Abstract
By measuring the yield of parvalbum, a kind of extraction solution of parvalbumin from Pseudosciaena crocea was screened out, and the relative extracting process was optimized. The results showed that when the extraction time was 42 h, the extract concentration was 72 mmol/L; the pH of the extract was 8, and the ratio of material to liquid was 1∶180 (g/mL); the extraction temperature was 50 ℃, and the extraction rate of parvalbumin reached 0.174 2%. Parvalbumin was then separated and purified by anion gel column chromatography, and its molecular weight was determined to be 12 kDa by Western blot analysis.
Publication Date
3-28-2021
First Page
150
Last Page
156,179
DOI
10.13652/j.issn.1003-5788.2021.03.028
Recommended Citation
Chun-xia, LU; Liu-ming, YANG; Shi-da, CHEN; Hui-en, ZHANG; and Hua, YANG
(2021)
"Optimization of the extraction process of parvalbumin from Pseudosciaena crocea,"
Food and Machinery: Vol. 37:
Iss.
3, Article 28.
DOI: 10.13652/j.issn.1003-5788.2021.03.028
Available at:
https://www.ifoodmm.cn/journal/vol37/iss3/28
References
[1] 李婷婷. 大黄鱼生物保鲜技术及新鲜度指示蛋白研究[D]. 杭州: 浙江工商大学, 2013: 1.
[2] 刘家富, 韩坤煌. 我国大黄鱼产业的发展现状与对策[J]. 福建水产, 2011, 33(5): 4-8.
[3] 张永霞, 杨阳, 蔡秋凤, 等. 水产食物过敏原及其抗原表位的研究进展[J]. 中国渔业质量与标准, 2015, 5(5): 1-8.
[4] SHRUTI R S, MICHAEL F S, SANDIP D K, et al. Antibody reactivity to the major fish allergen parvalbumin is determined by isoforms and impact of thermal processing[J]. Food Chemistry, 2014, 148: 321-328.
[5] SYED Hasan Arif. A Ca2+-binding protein with numerous roles and uses: Parvalbumin in molecular biology and physiology[J]. Bioessays, 2009, 31(4): 410-421.
[6] ANNETTE Kuehn, LNES Swoboda, KARTHIK Arumugam, et al. Fish allergens at a glance: Variable allergenicity of parvalbumins, the major fish allergens[J]. Frontiers in Immunology, 2014, 5: 179.
[7] ELSAYED S, AAS K, SLETTEN K, et al. Tryptic cleavage of a homogeneous cod fish allergen and isolation of two active polypeptide fragments[J]. Immunochemistry, 1972, 9(6): 647.
[8] AUKRUST L, APOLD J, ELSAYED S, et al. Crossed immunoelectrophoretic and crossed radioimmunoelectrophoretic studies employing a model allergen from codfish[J]. International Archives of Allergy & Immunology, 1978, 57(3): 253-262.
[9] ELSAYED S, AAS K. Isolation of purified allergens (cod) by isoelectric focusing[J]. International Archives of Allergy and Immunology, 1971, 40(3): 428-438.
[10] KOBAYASHI A, TANAKA H, HAMADA Y, et al. Comparison of allergenicity and allergens between fish white and dark muscles[J]. Allergy, 2006, 61(3): 357-363.
[11] JENNIFER L W, NISREEN M M, DAVID J. Coughlin Parvalbumin correlates with relaxation rate in the swimming muscle of sheepshead and kingfish[J]. Journal of Experimental Biology, 2006, 209(2): 227-237.
[12] SWOBODA L, BUGAJSKASCHRETTER A, LINHART B, et al. A recombinant hypoallergenic parvalbumin mutant for immunotherapy of IgE-mediated fish allergy[J]. Journal of Immunology, 2007, 178(10): 6 290-6 296.
[13] 郭岩. 鱼类主要过敏原小清蛋白的单克隆抗体制备及其金磁酶联免疫体系的构建[D]. 上海: 上海师范大学, 2017: 4.
[14] 陆宗超. 大菱鲆过敏原小清蛋白的制备及氧化对其IgE结合能力的影响[D]. 北京: 中国海洋大学, 2013.
[15] 蔡秋凤, 刘光明, 王瑞芳. 鱼类主要过敏原小清蛋白的检测[J]. 中国免疫学杂志, 2010, 26(8): 716-720, 723.
[16] 肖有明, 刘红, 陈红兵, 等. 鲤鱼小清蛋白过敏原的分离纯化[J]. 食品科学, 2008, 29(12): 385-388.
[17] 吴玟静, 李振兴, 金科, 等. 青岛地区常见鱼类过敏原的鉴定及其交叉反应研究[J]. 水产学报, 2011, 35(10): 1 532-1 539.
[18] MOHAMED F J, THOMAS G R, MARY E M, et al. Occupational allergy and asthma among salt water fish processing workers[J]. American Journal of Industrial Medicine, 2008, 51(12): 899-910.
[19] 夏珊珊, 曹少谦, 刘亮, 等. 响应面法优化马面鱼皮胶原蛋白提取工艺及胶原组成分析[J]. 核农学报, 2014, 28(5): 869-875.
[20] 田家亮, 周宗科, 廉永云, 等. 三种不同脱脂方法对猪骨脱脂的效应[J]. 中国组织工程研究与临床康复, 2009, 13(16): 3 133-3 136.
[21] 吴靖娜, 许永安, 刘智禹, 等. 养殖大黄鱼鱼片脱脂工艺研究[J]. 河南工业大学学报(自然科学版), 2013, 34(6): 27-31.
[22] 李晓, 李红艳, 王颖, 等. 响应面法优化狭鳕鱼鱼皮酸溶性胶原蛋白质的提取工艺[J]. 广西科学院学报, 2016, 32(4): 250-257.
[23] 李莉, 顾赛麒, 王锡昌, 等. 响应面法优化酶法提取大鲵皮胶原蛋白工艺[J]. 中国水产科学, 2013, 20(4): 876-883.
[24] 刘光明, 梁银龙, 苏文金, 等. 鲤鱼小清蛋白的纯化及其过敏原性鉴定[J]. 食品科学, 2009, 30(3): 188-191.
[25] ULRICH K Laemmli. Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J]. nature, 1970, 227(5 259): 680.
[26] KANNA Motoyama, YOTA Suma, SHOICHIRO Ishizaki, et al. Molecular cloning of tropomyosins identified as allergens in six species of crustaceans[J]. J Agric Food Chem, 2012, 55(3): 985-991.
[27] CHEN Ling-yun, SUE L Hefle, STEVE L Taylor, et al. Detecting fish parvalbumin with commercial mouse monoclonal anti-frog parvalbumin IgG[J]. Journal of Agricultural and Food Chemistry, 2006, 54(15): 5 577-5 582.
[28] 洪晶, 陈涛涛, 唐梦茹, 等. 响应面法优化韭菜籽蛋白质提取工艺[J]. 中国食品学报, 2013, 13(12): 89-96.
[29] 孙妙. 鱼过敏原的肠道可控释放及诱发免疫耐受的机制[D]. 北京: 中国海洋大学, 2013: 16-18.
[30] 李铮. 草鱼主要过敏原小清蛋白亚型纯化鉴定及加工对过敏原影响的研究[D]. 北京: 中国农业大学, 2014: 17-18.