Abstract
Objective: This study focuses on obtaining a Lactobacillus strain for degrading triglyceride. Methods: Lactocillus was isolated from human breast milk and the faeces of healthy infants in this study, and their triglyceride degradability was determined by single reagent （GPO-PAP） method. The strains with strong degradation ability of triglyceride were selected for morphological, physiological, biochemical and 16S rDNA identification, and their tolerance to acid, bile salt and gastrointestinal juice was determined. Results: A strain of Lactobacillus Paracasei was isolated and then named as HCS17-040 （CGMCC No 19747）, and its degradation rate of triglyceride was （42.80±1.79）%; the survival rates of the strain were 89.35% and 87.18% after 17 h culture in pH 3.0 and pH 2.0, respectively, and then reached 94.75% and 92.97% after 17 h culture in 3 g/L and 5 g/L bile salts, respectively, the survival rate was 93.93% and 99.06% in artificial intestinal fluid for 4 hours. Conclusion: Lactobacillus Paracasei HCS17-040 has a certain ability to remove triglycerides, and has a good ability to resist acid, bile salt and artificial gastric and intestinal fluid.
Publication Date
11-28-2021
First Page
28
Last Page
33,129
DOI
10.13652/j.issn.1003-5788.2021.11.005
Recommended Citation
Ping, YU; Lan, CAO; Yan-ping, JIAO; Di, ZHAO; and Xiang-bo, MIN
(2021)
"Screening of Lactobacillus Paracasei HCS17-040 with triglyceride tunction and study on its probiotic characteristics,"
Food and Machinery: Vol. 37:
Iss.
11, Article 5.
DOI: 10.13652/j.issn.1003-5788.2021.11.005
Available at:
https://www.ifoodmm.cn/journal/vol37/iss11/5
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