Simultaneous determination of four flavonoid glycosides and limonin in pomelo by HPLC

Authors

ZHANG Shan, Food Science and Engineering, Jinan University, Guangzhou, Guangdong 510632, China
HUANG Xue-song, Food Science and Engineering, Jinan University, Guangzhou, Guangdong 510632, China

Abstract

In order to simultaneously determine narirutin, naringin, hesperidin, neohesperidin and limonin in pomelo, the effects of elution conditions and measurement wavelength of high-pressure liquid chromatography on the determination results were studied. The results showed that the optimal measurement conditions were as follows. Chromatographic column of Bio-Bond 5 μm C₁₈ (4.6 mm × 250 mm was used, and the injection volume was 10 μL; the detection wavelengths of flavonoid glycosides and limonin were 283 nm and 220 nm, respectively; The phase: included acetonitrile (A) and water (B) with gradient elution of 0 min, 22% A, 8 min, 22% A, 10 min, 95% A, 16 min, 95% A, 18 min, 22% A, and 20 min, 22% A, with 1 mL/min flow rate. Under the control o these conditions, the above five components were well separated; the correlation coefficient of the standard curve was greater than 0.999 7, and the detection limit was 0.69~1.72 mg/L. The quantification limit was 1.51~3.15 mg/L, and the relative standard deviation of repeatability was less than 2%. The sample recovery rates were all greater than 98%, and the relative standard deviation of the sample recovery rates were all less than 2%.

Publication Date

3-28-2021

First Page

58

Last Page

63

DOI

10.13652/j.issn.1003-5788.2021.03.011

Recommended Citation

Shan, ZHANG and Xue-song, HUANG (2021) "Simultaneous determination of four flavonoid glycosides and limonin in pomelo by HPLC," Food and Machinery: Vol. 37: Iss. 3, Article 11.
DOI: 10.13652/j.issn.1003-5788.2021.03.011
Available at: https://www.ifoodmm.cn/journal/vol37/iss3/11

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