Abstract
Objective: The research aimed to optimize the supercritical CO2 extraction process of volatile oil from Perilla frutescens leaf and enhance the practical value of Perilla frutescens. Methods: The optimal parameters for extraction of volatile oil from Perilla frutescens leaf was predicted by single factor experiments （extraction pressure, extraction temperature, extraction time and CO2 flow） and response surface methodology. Then the gas chromatography-mass spectrometry （GC-MS） was applied to determine the chemical components of volatile oil from Perilla frutescens leaf. In addition, its antioxidant activity was explored with the scavenging capacity on DPPH· and ·OH radicals. Results: The optimal process parameters of supercritical CO2 extraction for volatile oil from Perilla frutescens leaf were founded to be at 27 MPa, 40 ℃ and 2 h, the actual yield was （2.71±1.19）%, which was 67% higher than the vapor distillation under the same extraction time and reduce the peroxide value and acid value. The 51 chemical compounds were identified in the volatile oil from Perilla frutescens leaf and accounted for 98.49% of all the peak area, which contained lipids, alcohols and olefins. The volatile oil of Perilla frutescens leaf had strong scavenging effect on DPPH· and ·OH radicals, and the scavenging capacity had a significant positive correlation with oil concentration. Conclusion: In this study, the supercritical CO2 extraction of volatile oil from Perilla frutescens leaf were mainly composed of lipids, alcohols and olefins, which has strong antioxidant capacity.
Publication Date
12-28-2021
First Page
142
Last Page
148
DOI
10.13652/j.issn.1003-5788.2021.12.024
Recommended Citation
Fu-you, QI; Shun-hua, JIAN; Yin, LIU; Li-ping, DUAN; and Zhao, WU
(2021)
"Extraction process optimization, composition analysis of volatile oil from Perilla frutescens leaf and its antioxidant activity,"
Food and Machinery: Vol. 37:
Iss.
12, Article 24.
DOI: 10.13652/j.issn.1003-5788.2021.12.024
Available at:
https://www.ifoodmm.cn/journal/vol37/iss12/24
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