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Abstract

A new process for subcritical extraction of Cucumariafrondosa oil was established using freeze-dried Cucumariafrondosa visceral which is rich in lipid active substances. The subcritical extraction conditions were optimized by single factor and response surface methodology, and the best extraction process was obtained: material-liquid ratio 1∶3 (g/mL), extraction pressure 0.4 MPa, extraction times 5 times, extraction time 56.18 min, extraction temperature 48.55 ℃, extraction rate 23.38%. By comparing the physical and chemical indexes of oil extracted by different methods and gas chromatography-mass spectrometry (GC-MS) graphs, the physicochemical indexes of oil extracted by subcritical method are better than other methods, the content of EPA in the oil reaches 40.7%, indicating that the subcritical method for extracting Cucumariafrondosa oil has high process efficiency and more stable oil quality.

Publication Date

4-28-2021

First Page

165

Last Page

170

DOI

10.13652/j.issn.1003-5788.2021.04.031

References

[1] 徐梦豪, 侯召华, 林荣芳, 等. 冰岛刺参抗癌物质Frondoside A的研究进展[J]. 特产研究, 2020, 42(5): 71-77.
[2] TIPPER J P, LYONS-LEVY G, ATKINSON M A L, et al. Purication, characterization and cloning of tensilin, the collagen-bril binding and tissue-stiffening factor from Cucumariafrondosa dermis[J]. Matrix Biology, 2002, 21(8): 625-635.
[3] KALE V, FREYSDOTTIR J, PAULSEN B S, et al. Sulphated polysaccharide from the sea cucumber Cucumariafrondosa affect maturation of human dendritic cells and their activation of allogeneic CD4(+) T cells in vitro[J]. Bioactive Carbohydrates and Dietary Fibre, 2013, 2(2): 108-117.
[4] USTYUZHANINA N E, BILAN M I, DMITRENOK A S, et al. The structure of a fucosylated chondroitin sulfate from the sea cucumber Cucumariafrondosa[J]. Carbohydrate Polymers, 2017, 165: 7-12.
[5] YAYLI N. Minorsaponins from the sea cucumber Cucumariafrondosa[J]. Indian Journal of Chemistry, 2001, 40(5): 399-404.
[6] MA X, KUNDU N, COLLIN PD, et al. Frondoside A inhibits breast cancer metastasis and antagonizes prostaglandin E receptors EP4 and EP2[J]. Breast Cancer Res Treat, 2012, 132(3): 1 001-1 008.
[7] LIU Xiao-xiao, HAO Jie-jie, SHAN Xin-di, et al. Antithrombotic activities of fucosylated chondroitin sulfates and their depolymerized fragments from two sea cucumbers[J]. Carbohydrate Polymers, 2016, 152: 343-350.
[8] STEFANIAK-VIDARSSON M M, KALE V A, GUDJNSDTTIR M, et al. Bioactive effect of sulphated polysaccharidesderivedfromorange-footedseacucumber(Cucumariafrondosa)towardTHP-1macrophages[J]. Bioactive Carbohydrates and Dietary Fibre, 2017, 12: 14-19.
[9] HU Shi-wei, XU Lei-lei, SHI Di, et al. Eicosapentaenoic acid-enriched phosphatidylcholine isolated from Cucumariafrondosa exhibits anti-hyperglycemic effects via activating phosphoinositide 3-kinase/protein kinase B signal pathway[J]. Journal of Bioscience and Bioengineering, 2014, 117(4): 457-463.
[10] AMININ D L, AGAFONOVA I G, KALININ V I, et al. Immunomodulatory properties of frondoside A, a major triterpene glycoside from the North Atlantic commercially harvested sea cucumber Cucumariafrondosa[J]. Journal of Medicinal Food, 2008, 11(3): 443-453.
[11] 白雅婷. 海参性腺酶解产物对实验动物肠道微生物的影响[D]. 厦门: 集美大学, 2018: 1-2.
[12] 李连舫. 盐渍海参肠加工技水的要点[J]. 海洋渔业, 1988(5): 220.
[13] GIANASI B L, PARRISH C C, HAMEL J F, et al. Inuence of diet on growth, reproduction and lipid and fatty acid composition in the sea cucumber Cucumariafrondosa[J]. Aquaculture Research, 2017, 48(7): 3 413-3 432.
[14] ZHONG Y, KHAN M A, SHAHIDI F. Compositional characteristics and antioxidant properties of fresh and processed sea cucumber (Cucumariafrondosa)[J]. Journal of Agricultural and Food Chemistry, 2007, 55(4): 1 188-1 192.
[15] MAMELONA J, SAINT-LOUIS R. Proximate composition and nutritional prole of by-products from green urchin and Atlantic sea cucumber processing plants[J]. International Journal of Food Science and Technology, 2010, 45(10): 2 119-2 126.
[16] 朱新亮, 祁鲲. 亚临界流体萃取实验室成套设备研究现状及其应用[C]// 首届中国亚临界生物萃取技术发展论坛论文集. 安阳: 中国国际科技促进会, 2016: 81-87.
[17] 廉桂芳. 鱿鱼肝脏鱼油抗衰老及预防肝损伤功能评价[D]. 重庆: 西南大学, 2018: 14.

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