Determination of 19 antidepressants in health food by high-performance liquid chromatography-Time-of-flight mass spectrometry

Authors

LIU Jie, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
ZHU Xiao-ling, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
PENG Qing-zhi, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
ZHOU Tao-hong, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
GONG Lei, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China
WU Wan-qing, Hubei Provincial Institute for Food Supervision and Test, Wuhan, Hubei 430070, China; Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan, Hubei 430070, China

Abstract

Objective: To establish a rapid qualitative and quantitative analysis method, HPLC-quadrupole-time-of-flight mass spectrometry was used to determine the illegal addition of 19 antidepressants in healthy food. Methods: The samples were extracted by methanol with ultrasonic extraction procedure and then separated on a reversed-phase C₁₈ column, with acetonitrile and 0.1% formic acid aqueous as the mobile phases in gradient elution mode. The target compounds were detected in electrospray positive ion, and the data were matched with the established database of precursor ion and secondary fragment accurate mass. Results: The linear range of the 19 compounds was 5~100 μg/L, and the correlation coefficient was 0.988 7~0.999 9, with the detection limit of 0.2~2.0 μg/kg. The average recoveries of the 19 antidepressants ranged from 81.2% to 98.6%, and the relative standard deviation was less than 6.7%. Conclusion: The established method is suitable for rapid screening qualitative and quantitative analysis of illegally added antidepressant drugs in healthy food.

Publication Date

7-28-2021

First Page

63

Last Page

68

DOI

10.13652/j.issn.1003-5788.2021.07.010

Recommended Citation

Jie, LIU; Xiao-ling, ZHU; Qing-zhi, PENG; Tao-hong, ZHOU; Lei, GONG; and Wan-qing, WU (2021) "Determination of 19 antidepressants in health food by high-performance liquid chromatography-Time-of-flight mass spectrometry," Food and Machinery: Vol. 37: Iss. 7, Article 10.
DOI: 10.13652/j.issn.1003-5788.2021.07.010
Available at: https://www.ifoodmm.cn/journal/vol37/iss7/10

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