Extraction and membrane separation of pigment from seed coat of red adzuki bean

Authors

JIN Li-mei, College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China; Heilongjiang Engineering Research Center for Coarse Cereals Processing and Quality Safety, Daqing, Heilongjiang 163319, China; Agri-Food Processing and Engineering Technology Research Center of Heilongjiang Province, Daqing, Heilongjiang 163319, China
SUI Shi-you, College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China
REN Meng-ya, College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China
BAI Jing, College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China; National Coarse Cereals Engineering Research Center, Daqing, Heilongjiang 163319, China
NIU Guang-cai, College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China; Agri-Food Processing and Engineering Technology Research Center of Heilongjiang Province, Daqing, Heilongjiang 163319, China
LI Zhi-jiang, College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China; Heilongjiang Engineering Research Center for Coarse Cereals Processing and Quality Safety, Daqing, Heilongjiang 163319, China

Abstract

The red pigment from the seed coats of red adzuki beans was extracted by soaking in water and separated by the pressure-driven membrane technology (microfiltration, nanofiltration, etc.), and the separation processes were also optimized. The results showed that the optimal extraction conditions of pigment from seed coats were as follows: the ratio of material to liquid (mass of seed coats: volume of water) at 1∶30 （g/mL）, extraction temperature 90 ℃, extraction time 90 min, and the color value of the extracts reached 7.53. The optimized membrane separation process was the combination of microfiltration (0.22 μm) and nanofiltration membrane (NF245), with the microfiltration pressure at 0.075 MPa the membrane flux speedat 12.47 L/(m²·h), and the pigment transmission rate at 87.16%; the optimal pressure for NF was 0.3 MPa, and the stable flux was 16.06 L/(m²·h). The content of pigment in the NF concentrate increased to 810.19 mg/L and the concentrations of total phenol, total sugar and protein were further reduced. The content of anthocyanin in the purified anthocyanin freeze-dried powder was （145.80±0.17） mg/g. The color value of the purified anthocyanin was 58.08±0.09, which was 2.41 times of that before purification.

Publication Date

5-28-2021

First Page

149

Last Page

155

DOI

10.13652/j.issn.1003-5788.2021.05.028

Recommended Citation

Li-mei, JIN; Shi-you, SUI; Meng-ya, REN; Jing, BAI; Guang-cai, NIU; and Zhi-jiang, LI (2021) "Extraction and membrane separation of pigment from seed coat of red adzuki bean," Food and Machinery: Vol. 37: Iss. 5, Article 28.
DOI: 10.13652/j.issn.1003-5788.2021.05.028
Available at: https://www.ifoodmm.cn/journal/vol37/iss5/28

