Antioxidant and antibacterial activities of different polar parts from Manilkara zapota leaves

Authors

MA Fei-yue, South Subtropical Crops Research Institute of the Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524091, China; Key Laboratory of Tropical Fruit Biology of the Ministry of Agriculture, Zhanjiang, Guangdong 524091, China
ZHANG Ming, South Subtropical Crops Research Institute of the Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524091, China; Key Laboratory of Tropical Fruit Biology of the Ministry of Agriculture, Zhanjiang, Guangdong 524091, China
LI Ya, South Subtropical Crops Research Institute of the Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524091, China; Key Laboratory of Tropical Fruit Biology of the Ministry of Agriculture, Zhanjiang, Guangdong 524091, China
DU Li-qing, South Subtropical Crops Research Institute of the Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524091, China; Key Laboratory of Tropical Fruit Biology of the Ministry of Agriculture, Zhanjiang, Guangdong 524091, China
SHUAI Xi-xiang, State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China

Abstract

In order to investigate the antioxidant and antibacterial activity of different polar parts (petroleum ether part, ethyl acetate part, n-butanol part and water part) from Manilkara zapota leaves. The DPPH radical-scavenging assay, ABTS radical-scavenging assay and FRAP antioxidant capacity assay were carried out. And the antibacterial effects of different polar parts from Manilkara zapota leaves against Bacilus subtillis, Escherichia coli and Staphylococcus aureus were studied. The results showed that the extracts obtained from ethyl acetate extraction had the highest antioxidant activities; the IC₅₀ of the DPPH radical-scavenging and ABTS radical-scavenging were (0.019±0.010) mg/mL, and the Trolox Equivalent Antioxidant Capacity (TEAC) value was (5.824±0.234) mmol/g. The ethyl acetate-extracts, at the concentration of 0.50 mg/mL, had >50% scavenging rate according to the ABTS radical-scavenging assay. The ethyl acetate-extracts also showed outstanding resistant activity against Staphylococcus aureus, the MIC and MBC were 0.313 mg/mL and 0.625 mg/mL, respectively. It was confirmed that the ethyl acetate-extracts from Manilkara zapota leaves showed outstanding antioxidant activity and antibacterial activity, and they could be used in the exploring of natural antioxidants and antibacterial agents.

Publication Date

5-28-2021

First Page

144

Last Page

148

DOI

10.13652/j.issn.1003-5788.2021.05.027

Recommended Citation

Fei-yue, MA; Ming, ZHANG; Ya, LI; Li-qing, DU; and Xi-xiang, SHUAI (2021) "Antioxidant and antibacterial activities of different polar parts from Manilkara zapota leaves," Food and Machinery: Vol. 37: Iss. 5, Article 27.
DOI: 10.13652/j.issn.1003-5788.2021.05.027
Available at: https://www.ifoodmm.cn/journal/vol37/iss5/27

