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Abstract

Objective: A high-precision, low-cost quantitative and rapid detection method of nitrite in food based on photoelectric detection principle was proposed, and a portable nitrite detector equipped with microfluidic mixer chip was constructed. Methods: The microfluidic mixer chip was designed to realize the rapid mixing of test samples. The mixing efficiency of the chip was verified by peristaltic fluid simulation. The influence of UV curing parameters on the microfluidic mixer chip channel accuracy was analyzed, the microfluidic mixer chip was prepared. The photoelectric detection system of nitrite was designed and established. The relationship between nitrite content and induced voltage was analyzed, the detection sensitivity of the system was determined. Results: The detection limit of photoelectric detection system could reach 95.7 μg/L at a confidence factor of 3. The fitting error of the linear index of the system was 0.996 7, the average detection time was 3 min. Conclusion: The UV curing technology can realize the high-precision fabrication of microfluidic mixer chip. The detection accuracy and sensitivity of the self-developed photoelectric detection system can meet the requirements of nitrite detection in food.

Publication Date

11-28-2021

First Page

50

Last Page

54,158

DOI

10.13652/j.issn.1003-5788.2021.11.009

References

[1] 刘宁, 沈明浩. 食品毒理学[M]. 北京: 北京中国轻工业出版社, 2007: 261-262.
[2] 周毅文, 黄世杰, 吴俊星, 等. 食品中亚硝酸盐的测定方法研究进展[J]. 广州化工, 2019, 47(20): 29-31.
[3] 陈景周, 李樑, 黄少漫, 等. 高压离子色谱法快速测定饮用水中溴酸盐和亚硝酸盐[J]. 食品与机械, 2020, 36(7): 63-66.
[4] 李新民, 罗学科, 李文, 等. 基于颜色检测的亚硝酸盐传感器设计[J]. 北京化工大学学报(自然科学版), 2020, 47(6): 79-84.
[5] HOU C Y, FU L M, JU W J, et al. Microfluidic colorimetric system for nitrite detection in foods[J]. Chemical Engineering Journal, 2020, 398: 125 573-125 580.
[6] THONGKAM T, HEMAVIBOOL K. An environmentally friendly microfluidic paper-based analytical device for simultaneous colorimetric detection of nitrite and nitrate in foodproducts[J]. Microchemical Journal, 2020, 159: 105 412-105 419.
[7] 肖良品, 刘显明, 刘启顺, 等. 用于亚硝酸盐快速检测的三维纸质微流控芯片的制作[J]. 食品科学, 2013, 34(22): 341-345.
[8] 莫乃静. 浅谈食品中亚硝酸盐检测改进方法与结果比对分析[J]. 科技经济导刊, 2017(19): 128, 108.
[9] 刘超. 微流控芯片快速混合器及其应用研究[D]. 武汉: 华中科技大学, 2016: 26-36.
[10] 李勇, 王欣欣, 王瑞金. 影响微流体混合的因素及微混合器[J]. 新技术新工艺, 2008, 28(7): 40-45.
[11] ROESER H P, BOHR A, HASLAM D T, et al. Size quantization in high-temperature superconducting cuprates and a link to Einstein's diffusionlaw[J]. Acta Astronautica, 2012, 76: 37-41.
[12] ZENG Y, MORRIS J. Detection limits of optical gas imagers as a function of temperature differrential and distance[J]. Journal of the Air & Waste Management Association, 2019, 69(3): 351-361.
[13] 孟伟. 3D打印技术及应用趋势分析[J]. 科技创新与应用, 2021, 10(11): 146-148.
[14] 邵中魁, 姜耀林. 光固化3D打印关键技术研究[J]. 机电工程, 2015, 32(2): 180-184.
[15] 宗学文, 肖涵, 徐文博, 等. 基于面成型光固化3D打印制件的尺寸精度[J]. 工程塑料应用, 2018, 46(2): 41-46.
[16] 张炜. 运动饮料对不同光固化复合树脂颜色稳定性的影响[J]. 食品与机械, 2021, 37(1): 61-65.
[17] 崔丽佳, 王升高, 张维, 等. 玻璃-PDMS微流控芯片的电渗性能研究[J]. 真空与低温, 2016, 22(4): 201-204, 209.
[18] 王虹, 张朝纯, 孙洁浩, 等. α-氰基丙烯酸酯胶黏剂的应用与发展[J]. 精细与专用化学品, 2020, 28(12): 46-49.
[19] YU Bo, LI Yan, CHEN Zhi-hong. Photoelectric detection of hole shape and size for large plate parts[J]. Journal of Physics: Conference Series, 2021, 1 744(2): 22 119-22 125.
[20] 黄硕, 王彪, 戴童欣, 等. 用于CO激光TDLAS型气体检测系统的光电检测电路研制[J]. 激光杂志, 2021, 42(2): 62-65.
[21] 李兵, 孙长应, 李为宁, 等. 基于光电控制的六安瓜片烘焙机设计[J]. 食品与机械, 2018, 34(5): 106-109.
[22] 徐榕, 杨叶欣, 林敏, 等. 基于显色法的纸基微流控芯片用于水中的三氮检测[J]. 分析化学, 2020, 48(9): 1 202-1 209.
[23] 朱鸣, 张娅, 许姗, 等. 硫化亚锡—碳球复合物的制备及其在亚硝酸盐检测中的应用[J]. 扬州大学学报(自然科学版), 2019, 22(4): 21-25.
[24] FANG Teng-yue, LI Hang-qian, BO Guang-yong, et al. On-site detection of nitrate plus nitrite in natural water samples using smartphone-based detection[J]. Microchemical Journal, 2021, 165: 106 117-106 124.
[25] 商景天, 王修俊, 王继辉. 有机酸对泡菜中亚硝酸盐的降解作用[J]. 食品与机械, 2018, 34(3): 73-78.
[26] 李慧, 张子燕. 泡菜中亚硝酸盐的研究[J]. 现代食品, 2020, 21(20): 88-90.
[27] 易任远. 数码成像比色在“检测泡菜中亚硝酸盐含量”实验中的应用[J]. 生物学教学, 2020, 45(1): 48-50.

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