References

[1] SELIG M J, CELLI G B, TAN C, et al. High pressure processing of beet extract complexed with anionic polysaccharides enhances red color thermal stability at low pH[J]. Food Hydrocolloids, 2018, 80: 292-297.
[2] 段晓嫣, 田艳, 邓放明. 火龙果色素生物活性及其提取纯化研究进展[J]. 食品与机械, 2017, 33（10）: 214-219.
[3] 王心哲, 孟祥敏, 游颖, 等. 微波辅助水提法提取黑米花色苷的工艺研究[J]. 粮食与油脂, 2020, 33（1）: 94-96.
[4] KAWAKAMI W, OSHIMA A, YANASE E. Structural characterization of proanthocyanidins from adzuki seed coat[J]. Food Chemistry, 2018, 239: 1 110-1 116.
[5] AMAROWICZ R, ESTRELLA I, TERESA Hernandez, et al. Antioxidant activity of extract of adzuki bean and its fractions[J]. Journal of Food Lipids, 2008, 15（1）: 119-136.
[6] 金丽梅, 白静, 隋世有, 等. 响应面法优化微波辅助提取红小豆种皮花色苷及其稳定性研究[J]. 食品工业科技, 2021, 42(6): 187-194.
[7] 韩涛, 甘育新, 李丽萍, 等. 红小豆种皮红色素的提取及其理化性质的研究[J]. 中国粮油学报, 1997, 12（6）: 58-62.
[8] 刘雪雁, 马爱红, 杨昌群, 等. 红豆皮提取天然色素的实验室研究[J]. 吉林工学院学报（自然科学版）, 1999（2）: 31-33.
[9] 向明锋, 罗璇, 沈瑞敏, 等. 小曲酒酿造废水中高粱红色素的分离纯化工艺研究[J]. 中国酿造, 2018, 37（4）: 127-131.
[10] 房照龙, 扈本荃. 现代提取技术在天然色素领域的应用[J]. 广州化工, 2018, 46（8）: 21-23.
[11] 施燕. 膜分离技术在天然植物提取中的应用[C]// 第二届全国膜分离技术在食品工业中应用研讨会论文集. 北京: 膜科学与技术, 2006: 32-34.
[12] 马乐, 韩军歧, 张润光, 等. 大孔吸附树脂在植物多酚分离纯化中的应用现状[J]. 食品工业科技, 2015, 36（12）: 364-367, 374.
[13] 尹忠平, 上官新晨, 黎冬明, 等. 花青素类色素纯化技术研究进展[J]. 粮油加工, 2010（7）: 111-115.
[14] 王金秋, 朱倩, 郁蓓蕾, 等. 食品工业中膜分离技术的应用进展[J]. 成都大学学报（自然科学版）, 2017, 36（3）: 252-256.
[15] 李媛媛, 高彦祥. 膜分离技术纯化栀子黄色素的研究[J]. 食品科学, 2006, 27（6）: 113-117.
[16] 胡建农, 郑晓英. 膜技术浸提玫瑰茄红色素工艺的初步研究[J]. 亚热带植物科学, 2004, 33（3）: 19-21.
[17] 韩涛, 甘育新, 李丽萍, 等. 红小豆种皮红色素的提取及其理化性质的研究[J].中国粮油学报，1997, 12（6）: 58-62.
[18] 李洋, 韩小贤, 成军虎, 等. 基于正交试验法优化红小豆色素提取条件研究[J]. 农产品加工学刊, 2011（7）: 23-25.
[19] 徐菀璐, 罗华, 来明月, 等. 超声波辅助提取玫瑰花色素及其稳定性研究[J]. 食品安全质量检测学报, 2018, 9（11）: 2 830-2 835.
[20] 张小曼, 马银海, 李勇, 等. 膜分离技术提取山竺红色素的工艺优化[J]. 食品科学, 2010, 31（10）: 133-136.
[21] 宋德群, 孟宪军, 王晨阳, 等. 蓝莓花色苷的pH示差法测定[J]. 沈阳农业大学学报, 2013, 44（2）: 231-233.
[22] 徐辉艳, 孙晓东, 张佩君, 等. 红枣汁中总酚含量的福林法测定[J]. 食品研究与开发, 2009, 30（3）: 126-128.
[23] 杨勤, 谷文超, 周浓, 等. 苯酚—硫酸法与蒽酮—硫酸法测定地参多糖的比较研究[J]. 食品科技, 2020, 45（1）: 343-345.
[24] 蒋大程, 高珊, 高海伦, 等. 考马斯亮蓝法测定蛋白质含量中的细节问题[J]. 实验科学与技术, 2018, 16（4）: 143-147.
[25] 邸翔, 刘长涛, 高莉, 等. 红果小檗色素的提取及其稳定性研究[J]. 食品与发酵工业, 2010, 36（6）: 184-189.
[26] 龚金华, 张玥, 张智超, 等. 现代分离纯化技术在天然植物色素中的应用[J]. 中国食物与营养, 2014, 20（12）: 54-57.
[27] 余以刚, 梁泽明, 万志超, 等. 玫瑰茄花色苷的纯化及其热降解稳定性[J]. 现代食品科技, 2018, 34（12）: 58-66.
[28] 徐忠, 张丽亚. 大孔树脂对红豆皮色素的吸附性能研究[J]. 食品工业科技, 2002, 23（7）: 35-36.
[29] BELLONA C, DREWES J E, XU Pei, et al. Factors affecting the rejection of organic solutes during NF/RO treatment: A literature review[J]. Water Research, 2004, 38: 2 795-2 809.
[30] 刘志强, 张初署, 孙杰, 等. 膜分离技术纯化花生衣中的原花色素[J]. 食品科学, 2010, 31（20）: 183-187.