•  
  •  
 

Abstract

The subcellular distribution of aluminium in seaweed and tea leaves was analyzed by differential centrifugation with microwave digestion and inductively coupled plasma mass spectrometer. The results showed that the proportion of aluminium in cell wall, organelle and cytoplasm of tea leaves were 78.4%, 11.2% and 9.5%, respectively; the proportion of aluminium in cell wall, organelle and cytoplasm of seaweed were 91.60%, 6.95% and 1.33%, respectively. Using transmission electron microscope with energy dispersive spectroscopy, surface scanning analysis was carried out on the distribution of aluminum in the single cell of tea leaves and seaweed, intuitively revealing that aluminum was mainly distributed in the cell wall of tea leaves and seaweed. The extraction of aluminium from tea leaves, seaweed and its subcellular components by simulated gastric juice was studied. The extraction rate of aluminium in tea leaves was about 9.0%, but the extraction rate of its subcellular components was less than 3.0%. The extraction rate of aluminum in seaweed and its subcellular components was less than 1.5%, suggesting that the food safety risk of ingestion of aluminum in tea and seaweed was low.

Publication Date

3-28-2021

First Page

46

Last Page

50,63

DOI

10.13652/j.issn.1003-5788.2021.03.009

References

[1] 渠心静, 陈雪燕, 戴生玉, 等. 22种山茶科植物叶片铝含量及其亚细胞分布[J]. 分子植物育种, 2018, 16(3): 997-1 006.
[2] 尚德荣, 赵艳芳, 宁劲松, 等. 海藻中铝的化学形态分析[J]. 水产学报, 2011, 35(4): 539-542.
[3] 李佳禾. 2019中国茶叶消费市场报告[J]. 茶世界, 2019(11): 43-51.
[4] YOKEL R A, FLORENCE R L. Aluminum bioavailability from tea infusion[J]. Food & Chemical Toxicology, 2008, 46(12): 3 659-3 663.
[5] WU Jun, ZHOU Chao-yan, WONG M K, et al. Urine levels of aluminum after drinking tea[J]. Biological Trace Element Research, 1997, 33(8): 1 213-1 219.
[6] GLYNN A W, SPAREN A, DANIELSSON L G, et al. Bioavailability of labile aluminium in acidic drinking water: A study in the rat[J]. Food & Chemical Toxicology An International Journal Published for the British Industrial Biological Research Association, 1995, 33(5): 403.
[7] 杨小弟, 章福平, 王先龙, 等. 环境与生物体系中铝形态分析技术的新进展[J]. 分析化学, 2003(9): 1 131-1 138.
[8] 黄国伟, 张文治. 铝对体外人胚大脑神经细胞毒作用的研究[J]. 中华预防医学杂志, 2000, 15(3): 791-795.
[9] BUSHINSKY D A, SPRAGUE S M, HALLEGOT P, et al. Effects of aluminum on bone surface ion composition[J]. Journal of Bone & Mineral Research, 2010, 10(12): 1 988-1 997.
[10] YOICHI H, TSUGUHISA F, KEISUKE Y, et al. Localization of aluminum in epidermal cells of mature tea leaves[J]. Quantum Beam Sci, 2019, 3(9): 1 365-1 371.
[11] ROSER T, VOGEL-MIKU K, HAJIBOLAND R, et al. Localization of aluminium in tea (Camellia sinensis) leaves using low energy X-ray fluorescence spectro-microscopy[J]. Journal of Plant Research, 2011, 124(1): 165-172.
[12] CARR H P, LOMBI E K, WONG M H, et al. Accumulation and distribution of aluminium and other elements in tea (Camellia sinensis) leaves[J]. Agronomie, 2003, 23(6): 705-710.
[13] GAO Hong-jian, ZHAO Qiang, ZHANG Xian-chen, et al. Localization of fluoride and aluminum in subcellular fractions of tea leaves and roots[J]. Agric Food Chem, 2014, 62(10): 2 313-2 319.
[14] LEONARD R T, HODGES D H K. Membrane-bound adenosine triphosphatase activities of oat roots[J]. Plant Physiology, 1973, 51(4): 749-754.
[15] HAUSLER E, PETERSEN M, ALFERMANN A W. Isolation of protoplasts and vacuoles from cell suspension cultures of Coleus blumei Benth[J]. Plant Cell Reports, 1993, 12(9): 510-512.
[16] IRAKI N M, BRESSAN R A, HASEGAWA P M, et al. Alteration of the physical and chemical structure of the primary cell wall of growth-limited plant cells adapted to osmotic stress[J]. Plant Physiology, 1989, 91(1): 39-47.
[17] ZHONG Hai-lin, LAUCHLI A. Changes of cell wall composition and polymer size in primary roots of cotton seedlings under high salinity[J]. Journal of Experimental Botany, 1993(4): 4.
[18] 王媛莉, 李梅青, 董明, 等. 草酸铵超声辅助提取豆腐柴果胶[J]. 食品科学, 2011(10): 88-91.
[19] XIE Zhong-lei, CHEN Zhuo, SUN Wen-tian, et al. Distribution of aluminum and fluoride in tea plant and soil of teagarden in central and southwest China[J]. Chinese Geographical Science, 2007, 17: 376-382.
[20] RENGEL Z, REID R J. Uptake of Al across the plasma membrane of plant cells[J]. Plant & Soil, 1997, 192(1): 31-35.
[21] CHANG Y C, YAMAMOTO Y, MATSUMOTO H. Accumulation of aluminium in the cell wall pectin in cultured tobacco (Nicotiana tabacum L.) cells treated with a combination of aluminium and iron[J]. Plant Cell & Environment, 2010, 22(8): 1 009-1 017.
[22] WALLACE G, FRY S. Phenolic components of the plant cellwall[J]. International Review of Cytology, 1994, 151: 229-267.
[23] STRACK D, JRGEN H, MMKEN M, et al. Cell wall-conjugated phenolics from coniferae leaves[J]. Phytochemistry, 1988, 27(11): 3 517-3 521.
[24] DREWITT P N, BUTTERWORTH K R, SPRINGALL C D, et al. Plasma levels of aluminium after tea ingestion in healthy volunteers[J]. Food and Chemical Toxicology, 1993, 31(1): 19.
[25] FAIRWEATHER-TAIT S J, PIPER Z, FATEMI S J A, et al. The effect of tea on iron and aluminium metabolism in the rat[J]. British Journal of Nutrition, 1991, 65(1): 61-68.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.