Study on the production of Fuqu and the characteristics of enzyme about a cellulase producing Bacillus subtilis

Authors

ZHENG Zi-qiang, School of Biological Engineering, Sichuan University of Technology, Yibin, Sichuan 644000, China; Sichuan University of Science & Engineering, Liquor Brewing Biotechnology and Application Key Laboratory of Sichuan Province, Yibin, Sichuan 644000, China
WEI Chun-hui, School of Biological Engineering, Sichuan University of Technology, Yibin, Sichuan 644000, China; Sichuan University of Science & Engineering, Liquor Brewing Biotechnology and Application Key Laboratory of Sichuan Province, Yibin, Sichuan 644000, China
DENG Jie, School of Biological Engineering, Sichuan University of Technology, Yibin, Sichuan 644000, China; Sichuan University of Science & Engineering, Liquor Brewing Biotechnology and Application Key Laboratory of Sichuan Province, Yibin, Sichuan 644000, China
HUANG Zhi-guo, School of Biological Engineering, Sichuan University of Technology, Yibin, Sichuan 644000, China; Sichuan University of Science & Engineering, Liquor Brewing Biotechnology and Application Key Laboratory of Sichuan Province, Yibin, Sichuan 644000, China
ZHONG Ji-yu, School of Biological Engineering, Sichuan University of Technology, Yibin, Sichuan 644000, China
REN Zhi-qiang, School of Biological Engineering, Sichuan University of Technology, Yibin, Sichuan 644000, China; Sichuan University of Science & Engineering, Liquor Brewing Biotechnology and Application Key Laboratory of Sichuan Province, Yibin, Sichuan 644000, China

Abstract

Objective: In order to develop and utilize the resource utilization rate of cellulase producing strains. Methods: A Bacillus subtilis XWS-A with complete cellulase production system and high activity was used as the starting strain to make bacterial Fuqu. Box-Behnken response surface design was used to optimize the technology of Fuqu, and its enzyme production characteristics were explored. Results: The optimum conditions of Fuqu making were fixed inoculum amount of 7.5%, culture temperature of 46 ℃, water content of 46%, Under these conditions, Bacillus subtilis XWS-A was effectively proliferated, and the biomass reached （4.5±0.3）×10¹¹ CFU/g; Fuqu had three kinds of cellulase activities, and the best conditions for determination were endonuclease. The activity of endo-β-glucanase was （2 510±70） U/g. The activity of exo-β-glucanase was （15±2） U/g. The activity of β-glucosidase was （30±3） U/g. Conclusion: By making Fuqu, XWS-A can effectively proliferate and improve the enzyme production of the strain, and the Fuqu can maintain the enzyme activity of the strain. It provides an effective way for the efficient utilization of cellulase producing strains and the development and utilization of cellulose resources.

Publication Date

10-28-2021

First Page

12

Last Page

17

DOI

10.13652/j.issn.1003-5788.2021.10.003

Recommended Citation

Zi-qiang, ZHENG; Chun-hui, WEI; Jie, DENG; Zhi-guo, HUANG; Ji-yu, ZHONG; and Zhi-qiang, REN (2021) "Study on the production of Fuqu and the characteristics of enzyme about a cellulase producing Bacillus subtilis," Food and Machinery: Vol. 37: Iss. 10, Article 3.
DOI: 10.13652/j.issn.1003-5788.2021.10.003
Available at: https://www.ifoodmm.cn/journal/vol37/iss10/3

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