Simultaneous rapid determination of 18 quinolones residues in raw milk by liquid-liquid extraction and ultra performance liquid chromatography tandem mass spectrometry

Authors

ZHOU Yan-hua, Changsha Environmental Protection College， Changsha， Hunan 410111， China
LI Tao, Hunan Provincial Food Quality Supervision and Inspection Institute, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning， Changsha, Hunan 410111, China
PAN Xiao-hong, Hunan Institute for Pharmaceutical Inspection, Changsha, Hunan 410001, China
XIANG Jun, Hunan Provincial Food Quality Supervision and Inspection Institute, Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning， Changsha, Hunan 410111, China

Abstract

Objective: A rapid method for simultaneous determination of 18 quinolones residues in raw milk was established. Methods: The raw milk was pretreated by liquid-liquid extraction, and the residues of 18 quinolones in raw milk were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry. Results: The linearity of all the 18 quinolones from 2.0 to 100.0 ng/mL had correlation coefficients greater than 0.992. The limits of detection (LOD) of this method ranged from 0.02 to 0.50 μg/kg, and the limits of quantification (LOQ) were 0.06 to 1.50 μg/kg. The average recoveries of the 18 quinolones were 74.0% to 106.5%, with the relative standard deviation （n=6） of 1.1%~13.0%. Conclusion: The method is simple, efficient and accurate, and can be used for the quantitative confirmation of quinolones residues in raw milk.

Publication Date

8-28-2021

First Page

63

Last Page

69,76

DOI

10.13652/j.issn.1003-5788.2021.08.010

Recommended Citation

Yan-hua, ZHOU; Tao, LI; Xiao-hong, PAN; and Jun, XIANG (2021) "Simultaneous rapid determination of 18 quinolones residues in raw milk by liquid-liquid extraction and ultra performance liquid chromatography tandem mass spectrometry," Food and Machinery: Vol. 37: Iss. 8, Article 10.
DOI: 10.13652/j.issn.1003-5788.2021.08.010
Available at: https://www.ifoodmm.cn/journal/vol37/iss8/10

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