig-derived Salmonella strains and 20 kinds of chicken-derived Salmonella strains could be divided into 5 clusters, and clearly reflected the distribution of pollution sources and cross-contamination of Salmonella, achieving precise traceability. 15 pathogenic genes were found, which clarified the possible pathogenic genes and pathogenic mechanisms on the genetic level, and suggested the potential risk of Salmonella. The research on the pathogenic mechanism of related diseases provided guidance for diseases control. 15 genes involved in drug-resistance were found, which clarified the possible drug-resistant genes and the relative mechanisms on the genetic level, providing important data for the control of highly drug-resistant strains.

Publication Date

2-18-2023

First Page

55

Last Page

62, 169

DOI

10.13652/j.issn.1003-5788.2020.09.009

Recommended Citation

SONG, Sheng; GAO, Han; YAN, Li; GUO, Kun-peng; ZHANG, Hai-yun; and WANG, Fang-bin (2023) "Whole genome analysis and molecular traceability study of Salmonella in fresh livestock and poultry meat," Food and Machinery: Vol. 36: Iss. 9, Article 9.
DOI: 10.13652/j.issn.1003-5788.2020.09.009
Available at: https://www.ifoodmm.cn/journal/vol36/iss9/9

References

[1] 韩荣伟,于忠娜,张莉,等.我国鸡肉产品中沙门氏菌风险评估的研究进展[J].食品科学,2015,36(23):372-376.
[2] 赵贵,张华.畜产品中沙门氏菌的危害及检测方法概述[J].贵州畜牧兽医,2004,28(3):21-22.
[3] 尹德凤,张莉,张大文,等.食品中沙门氏菌污染研究现状[J].江西农业学报,2015,27(11):55-60.
[4] BRADLEY L B,SHAWN M D Bearson.Host specific differences alter the requirement for certain Salmonella genes during swine colonization[J].Veterinary Microbiology,2011,150(3/4):215-219.
[5] 叶恒平,张雅婷,袁敏,等.2014—2018年仙桃市伤寒沙门菌分子分型及耐药性分析[J].国际检验医学杂志,2020,41(9):1 042-1 046.
[6] 施怡茹,赵锦江,徐秋芳,等.沙门菌血清型多态性与PFGE分型及其相关性研究[J].检验医学与临床,2020,17(14):2 017-2 019.
[7] 畅晓晖,张捷,陈广全,等.北京地区肉类中沙门氏菌全基因组分型及耐药分析[J].食品安全质量检测学报,2020,11(3):783-791.
[8] 杨晓雯.布鲁氏菌等三种兼性胞内寄生菌比较基因组分析及应用[D].北京:中国农业大学,2018:12-24.
[9] 厚华艳.肠炎沙门氏菌菌毛编码基因sefA和fimA的功能探析[D].扬州:扬州大学,2013:2-6.
[10] 何华,斐洁,吴斌,等.猪支气管败血波氏杆菌菌毛fimD基因的克隆表达及间接ELISA检测方法的建立[J].畜牧兽医学报,2009,40(1):98-102.
[11] 曹恬雪,蒋文灿,何文成,等.沙门氏菌毒力因子的研究进展[J].中国预防兽医学报,2014,36(4):331-334.
[12] 黄冠军,刘天强,杨晓玲,等.沙门氏菌入侵基因研究进展[J].亚太传统医药,2013,9(11):65-67.
[13] 王小元,宋鸿军.细菌内毒素的生物合成途径及分子结构多样性[J].食品与生物技术学报,2013,32(10):1 009-1 015.
[14] 董方.沙门氏菌外膜蛋白的研究[D].北京:中国食品药品检定研究院,2013:8-11.
[15] 汤佩佩.肠炎沙门菌sopE2与sptP基因缺失株的构建及其免疫生物学研究[D].扬州:扬州大学,2016:9-21.
[16] 李隽.系统性红斑狼疮生物标志物的筛选及致病机制的研究[D].北京:北京协和医学院,2017:42-72.
[17] 徐婷,金顺鑫,王潇,等.细菌外排泵系统acrAB-tolC的研究进展[J].黑龙江畜牧兽医,2016(9):92-95.
[18] 钭慧芬.三种菌ampG基因的结构及遗传互补功能研究[D].温州:温州医学院,2013:1-8.
[19] 许泽仰.甲型副伤寒沙门氏菌norRVW操纵子的功能研究[D].昆明:昆明理工大学,2016:15-22.
[20] 朱培,李鑫强,李振轮.磷酸酶在病原菌侵染寄主中的作用[J].生物工程学报,2012,28(2):154-163.
[21] 程序.万古霉素耐药靶酶VanX抑制剂及金属-β-内酰胺酶检测试剂Nitrocefin的合成与表征[D].西安:西北大学,2011:7-22.