Abstract
The residue of Passiflora edulis Sims leaves after hot water extraction was used as material. Polysaccharide was extracted from the residue by using NaOH alkaline solution, followed by separation of ion-exchange chromatography. The physicochemical properties of polysaccharide were determined and analyzed by IR, HPLC and gel permeation chromatography, and then its hypoglycemic and anticoagulant were studied. The results showed that APEL was separated by DEAE-52 cellulose ion-exchange chromatography to obtain the APEL-1, APEL-2, APEL-3, the APEL and its separated components all have typical characteristic peaks of polysaccharides, mainly composed of arabinose, glucose, galactose, xylose, rhamnose, glucuronic acid. The molecular weights of APEL, APEL-1, APEL-2 and APEL-3 were 4.277×106, 4.276×106, 4.203×106, 4.111×106 Da. The APEL and its components could significantly inhibit α-amylase and α-glucosidase, and prolong the activated partial thromboplastin time, prothrombin time and thrombin time, showing obvious hypoglycemic and anticoagulant activities in vitro.
Publication Date
3-28-2021
First Page
137
Last Page
143
DOI
10.13652/j.issn.1003-5788.2021.03.026
Recommended Citation
Xia, LI; Cheng-xin, LIU; Yan, HUANG; Guan-lan, MO; and Yuan, GUAN
(2021)
"Isolation, identification and its biological activity analysis of the polysaccharide in Passiflora edulis Sims leaves from alkali extracts,"
Food and Machinery: Vol. 37:
Iss.
3, Article 26.
DOI: 10.13652/j.issn.1003-5788.2021.03.026
Available at:
https://www.ifoodmm.cn/journal/vol37/iss3/26
References
[1] 朱文娴, 夏必帮, 廖红梅. 西番莲的功能活性成分及加工与综合利用研究进展[J]. 食品与机械, 2018, 34(12): 181-184.
[2] 周正元, 程昊, 唐婷范, 等. 百香果叶总黄酮提取工艺优化[J]. 广西科技大学学报, 2019, 30(3): 115-119.
[3] COLOMEU T C, FIGUEIREDO D, CAZARIN C B, et al. Antioxidant and anti-diabetic potential of Passiflora alata curtis aqueous leaves extract in type 1 diabetes mellitus (nod-mice)[J]. International Immunopharmacology, 2014, 18(1): 106-115.
[4] 全娜. 枸杞叶多糖的分离纯化、结构表征及降血糖活性分析[D]. 西安: 陕西师范大学, 2018: 73-74.
[5] 马琳. 碱提枣多糖抗凝血作用途径的初步探讨[D]. 郑州: 河南农业大学, 2014: 41-42.
[6] FRANCISCO D S C, GLAUBER C L, VALESCA I N S, et al. Sulfated polysaccharide from the red algae Gelidiella acerosa: Anticoagulant, antiplatelet and antithrombotic effects[J]. International Journal of Biological Macromolecules, 2020, 159: 415-421.
[7] 李霞, 熊峰, 覃献杏, 等. 西番莲果皮多糖微波辅助提取工艺优化及其体外抗氧化性[J]. 食品工业科技, 2018, 39(15): 141-146.
[8] 李霞, 胡楠, 陈齐琳, 等. 西番莲叶多糖提取工艺的优化及其体外抗氧化活性研究[J]. 中国调味品, 2020, 45(10): 153-157, 162.
[9] LWEIS B J, HERRLINGER K A, CRAIG T A, et al. Antihypertensive effect of passion fruit peel extract and its major bioactive components following acute supplementation in spontaneously hypertensive rats[J]. The Journal of Nutritional Biochemistry, 2013, 24(7): 1 359-1 366.
[10] 焦中高, 张春岭, 刘杰超, 等. 碱提红枣多糖与水提红枣多糖生物活性的比较研究[J]. 食品安全质量检测学报, 2015, 6(10): 4 181-4 187.
[11] MA Jin-shuang, LIU Hong, HAN Chang-ri, et al. Characterization and antioxidant activity of polysaccharide from Pouteria campechiana seed[J]. Carbohydrate Polymers, 2020, 229: 115409.
