Construction of lead (II) ion detection method based on regulation of thiacyanine self-assembly by nucleic acid aptamer

Authors

ZHAO Han, National Center for Occupational Safety and Health, NHC, Beijing 102308, China
YU Li-jia, National Center for Occupational Safety and Health, NHC, Beijing 102308, China
WU Zhi-sheng, Taizhou Medical New & Hi-tech Industrial Development Zone, Taizhou, Jiangsu 225300, China
ZHANG Ying-lin, National Center for Occupational Safety and Health, NHC, Beijing 102308, China
ZHANG Hua-wei, National Center for Occupational Safety and Health, NHC, Beijing 102308, China
SHI Xiao-dong, National Center for Occupational Safety and Health, NHC, Beijing 102308, China

Abstract

In this paper, regulation of self-assembly properties of thiacyanine dye (Dye2) by nucleic acid aptamer (T30695) was used to construct a specific recognition unit for detecting Pb²⁺. G-quadruplexes were formed to interact with dye 2 monomers, which could disassemble dimers of Dye2 into monomer, in the presence of Pb²⁺. The absorption vales at 422 nm via 445 nm was used as a specific recognition signal to achieve the purpose of specificity and high sensitivity identification of Pb²⁺ only by UV-vis spectra. This system has a good linear relationship from 0.25 to 10.00 μmol/L (R² = 0.992 7), and the detection limit is 0.115 μmol/L.

Publication Date

1-28-2021

First Page

73

Last Page

78

DOI

10.13652/j.issn.1003-5788.2021.01.011

Recommended Citation

Han, ZHAO; Li-jia, YU; Zhi-sheng, WU; Ying-lin, ZHANG; Hua-wei, ZHANG; and Xiao-dong, SHI (2021) "Construction of lead (II) ion detection method based on regulation of thiacyanine self-assembly by nucleic acid aptamer," Food and Machinery: Vol. 37: Iss. 1, Article 11.
DOI: 10.13652/j.issn.1003-5788.2021.01.011
Available at: https://www.ifoodmm.cn/journal/vol37/iss1/11

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