References

[1] 吴涛, 张慧贤, 张方州, 等. 人心果不同溶剂提取物的抗氧化活性研究[J]. 南方农业, 2019, 13（27）: 125-128, 140.
[2] WANG Hong-yu, LIU Ting-ting, SONG Li-xia, et al, Profiles and α-amylase inhibition activity of proanthocyanidins in unripe Manilkara zapota （Chiku） [J]. J Agric Food Chem, 2012, 60（12）: 3 098-3 104.
[3] FAYEK N R, MONEM A R, MOSSA M Y, et al, Chemical and biological study of Manilkara zapota （L.） Van royen leaves （sapotaceae） cultivated in egypt[J]. Pharmacognosy Research, 2012, 4（2）: 85-91．
[4] MA Jun, LUO Xiao-dong, PETR P, et al, Bioactive novel polyphenols from the fruit of Manilkara zapota （Sapodilla） [J]. J Nat Prod, 2003, 66: 983-986.
[5] 冯慧玲. 人心果叶缩合单宁分离纯化及其结构与生物活性的鉴定[D]. 厦门: 厦门大学, 2014.
[6] HERNNDEZ B G I, DZUL R R E, VELZQUEZ M G M, et al. The influence of drying temperatures on the metabolic profiles and antioxidant activity of Manilkara zapota leaves[J]. Metabolites, 2019, 9（10）: 217-225.
[7] SARA T A, ABDULRAHMAN K A, ABDULLAH K A, et al. Protective effect of lyophilized sapodilla （Manilkara zapota） fruit extract against CCl₄-induced liver damage in rats[J]. Saudi Journal of Biological Sciences, 2020, 27（9）: 2 373-2 379.
[8] 张秀梅, 骆党委, 朱祝英, 等. 3种热带果树叶子甲醇粗提物的抗氧化活性比较[J]. 核农学报, 2015, 29（1）: 95-100.
[9] 陈海芳, 付琼, 马飞跃, 等. 热带果树叶子的抗氧化性评价[J]. 中国热带农业, 2016（3）: 45-47.
[10] 马飞跃, 张秀梅, 刘玉革, 等. 人心果叶片提取物抗氧化活性评价[J]. 中国南方果树, 2016, 45（6）: 79-82.
[11] ANWESA C, SANJIB R. In vitro free radical scavenging activities of aerial parts’ aqueous extract and extract fractions of Ampelocissus latifolia （Roxb.） Planch in relation to total phenolics and flavonoid contents[J]. Journal of King Saud University-Science, 2020, 32（1）: 732-739.
[12] 禄璐, 米佳, 罗青, 等. 枸杞总黄酮提取工艺优化及其体外抗氧化活性分析[J]. 食品工业科技, 2019, 40（24）: 165-171.
[13] MA Fei-yue, GU Cheng-bo, LI Chun-ying, et al. Microwave-assisted aqueous two-phase extraction of isoflavonoids from Dalbergia odorifera T. Chen leaves[J]. Separation and Purification Technology, 2013, 115: 136-144.
[14] 侯春莲. 木豆叶中牡荆苷与异牡荆苷提取纯化工艺及其抗菌活性研究[D]. 哈尔滨: 东北林业大学, 2006: 48-50.
[15] 薛雅荣, 薛海波, 郝丽琴, 等. 菠萝皮渣醇提物不同极性部位抗氧化活性研究[J]. 食品研究与开发, 2016, 37（20）: 8-11.
[16] 康兆勇, 李玉霜, 吴过, 等. 厚朴叶不同极性溶剂萃取物总酚含量及体外抗氧化活性研究[J]. 食品研究与开发, 2019, 40（24）: 73-79.
[17] 邱珊莲, 林宝妹, 张少平, 等. 嘉宝果嫩叶提取物不同极性部位抗氧化及抑制α-葡萄糖苷酶活性研究[J]. 热带作物学报, 2019, 40（7）: 1 373-1 378.
[18] 孙霁寒, 黄玲艳, 顾嘉昌, 等. 豆腐柴提取物不同极性部位的总酚、总黄酮含量及体外抗氧化活性[J]. 食品工业科技, 2017, 38（15）: 55-58.
[19] 刘刚, 姜唯唯, 吴京, 等. 芒果叶不同极性部位提取物的抗氧化活性[J]. 食品研究与开发, 2014, 35（11）: 10-14.
[20] 刘永玲, 叶思帆, 杨怡, 等. 野地瓜茎正丁醇萃取物中主要抗氧化活性成分的筛选[J]. 食品与机械, 2020, 36（6）: 176-181.
[21] 吴峰华, 刘相真, 杨虎清, 等. 覆盆子醇提物及其不同极性部位抗氧化活性研究[J]. 中国食品学报, 2012, 12（2）: 24-29.
[22] 黄越, 周春晖, 戴宏杰, 等. 猴头菇醇提物及其不同极性部位的体外抗氧化活性[J]. 食品工业科技, 2017, 38（21）: 16-20.
[23] 尹显楼, 詹济华, 谭洋, 等. 铁苋菜不同极性萃取物的抗氧化及抑菌活性研究[J]. 食品与机械, 2019, 35（6）: 172-176.
[24] ARAJO F M, DANTAS M C, SILVA L S, et al. Antibacterial activity and chemical composition of the essential oil of Croton heliotropiifolius Kunth from Amargosa Bahia, Brazil[J]. Ind Crop Prod, 2017, 105: 203-206.
[25] WEI Zuo-fu, ZHAO Ren-na, DONG Li-jia, et al. Dual-cooled solvent-free microwave extraction of Salvia officinalis L. essential oil and evaluation of its antimicrobial activity[J]. Ind Crop Prod, 2018, 120: 71-76.