[12] 白丽丹, 段懿涵, 谭超杰, 等. 蛹、米虫草多糖含量及抗氧化活性比较研究[J]. 食品研究与开发, 2020, 41(20): 22-29.
[13] NELLY B, GUSTAV A H. New method for quantitative determination of uronic acids[J]. Analytical Biochemistry, 1973, 54(2): 484-489.
[14] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemi-stry, 1976, 72(1/2): 248-254.
[15] 谢静静, 付文鹏, 赵艳雯, 等. 美洲大蠊脱脂膏中总糖和还原糖含量的测定[J]. 食品工业科技, 2019, 40(3): 199-206.
[16] 黄小兰, 何旭峰, 杨勤, 等. PMP柱前衍生化HPLC法测定地参多糖的单糖组成[J]. 食品与发酵工业, 2020, 46(7): 250-256.
[17] TRUC C H, ANLEY T K, SARAVANA P S, et al. Green extraction of polyphenolic-polysaccharide conjugates from Pseuderanthemum palatiferum (Nees) Radlk: Chemical profile and anticoagulant activity[J]. International Journal of Biological Macromolecules, 2020, 157: 484-493.
[18] CHEN Chun, ZHANG Bin, HUANG Qiang, et al. Microwave-assisted extraction of polysaccharides from Moringa oleifera Lam. leaves: Characterization and hypoglycemic activity[J]. Industrial Crops & Products, 2017, 100: 1-11.
[19] XIAO Heng, FU Xiong, CAO Chang-liang, et al. Sulfated modification, characterization, antioxidant and hypoglycemic activities of polysaccharides from Sargassum pallidum[J]. International Journal of Biological Macromolecules, 2018, 121: 407-414.
[20] 孔艳, 禤日翔, 戴梓茹, 等. 近江牡蛎糖胺聚糖降血糖及抗凝血活性研究[J]. 食品科技, 2019, 44(10): 289-293.
[21] GAO Han, ZHANG Wen-cheng, WU Ze-yu, et al. Preparation, characterization and improvement in intestinal function of olysaccharide fractions from okra[J]. Journal of Functional Foods, 2018, 50: 147-157.
[22] 郭金英, 朱蓓茹, 任国艳, 等. 发菜胞外多糖硫酸化条件的优化及红外光谱分析[J]. 食品科学, 2016, 37(24): 61-67.
[23] 王莹, 申秀娟, 张磊, 等. 火炬树芽多糖提取工艺的响应面优化及红外光谱分析[J]. 粮食与油脂, 2019, 32(9): 96-100.
[24] 刁毅, 刘涛, 韩洪波. 不同地区地木耳多糖红外光谱与抗氧化活性研究[J]. 湖北农业科学, 2016, 55(4): 984-987, 996.
[25] 马天晓, 尹震花, 张翠利, 等. 流苏子多糖的提取分离、理化性质及体外α-葡萄糖苷酶抑制活性[J]. 食品工业科技, 2020, 41(24): 64-68, 80.
[26] IZABELA P, LESZEK C, WITOLD P, et al. Polyphenolic-polysaccharide compounds from selected medicinal plants of Asteraceae and Rosaceae families: Chemical characterization and blood anticoagulant activity[J]. Carbohydrate Polymers, 2009, 77(3): 568-575.
[27] LI Shi-jie, XIONG Qing-ping, LAI Xiao-ping, et al. Molecular modification of polysaccharides and resulting bioactivities[J]. Com Prehensive Reviews in Food Science and Food Science and Food Safety, 2016, 15(2): 237-250.
[28] 卞春, 王振宇, JOHN S, 等. 非肝素类天然抗凝血多糖的研究进展[J]. 食品工业科技, 2019, 40(18): 300-308.
[29] LI Hong-yan, MAO Wen-juan, ZHANG Xiu-li, et al. Structural characterization of an anticoagulant-active sulfated polysaccharide isolated from green alga Monostroma latissimum[J]. Carbohydrate Polymers, 2011, 85(2): 394-400.
[30] 崔莹莹, 张剑韵, 张容鹄, 等. 大蒜多糖的体外抗凝血作用及结构分析[J]. 食品与发酵工业, 2009, 35(4): 24-27.